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To explore the expression of inflammatory factors and TSP-1 in gingival crevicular fluid in patients undergoing orthodontic extraction, and the influencing factors of periodontal disease. Eighty patients admitted to our hospital from August 2020 to August 2023 for orthodontic treatment of extracted teeth were retrospectively analyzed, and gingival sulcus fluid samples were taken from all the patients before orthodontic treatment, 1 month after orthodontic treatment, 3 months after orthodontic treatment, and at the end of orthodontic treatment to detect the tumor necrosis factor-alpha (TNF-alpha), interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and platelet reactive protein- 1 (TSP-1) expression levels. Subsequently, the patients were divided into periodontal disease group (n=36) and non-periodontal disease group (n=44) according to whether they suffered from periodontal disease during orthodontic treatment, compared the expression levels of TNF-琢, IL-1茁, IL-6, and TSP-1 of the patients in the two groups before orthodontic treatment and at the end of orthodontic treatment, and compared the general conditions of the patients in the two groups, and then analyzed the influence factors of periodontal diseases in patients with extracted teeth in orthodontics with the use of logistic regression model. Disease Influencing Factors. TNF-琢, IL-1茁, IL-6, and TSP-1 levels were elevated at 1 month and 3 months after orthodontic treatment in 80 patients with extracted teeth, and TNF-琢, IL-1茁, IL-6, and TSP-1 levels leveled off from 3 months to the end of orthodontic treatment, but were significantly higher than those before orthodontic treatment at 1 month, 3 months, and at the end of orthodontic treatment (P<0.05); there were significant differences in the expression levels of TNF-琢, IL-1茁, IL-6, and TSP-1 in patients in the periodontal diseases group compared to those in the non periodontal diseases group, and the expression levels in the periodontal diseases group were significantly higher than those in the non periodontal diseases group (P<0.05). There were significant differences in the expression levels of TNF-琢, IL-1茁, IL-6, and TSP-1 between patients in the pre-orthodontic and post-orthodontic groups, and the periodontal disease group was significantly higher than the non-periodontal disease group (P<0.05); there were no significant differences in the comparison of gender, age, and BMI between patients in the periodontal disease group and the non-periodontal disease group (P>0.05), and there were no significant differences in the duration of orthodontic treatment, number of extractions, oral cavity, oral cavity, and oral health of patients in the periodontal disease group and the non-periodontal disease group (P<0.05). number, orthodontic method, and oral cleanliness were compared significantly (P<0.05); logistic regression analysis showed that TNF-琢, IL-1茁, IL-6, TSP-1, orthodontic method, and oral cleanliness were independent risk factors for periodontal disease in patients with extracted teeth and orthodontic treatment (P<0.05). Inflammatory factors of gingival crevicular fluid and TSP-1 levels increased significantly in orthodontic patients with prolonged orthodontic time, and TNF-琢, IL-1 茁, IL-6, TSP-1, orthodontic mode and oral cleanliness were independent risk factors for periodontal disease in orthodontic patients. [ABSTRACT FROM AUTHOR]

Objective: To observe the effect of low dose doxycycline combined with intense pulsed light on skin lesion symptoms, inflammatory reaction and acne specific quality of life (Acne-QOL) score in patients with moderate to severe acne. Methods: According to the method of random number table, 97 patients with moderate to severe acne who were received inYixing Hospital Affiliated to Jiangsu University from March 2020 to May 2021 were divided into the control group (low dose doxycycline treatment, n=48) and the study group (n=49, low dose doxycycline combined with intense pulsed light treatment). The curative effect, skin lesion symptoms, inflammatory reaction and Acne-QOL score of the two groups were compared. Results: After treatment, the total clinical effective rate of the study group (93.88%) was higher than that of the control group (77.08%) (P<0.05). The number of comedones, inflammatory mounds, pustules, cysts and nodules decreased, and the study group was lower than the control group at the same period (P<0.05). Tumor necrosis factor after treatment-α (TNF-α), high sensitivity C-reactive protein (hs-CRP), interleukin-8 (IL-8) and procalcitonin (PCT) decreased, and the study group was lower than the control group at the same period (P<0.05). The scores of emotional function, self perception, social function and acne symptoms of the two groups after treatment increased, and the study group was higher than the control group at the same period (P<0.05). There was no difference in the incidence of adverse reactions in the two