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Objective: To investigate the expression of long-chain non-coding RNA LINC00472 in renal fibrotic cell models and its functional role in renal tubular epithelial cell fibrosis and epithelial-mesenchymal transition (EMT) induced by TGF-β1. Methods: Recombinant human TGF-β1 was used to induce fibrosis and EMT in human renal tubular epithelial cells (HK-2). Real-time fluorescent quantitative PCR was used to detect the expression levels of fibrosis-related genes and LINC00472 mRNA in the cells, and Western blot was used to Western blot. Method was used to detect the protein expression level of fibrosis-related genes in cells, and the effect of LINC00472 expression on the migration ability of HK-2 cells was evaluated by scratch experiment. Results: TGF-β1 can successfully induce fibrosis and EMT in HK-2 cells, and inhibit the expression of LINC00472 in a dose- and time-dependent manner (P<0.05). Inhibition of LINC00472 further promoted the up-regulation of Fibronectin and Vimentin induced by TGF-β1, and the down-regulation of E-cadherin (P<0.05); while overexpression of LINC00472 could reverse the inducing effect of TGF-β1 on fibrosis-related genes (P<0.05). In addition, inhibiting LINC00472 can further enhance the HK-2 cell migration induced by TGF-β1 (P<0.05), while upregulating LINC00472 can inhibit cell migration (P<0.05). Conclusion: LINC00472 has a low expression in TGF-β1 induced renal fibrosis cell model, and the decrease of its expression level can promote cell fibrosis and EMT process. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the clinical effect of tissue expander assisted neuroendoscopy in the treatment of hypertensive intracerebral hemorrhage, and to provide evidence for the treatment of hypertensive intracerebral hemorrhage. Methods: 108 patients with hypertensive intracerebral hemorrhage who were treated in our hospital fromFebruary 2017 to January 2018 were selected. Among them, 52 patients with hypertensive intracerebral hemorrhage treated with tissue expander assisted neuroendoscopy were selected as the study group. 56 patients with hypertensive intracerebral hemorrhage treated by traditional craniotomy and evacuation of hematoma were used as the control group. The bleeding volume, hematoma clearance rate, operation time, postoperative rebleeding rate, postoperative complications and Glasgow outcome score (GOS) at 3 months after operation and 6 months after operation were compared between the two groups. Results: The operation time of the study group was shorter than that of the control group. The bleeding volume and rebleeding rate of the study group were lower than those of the control group, and the hematoma clearance rate was higher than that of the control group, the difference was statistically significant (P<0.05). The incidence of pulmonary infection and urinary tract infection in the study group were significantly lower than those in the control group (P<0.05). The incidence of gastrointestinal bleeding and deep venous thrombosis in the two groups were not statistically significant (P>0.05). The distribution of GOS score in the two groups at 6 months after operation was better than that at 3 months after operation. The distribution of GOS score at 3 months after operation and 6 months after operation in the study group were better than that of the control group (P<0.05). Conclusions: Tissue dilator assisted neuroendoscopy in the treatment of hypertensive cerebral hemorrhage can effectively shorten the operation time and reduce the amount of bleeding volume. Its hematoma clearance rate is also higher. The incidence of postoperative complications is low and the prognosis is good. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression levels and clinical significance of von Willebrand factor(v WF), granule membrane protein-140(GMP-140), von Willebrand factor cleaving protease(ADAMTS13) in patients with aneurysmal subarachnoid hemorrhage(aSAH). Methods: 83 patients with aSAH treated in neurosurgery of our hospital from January 2014 to December 2016 were selected.They were divided into 37 cases of cerebral vasospasm(CVS) group and 46 cases of non CVS group, and 31 cases of delayed cerebral ischemia(DCI) group and 52 cases of non DCI group. They were divided into 43 cases of <5 mm group, 29 cases of 5-10 mm group, 11 cases of >10 mm group according to the diameter of different aneurysms, and divided into 49 cases of good prognosis group and 34 cases of poor prognosis group. The levels of plasma v WF, GMP-140 and ADAMTS13 in patients with aSAH were detected,and the correlation between the indexes were analyzed. Results: The plasma levels of v WF in CVS group were higher than that in non CVS group on the fourth day, tenth day, the plasma levels of GMP-140 in CVS group were higher than that in non CVS group on the first day, fourth day,tenth day, the plasma levels of ADAMTS13 in CVS group were lower than that in non CVS group on the first day, tenth day, the differences were statistically significant(P<0.05). The plasma levels of v WF in DCI group were higher than that in non DCI group on the first day, the levels of ADAMTS13 were lower than that in non DCI group, the plasma levels of v WF and GMP-140 were higher than that in non DCI group on the fourth day, the differences were statistically significant(P<0.05). The plasma levels of v WF and GMP-140 in >10 mm group were higher than that in <5 mm group and 5-10 mm group on the first day, fourth day, the plasma levels of v WF on the fourth day and GMP-140 levels on the first day were higher than that in <5 mm group, the differences were statistically significant(P<0.05). The plasma levels of ADAMTS13 in >10 mm group were lower than that in <5 mm group and 5-10 mm group on the first day, <5 mm group were lower than 5-10 mm group, the differences were statistically significant(P<0.05). The plasma levels of v WF in good prognosis group were lower than that in poor prognosis group on the fourth day, tenth day, the plasma levels of GMP-140 in good prognosis group were lower than that in poor prognosis group on the first day, fourth day, tenth day, the plasma levels of ADAMTS13 in good prognosis group were higher than that in poor prognosis group on the first day, fourth day, the differences were statistically significant(P<0.05).Pearson correlation analysis showed that plasma v WF was positively correlated with GMP-140 and negatively correlated with ADAMTS13, GMP-140 was negatively correlated with ADAMTS13 on the first day, fourth day(r=0.334,-0.426,-0.398, 0.278,-0.311,-0.235, P<0.05). There was no correlation between the plasma levels of v WF, GMP-140, ADAMTS13 on the tenth day(P>0.05). Conclusion: The plasma levels of VWF, GMP-140, ADAMTS13 and CVS, DCI, aneurysm diameter and prognosis are closely related, joint detection helps to comprehensively assess the condition of patients with aSAH, improve prognosis, it is worthy of clinical promotion. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective: To explore the influence of sufentanil patient-controlled intravenous analgesia on postoperative hemodynamics and immune function in patients with lung cancer resection. Methods: A total of 110 patients with non-small cell lung cancer(NSCLC), who underwent lobectomia pulmonalis in Seventh Affiliated Hospital of Zunyi Medical College from February 2009 to February 2014, were selected and randomly divided into observation group(n=55) and control group(n=55). The observation group was treated with sufentanil patient-controlled intravenous analgesia after surgery, while the control group, fentanyl patient-controlled intravenous analgesia after surgery.Then observed the difference of hemodynamics indexes and immune function indexes between the two groups. Results: The whole blood viscosity, whole blood reduction viscosity, red blood cell aggregation index(RAI) and capillary plasma viscosity in the observation group 1 d after analgesia were significantly lower than those in the control group(P<0.05).Contents of IL-2 and CD4+T cell in the observation group were significantly higher than those in the control group, and the contents of IL-6, IL-10 and CD8+T cells were significantly lower than those in the control group(P<0.05). Conclusion: Sufentanil patient-controlled intravenous analgesia can significantly improve the hemodynamics indexes and enhance immune function, which is worthy of clinical application. [ABSTRACT FROM AUTHOR]

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Progress in Modern Biomedicine is the property of Publishing House of Progress in Modern Biomedicine and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)