groups (P>0.05). Conclusion: Low dose doxycycline combined with intense pulsed light can effectively improve the skin lesion symptoms in patients with moderate to severe acne, reduce the inflammatory reaction of the body, which is conducive to improving the quality of life of patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate the therapeutic effect of human Amniotic Epithelial cells(hAECs) combined with platelet rich Plasma(PRP) on intrauterine adhesion in rats, so as to provide a theoretical basis for optimizing the treatment of intrauterine adhesion in clinical treatment. Methods: 40 SPF female SD rats were randomly divided into blank group(group A), sham operation group(group B), hAECs group(group C), PRP group(group D) and hAECs+PRP group(group E) according to the random number table method, with 8 rats in each group. Group A did not do any operation, group B did anesthesia and open surgery, groups C, D, E used scratching method to make intrauterine adhesion model. Subsequently, groups C was treated with hAECs, and group D was injected with the same volume of normal saline. Groups D was treated with PRP, and group C was injected with the same volume of normal saline.Group E was treated with hAECs combined with PRP. The protein expression levels of MMP-9 and DDR2, related m RNA expression levels of PI3K/Akt/mTOR pathway, serum levels of IL-6 and IL-8, and the number of gestational sacs in each group were observed and compared between the two groups. Results: Compared with group A, there were no differences in the protein expression levels of MMP-9and DDR2, p-MTOR m RNA and p-Akt m RNA levels, and serum IL-6 and IL-8 levels in group B(P＞0.05). The protein expression levels of MMP-9 and DDR2 in endometrial tissues of rats in groups C, D and E were decreased, while the levels of IL-6 and IL-8, p-mTOR m RNA and p-Akt m RNA were increased(P＜0.05). The protein expression levels of MMP-9 and DDR2 in group E were significantly higher than those in groups C and D, and the levels of IL-6 and IL-8, p-mTOR m RNA and p-Akt m RNA were lower than those in groups C and D(P＜0.05). There were no differences in the protein expression levels of MMP-9 and DDR2, serum IL-6 and IL-8 levels, and the m RNA expression of p-mTOR and p-Akt between groups C and D(P＞0.05). In terms of the number of gestational sacs, compared with group A,the number of gestational sac of rats in group B. Conclusion: hAECs combined with PRP may plays a therapeutic role by upregulation of MMP-9 and DDR2 protein expression levels and down-regulation of P-m TOR m RNA and p-Akt m RNA expression in endometrial tissue of rats with intrauterine adhesion, and decreasing the levels of inflammatory factors IL-6 and IL-8, thereby increasing the number of gestational sacs in pregnancy. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of nasal high flow oxygen therapy on blood gas index, diaphragm function and inflammatory reaction in patients with acute exacerbation of chronic obstructive pulmonary disease (AECOPD). Methods: The clinical data of 119 patients with AECOPD in our hospital from February 2018 to August 2020 were retrospectively analyzed. According to different oxygen therapy methods, they were divided into traditional group with 59 cases and study group with 60 cases. The traditional group was treated with traditional oxygen therapy, and the study group was treated with nasal high flow oxygen therapy. The changes of blood gas index, diaphragm function and inflammatory reaction were compared between the two groups before and after treatment. The incidence of adverse reactions, reintubation rate after treatment, disappearance rate of dyspnea and chest tightness, expectoration difficulty were observed. Results: Arterial blood carbon dioxide partial pressure (PaCO2) in the study group after treatment was lower than that in the traditional group, blood oxygen saturation (SaO2), arterial partial pressure of oxygen (PaO2) were higher than those in the traditional group (all P<0.05). After treatment, diaphragm shallow fast respiration index (D-RSBI), diaphragm movement of calm breathing (DEq) in the study group were lower than those in the traditional group (all P<0.05). After treatment, the levels of serum interleukin (IL)-6, tumor necrosis factor (TNF)-α in the study group were lower than those in the traditional group (all P<0.05). The reintubation rate in the study group after treatment was lower than that in the traditional group, and the disappearance rate of dyspnea, chest tightness and the disappearance rate of expectoration difficulty in the study group were higher than those in the traditional group (all P<0.05). There was no significant difference in the total incidence of adverse reactions between the two groups (P>0.05). Conclusions: Compared with traditional oxygen therapy, nasal high flow oxygen therapy in patients with AECOPD can effectively improve blood gas indexes, diaphragm function, reduce inflammatory response, and improve the clinical symptoms of patients. [ABSTRACT FROM AUTHOR]

Objective: To study the clinical effect of acupuncture, massage and external application of traditional Chinese medicine on lumbar disc herniation and comparison of serum TXB2, IL-1 β and IL-10 levels. Methods: 80 lumbar disc protrusion who received therapy from January 2019 to December 2020 in our hospital were selected as research objects. According to random number table, those patients were divided into the observation group (n=41) and the control group (n=39). The control group was treated with external application of traditional Chinese medicine. The observation group was treated with acupuncture, massage and external application of traditional Chinese medicine. The therapeutic effects, JOA and VAS scores, serum TXB2, IL-1 β and IL-10 levels, lumbar function and lumbar joint range of motion, and the incidence of adverse reactions were compared between the two groups. Results: After treatment, The total effective rate of observation group was significantly higher than that of control group[95.12%(39/41) vs 71.79％(28/39)](P＜0.05). JOA was significantly higher than that of the control group[(23.19±3.21)scores vs (17.62±2.65)scores](P＜0.05). VAS score was significantly lower than that of the control group[(2.07±0.38)scores vs（3.58±0.61）scores] (P＜0.05). The levels of TXB2 and IL-1 β in serum were significantly lower than those in control group [(24.37±3.26) μg/L vs (34.08±4.72) μg/L, (0.12±0.03) ng/L vs (0.27±0.05) ng/L] (P＜0.05). Serum IL-10 level was significantly lower than that of the control group[(85.82±7.03) pg/mL vs (57.28±6.31) pg/mL] (P<0.05). Oswestry score was significantly lower than that of the control group[(37.81±6.23)％ vs(68.02±8.91)％] (P<0.05). The range of motion of the lumbar joint was significantly higher than that of the control group[(80.36±0.82)° vs(71.27±0.6) °] (P<0.05). There was no significant difference in adverse reactions between the two groups (P>0.05). Conclusion: Acupuncture, massage and external application of traditional Chinese medicine have significant clinical effect on lumbar disc herniation, which can effectively improve the clinical symptoms of patients, relieve pain, inhibit the expression of inflammatory factors TXB2 and IL-1 β, and promote the level of anti-inflammatory factor IL-10, which is safe and effective. [ABSTRACT FROM AUTHOR]

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From the perspective of depression-like behavior and muscle inflammatory response, the curative effect of curcumin in two excipient carriers of cocoa butter and peanut oil on chronic stress rats was studied. Curcumin preparations were prepared with cocoa butter and peanut oil as excipients respectively, and 50 male SD rats were randomly divided into blank control group, model control group, curcumin cocoa butter group, curcumin peanut oil group, and positive control group. Except for the blank control group, each group used the methods of restraint, noise and sound stimulation, ice water bath stimulation, tail pain stimulation, etc. for 6 weeks to establish a chronic stress rat model. Each was given various design drugs based on maintaining the model stimulation conditions. After 7 days of continuous medication, the behavioral changes of the tail suspension struggling time, mechanical pain threshold, and sugar water preference of model animals in each group were investigated, and then use the Elisa kit method to detect the content of 5-HT and DA in the brain of each group of rats. Make HE-stained rat masseter tissue sections, observe the pathological changes of muscles, and detecte the levels of HMGB-1, IL-1茁, IL-6, and TNF-琢in masseter muscle. Compared with the model group, both curcumin preparations can significantly reduce the depression-like behavior of stress rats and improve the content of 5-HT and DA in the brain. Compared with the positive control group, the two curcumin in the masseter muscle pathology section group can significantly reduce the inflammatory response around the masseter muscle vessels in stress rats. The two curcumin can significantly reduce the levels of IL-1茁, IL-6 and TNF-琢in masseter muscle tissue (P<0.05), and the curcumin cocoa butter group had the most obvious effect.Both curcumin preparations have a therapeutic effect against depression-like symptoms in chronic stress rats, and can reduce the levels of inflammatory factors HMGB-1, IL-1茁, IL-6, and TNF-琢in masseter muscle. Cocoa butter has a significant effect when used as an excipient, and it has good development potential in the research of curcumin's antidepressant preparations. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of acute normovolemic hemodilution (ANH) autotransfusion and allogeneic transfusion on T cell subsets, liver and kidney function and inflammatory factors levels in patients undergoing cesarean section. Methods: The clinical data of 128 cases of cesarean section in our hospital from July 2016 to August 2019 were analyzed retrospectively. According to the different ways of blood transfusion, the patients were divided into group A (n=64, allogeneic blood transfusion) and group B (n=64, Anh autotransfusion). The blood routine indexes [hemoglobin (Hb), hematocrit (HCT), platelet (PLT), red blood cell (RBC], T cell subsets, inflammatory factors [interleukin-6 (IL-6), C-reactive protein (CRP) and tumor necrosis factor-α(TNF-α)] and indexes of liver and kidney function [aspartate transaminase (AST), Alanine aminotransferase (ALT), Creatinine (CR), Urea nitrogen （BUN）] were compared between the two groups. Results: Hb, HCT, PLT and RBC in group A decreased 5 days after operation (P<0.05), the Hb, HCT, PLT and RBC in group B were higher than those in group A(P<0.05). There was no significant difference in AST, ALT, Cr and BUN between the two groups (P>0.05). CD4+/CD8+, CD4+ decreased and CD8+ increased in group A (P<0.05); CD4+/CD8+ and CD4+ in group B were higher than those in group A, but CD8+ was lower than that in group A (P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0.05). The levels of CRP, IL-6 and TNF-αin group B were lower than those in group A (P<0.05). Conclusion: ANH autotransfusion has no significant effect on T cell subsets, inflammatory reaction, liver and kidney function and blood routine in patients undergoing cesarean section, and it is safe. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of ulinastatin combined with continuous renal replacement therapy (CRRT) on inflammatory reaction, hemodynamic in patients with severe sepsis. Methods: 119 patients with severe sepsis who were admitted to our hospital from May 2015 to April 2019 were selected. All patients were divided into control group (n=59) and study group (n=60) according to random number table. The control group was given CRRT treatment, and the study group was treated with ulinastatin on the basis of the control group. The clinical efficacy, inflammatory reaction indicators and hemodynamic parameters of the two groups were compared. The hospitalization time and fatality rate within 28 days were recorded. Results: The total effective rate of the study group at 7 days after treatment was higher than that of the control group (P<0.05). Serum levels of interleukin-6 (IL-6), procalcitonin (PCT) and tumor necrosis factor-α (TNF-α) decreased at 7 days after treatment of both groups, and those of the study group were lower than those of the control group (P<0.05). Heart rate, mean arterial pressure (MAP), blood lactate decreased and oxygenation index increased at 7 days after treatment of the two groups (P<0.05). Oxygenation index of the study group was higher than that of the control group, while blood lactate was lower than that of the control group (P<0.05). The hospitalization time of the study group was lower than that of the control group (P<0.05), and there was no difference in the fatality rate within 28 days between the two groups (P>0.05). Conclusions: Ulinastatin combined with CRRT is effective in treating the patients with severe sepsis. It can effectively inhibit inflammation, improve hemodynamics and reduce hospitalization time. It has certain clinical application value. [ABSTRACT FROM AUTHOR]

To explore the effect of different doses of ulinastatin combined with atomolan on serum oxidative stress, inflammatory reaction and prognosis in patients with septic shock. 100 patients with septic shock who were treated in our hospital from May 2014 to June 2018 were selected. According to the random number table method, the patients were divided into the ulinastatin group (ulinastatin 200,000 U/d), the atomolam group (atomolam 1.8 g/d), the low-dose combination group (ulinastatin 200,000 U/d and atomolam 1.8 g/d), the high-dose combination group (ulinastatin 400,000 U/d and atomolam 1.8 g/d), with 25 cases in each group. The changes of oxidative stress indexes [malondialdehyde (MDA), advanced oxidation protein products (AOPP), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC), glutathione peroxidase (GSH-Px)] and inflammation indexes[interleukin-6(IL-6), tumor necrosis factor-琢(TNF-琢), procalcitonin (PCT) and c-reactive protein (CRP)] of patients between the three groups before and after treatment were compared. Meanwhile, the differences in prognosis between the three groups were compared. The ICU hospitalization time, mechanical ventilation time, acute physiological and chronic health (APACHE II) scores and sequential organ failure (SOFA) scores, and the level of serum MDA, AOPP, IL-6, TNF-琢, PCT and CRP after treatment of patients in the high-dose combined group were all lower than those in the ulinastatin group, the atomolam group, and the low-dose combined group, while the low-dose combined group were lower than those in the ulinastatin group and the atomolam group (P＜ 0.05). The level of serum SOD, T-AOC, GSH-Px after treatment of patients in the high-dose combined group were all higher than those in the ulinastatin group, the atomolam group, and the low-dose combined group, while the low-dose combined group were higher than those in the ulinastatin group and the atomolam group (P＜ 0.05). The incidence of multiple organ dysfunction (MODS) and mortality in patients of the high-dose combined group and the low-dose combined group were lower than those in the ulinastatin group and the atomolam group (P＜ 0.05). Ulinastatin combined with atomolan is effective in the treatment of dyeability shock. It can effectively inhibit systemic oxidative stress and inflammatory reaction and improve the prognosis of patients, high dose ulinastatin combined with the treatment of septic shock is the best. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of different doses of atorvastatin on cardiac function, blood lipids and serum tumor necrosis factor-α(TNF-α) and atrial natriuretic factor(ANF) levels in patients with coronary heart disease. Methods: 86 cases of coronary heart disease treated in our hospital from January 2016 to January 2017 were selected. The patients were divided into observation group and control group according to the random number table method, 43 cases in each group. The control group was treated with 10 mg dosage of atorvastatin, while the observation group was treated with 20 mg dosage of atorvastatin. The two groups were treated for 6 months. The levels of left ventricular end diastolic diameter(LVEDD), left ventricular ejection fraction(LVEF), left atrium anteroposterior diameter(LAD), total cholesterol(TC), triacylglycerol(TG), high density lipoprotein cholesterol(HDL-C), low density lipoprotein cholesterol(LDL-C), TNF-α and ANF were compared between the two groups before and after treatment. All patients were followed up for one year to compare the occurrence of cardiovascular events such as myocardial infarction, arrhythmia and cardiac death. Results: After treatment, the levels of LVEDD, LAD, TC, TG, LDL-C, TNF-α and ANF in both groups were significantly decreased, while the levels of LVEF and HDL-C were significantly increased(P<0.05). After treatment, the levels of LVEDD, LAD, TC,TG, LDL-C, TNF-α and ANF in the observation group were lower than those in the control group, and the level of LVEF in the observation group was higher than that in the control group(P <0.05). There was no significant difference in the incidence of cardiovascular events between the two groups(P>0.05). Conclusion: Both doses of atorvastatin can effectively improve heart function and blood lipids in patients with coronary heart disease, reduce the inflammatory reaction in patients, but the effect of 20 mg of atorvastatin is better. [ABSTRACT FROM AUTHOR]

Objective: To study the expression level of liver function in patients of cardiac surgery with cardiopulmonary bypass and the intervention mechanism of ademetionine. Methods: A total of 184 patients underwent cardiopulmonary bypass surgery in the Second Affiliated Hospital of Hainan Medical University from May 2017 to June 2018 were selected, and were divided into observation group and the control group according to random number table method, with 92 cases in each group. The liver function indexes levels of all the patients before and after operation were compared respectively. After surgery, the patients in the control group were given saline intervention for 7d, and the patients in the observation group were given ademetionine intervention for 7 d. The liver function indexes,inflammatory response indexes and myocardial injury indexes between the two groups were compared after seven days of intervention.Results: The total bilirubin(TBI), direct bilirubin(DBI), alanine aminotransferase(ALT), aspartate aminotransferase(AST) levels before surgery were significantly lower than those after surgery, the differences were statistically significant(P <0.05). After seven days of intervention, the TBI, DBI, ALT, AST, serum tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8), creatine kinase isoenzyme(CK-MB), cardiac troponin T(cTnT) levels of observation group were significantly lower than those of the control group,and the interleukin-10(IL-10) level of observation group was significantly higher than that of the control group, the differences were statistically significant(P <0.05). The incidence of adverse reactions in the observation group was 7.61%(7/92), and there was no significant difference compared with 2.17%(2/92) in the control group(P>0.05). Conclusion: Cardiopulmonary bypass surgery can cause some damage to liver function, but ademetionine has certain protective effect in the patients’ liver function and myocardial tissue and can reduce the degree of inflammatory reaction at the same time, without serious adverse drug reaction. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Gukang capsule combined with Sodium Hyaluronate Injection treatment of osteoarthritis, and its influence on inflammatory reaction stress. Methods: A total of 110 cases of osteoarthritis patients were selected as the research object, based on the random data table they were divided into control group and observation group, 55 cases in each group, patients in the control group were treated with Sodium Hyaluronate Injection treatment, observation group of patients were treated with Gukang capsule combined with Sodium Hyaluronate Injection treatment, the total effective rate, the clinical symptom score, the inflammatory factor and the incidence of adverse reaction after treatment were compared. Results: The total effective rate of treatment in the observation group was 90.91%, which was significantly higher than that of the control group(70.91%), and the difference was statistically significant(x2=7.308, P<0.05). There was no significant difference in Lysholm score, VAS score, TNF-α and CRP level between the two groups before treatment(P>0.05). The Lysholm score of the observation group was significantly higher than that of the control group.The VAS score, TNF-α and CRP were significantly lower than those in the control group, and the difference was statistically significant(P<0.05). There was no significant difference in the incidence of adverse drug reactions between the two groups(x2=0.121, P=0.728). Conclusion: Gukang capsule combined with Sodium Hyaluronate Injection, can effectively improve the osteoarthritis of clinical therapeutic effect, improve the patients clinical symptoms, reducing the inflammatory response to stress, high safety, which has a certain clinical value. [ABSTRACT FROM AUTHOR]

Objective: To study the expression of plasma soluble endothelial protein C receptor(s EPCR) and nitric oxide(NO) in children with Kawasaki disease and its relationship with inflammatory reaction and coronary artery disease(CAL). Methods: 104 children with Kawasaki disease who were treated in Central Hospital of Panjin from December 2016 to February 2018 were selected as Kawasaki disease group, according to the results of color echocardiography, the children of Kawasaki disease group were divided into CAL group with 30 cases and non CAL group(NCAL group) with 74 cases. In addition, 56 healthy children who were received healthy physical examination in our hospital at the same time were selected as the control group. The levels of s EPCR, NO, C-reactive protein(CRP), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) were compared between the Kawasaki disease group and the control group, the acute stage and remission stage of children in the Kawasaki disease group, the CAL group and the NCAL group. The correlation of s EPCR, NO and inflammatory factors, CAL of Kawasaki disease were analyzed. Results: The levels of s EPCR, NO, CRP, IL-6, TNF-α of Kawasaki disease group were higher than the control group,the difference was statistically significant(P<0.05).The levels of s EPCR, NO, CRP,TNF-α, IL-6 in acute stage of Kawasaki disease group were higher than those in remission stage, the difference was statistically significant(P<0.05). The levels of s EPCR, NO, CRP, TNF-α and IL-6 in CAL group were higher than those in NCAL group, and the difference was statistically significant(P<0.05). The results of Pearson correlation analysis showed that s EPCR and NO were positively correlated with CRP, IL-6, TNF-α and CAL in children with Kawasaki disease(P<0.05). Conclusion: The levels of s EPCR and NO in children with Kawasaki disease are related to inflammatory factors and CAL, s EPCR and NO may play a certain role in its inflammatory response and CAL progression. [ABSTRACT FROM AUTHOR]

Objective: To investigate the curative effect of plasma radiofrequency catheter ablation on adenoid hypertrophy in children and its effect on ventilatory function and inflammatory response. Methods: 100 cases of adenoid hypertrophy in our hospital from December 2016 to August 2017 were selected, 50 cases patienta treated with surgical treatment was in plasma group, and 50 patients treated with conservative treatment were in control group in the same period. The control group was given montelukast and Mumetasone furfurfurate nasal spray, and the plasma group was given low temperature plasma radiofrequency ablation. The symptom score, ventilation function and inflammatory cytokines levels in two groups before and after treatment were compared. Results: Before treatment, symptom score, apnea hypopnea index (АШ), longest apnea time (LAT), oxygen reduction index (ODI), minimum nocturnal oximetry Lowest nocturnal oxygen saturation (LSa02), percentage of time when oxygen saturation less than 90% (SLT 90%), and serum inflammatory mediators (TNF-a), interleukin-4 (IL-4) and eosinophil cationic protein (ECP) levels were not significantly different between the two groups (P>0.05). After treatment, the scores of snoring, nasal obstruction and mouth breathing were significantly decreased in both groups (P<0.05), and the scores of snoring, nasal obstruction and mouth breathing in plasma group were significantly lower than those in control group (P<0.05). After treatment, the levels of АШ, LAT, ODI and SLT90 % in both groups were significantly decreased (P<0.05), and the LSa02 level was significantly increased (P<0.05). After treatment, the levels of АШ, LAT, ODI, SLT90 % in the plasma group were significantly lower than those in the control group (P<0.05), and the LSa02 level was significantly higher than the control group (P<0.05). Conclusion: Low-temperature plasma radiofrequency ablation can significantly relieve the clinical symptoms of children, improve the body's ventilatory function and reduce the inflammatory response. [ABSTRACT FROM AUTHOR]

Objective: To investigate the expression of triggering receptor expressed on myeloid cells-1 (TREM-1) in lung tissue of rats with acute lung injury (ALI) induced by lipopolysaccharide and its relationship with endoplasmic reticulum stress and inflammatory response. Methods: 100 adult male SD rats were selected as research subjects, and they were divided into ALI group (n=60), control 1 groups (n=15), observation group (n=20) and control 2 group (n=5). The expression of TREM-1 and endoplasmic reticulum stress, the level of tumor necrosis factor-α (TNF-α) and the score of Smith were compared between the ALI group and control 1 group. The expres­sion of CHOP mRNA and GRP mRNA in the observation group and the control 2 groups were compared. The correlation between TREM-1 and endoplasmic reticulum stress, inflammatory response and Smith score was analyzed. Results: The levels of TREM-1 mRNA, CHOP mRNA, GRP78 mRNA, TREM-1,TNF-α and Smith scores in the ALI group at 6 h, 12 h, 1 d, 2 d were significantly higher than those in the control 1 group (P<0.05), and TREM-1 mRNA, CHOP mRNA, GRP78 mRNA,TREM-1,TNF-α and Smith scores in the ALI group were increased with the time, which were reached the highest level at 1 d, then there had a downward trend (P<0.05). The expres­sion of CHOP mRNA and GRPmRNA in the observation group at 6 h, 12 h, 1 d and 2 d was higher than that of the control 2 group, the expression of CHOP mRNA and GRP mRNA in the observation group increased with the time, which were reached the highest level at 1 d, then there had a downward trend (P<0.05). According to the correlation analysis of Spearman method showed that TREM-1 mRNA and TREM-1 levels were positively correlated with CHOP mRNA, GRP78 mRNA and Smith score, and TREM-1 level was positively correlated with TNF-α (P<0.05). Conclusion: The expression of TREM-1 is higher in the lung tissue of rats with ALI induced by lipopolysaccharide, and it is involved in the inflammatory response of the ALT lung. Activation of TREM-1 can enhance the endoplasmic reticulum stress of macrophages. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Fasudil combined with Seretide on the pulmonary function and serum levels of serum brain natriuretic peptide (BNP), C reactive protein (CRP), interleukin-8 (IL-8), and tumor necrosis factor-α (TNF-α) of patients with chronic obstructive pulmonary’ disease (COPD) complicated with pulmonary arterial hypertension (PAH). Methods: 108 cases of COPD combined with PAH patients treated in our hospital from February’ 2015 to January’ 2017 were selected and numbered according to the admission order and then they were divided into the observation group (n=54) and the control group with the method of random number table (n=54). Both groups were given conventional treatment, and the observation group was combined with Fasudil and Seretide on the basis of conventional treatment. Then the curative effect of the two groups of patients was evaluated. The lung function, blood gas analysis index, and serum BNP, CRP, IL-8 and TNF-α levels were compared between two groups before and after the treatment. Results: After the treatment, the FEVl%pre, FEV1/FVC and Pa02 of patients in both groups were significantly higher than those before the treatment(P<0.05), which were obviously higher in the observation group than those of the control group(P<0.05). The PaCO2 SPAP, Tei index serum BNP, CRP, IL-8, TNF-α levels of both groups were significantly higher than those before the treatment (P<0.05). which were significantly lower in the observation group than those of the control group (P<0.05). Conclusion: Fasudil combined with Seretide can effectively improve the pulmonary’ function, relieve the inflammation and reduce the pulmonary’ artery’ pressure in the treatment of COPD combined with PAH. [ABSTRACT FROM AUTHOR]

Objective: To investigate the clinical effect of octreotide-assisted duodenoscopy on the acute edematous biliary pancreatitis. Method: 100 patients with acute edematous biliary pancreatitis were selected and randomly divided into the study group and the control group with 50 cases in each group. Both groups of patients were given fasting, anti infection, nutritional support, enzyme inhibitor therapy, the control group was treated with duodenal endoscopy and the study group was treated with octreotide-assisted duodenoscopy at the same time. The abdominal pain relief time, white blood cells (WBC), temperature recovery time, hemodlastase and urine amylase, changes of serum CRP, IL-6, TNF-αlevels before treatment (T0), on the 3rd day after treatment (T1), on the 7th day after treatment(T2) were compared between the two groups. Results: ① The relief time of abdominal pain, WBC, body temperature returned to normal time of study group were all significantly shorter than those of the control group (P<0.05). ②The hemodlastase and urine amylase level at T1, T2 of both groups of patients were significantly lower than those at T0 and hemodlastase and urine amylase level at T2 lower than T1 (P＜0.05). The hemodlastase and urine amylase levels of study group were lower than those of the control group at T1, T2(P＜0.05). ③ The serum CRP, IL-6, TNF-αlevels in both groups at T1, T2 were significantly lower than at T0 with and T2 lower than T1 (P＜0.05), the serum CRP, IL-6, TNF-α levels of study group were lower than those of the control group at T1, T2 (P＜0.05). Conclusion: Octreotide- assisted duodenoscopy could effectively enhance the early clinical efficacy and relieve the inflammatory response of acute edematous biliary pancreatitis patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of ulinastatin (ULi) combined with alanyl glutamine (Ala-Gln) on inflammatory reactions and pulmonary functions in patients with cardiopulmonary bypass (CPB) valve replacement surgery. Methods: 54 cases who had taken the CPB valve replacement surgery in our hospital from June 2013 to November 2016 were selected and randomly divided into two groups. The patients in the control group were treated with conventional method, while the patients in the observation group were treated with Ala-Gln and ULi. Then the serum levels of inflammatory factors and the pulmonary surfactant protein A (SP-A), the lung function indexed and mechanical ventilation time between the two groups were observed and compared. Results: Compared with T0 the serum levels of IL-6, IL-8, TNF-α and SP-A in the two groups at T2，T3, T4 and T5 increased (P<0.01); Compared with the control group at the same time, the serum inflammatory factors and SP-A levels at T2，T3，T4 and T5 in the observation group were lower (P<0.01); Compared with T0, the RI and A-aDO2 in the two groups at T2，T3，T4 and T5 increased (P<0.01); Compared with the control group at the same time, the RI and A-aDO2 values at T2, T3, T4 and T5 in the observation group were lower (P<0.01); Compared with T0, the Cst in the two groups at T1 increased (P<0.01); The mechanical ventilation time in the observation group was significantly lower than that of the control group (P<0.01). Conclusion: ULi combined with Ala-Gln intervention on CPB valve replacement patients can effectively control the inflammatory reactions and protect the pulmonary functions, which is worthy of clinical application. [ABSTRACT FROM AUTHOR]

Objective: To study the effect of CO2 pneumoperitoneum on postoperative inflammatory reaction and immune response of elderly patients with acute appendicitis, and to provide basis for clinical treatment. Methods: 130 elderly patients with acute appendicitis who were treated in our hospital from January 2015 to March 2016 were selected, according to the random number table method,they were divided into study group and control group,with 65 patients in each group. The patients in control group were treated with conventional open appendectomy, while the patients in study group were treated with CO2 pneumoperitoneum laparoscopic appendectomy. The white blood cell count, neutrophil count, interleukin -10 (IL-10) and tumor necrosis factor -α (TNF-α ) level were compared between the two groups before and after operation 1 d, 3 d and 5 d. The white blood cell count, neutrophil count, levels of IL-10 and TNF-α in the two groups were significantly increased at 1 d after operation, the differences were statistically significant(P<0.05). Results: The white blood cell count and neutrophil count were compared with the control group at 1 d after operation, the differences were not statistically significant (P>0.05). The levels of TNF-α and IL-10 in the study group were significantly lower than that in the control group at 1 d after operation, the differences were statistically significant (P<0.05); 3 d, 5 d after operation compared with 1 d, the white blood cell count, neutrophil count, levels of IL-10 and TNF-α levels in the two groups were significantly reduced, there was no significant difference between the 3 d after operation and before operation (P>0.05), the difference between the 5 d after operation and before operation was statistically significant(P<0.05). The white blood cell count, neutrophil count, IL-10 and TNF-α in the study group were significantly lower than those in the control group at 3 d and 5 d after operation, the differences were statistically significant(P<0.05). Conclusion: CO2 pneumoperitoneum can effectively reduce postoperative inflammatory reaction and immune response of elderly patients with acute appendicitis, and improve the prognosis of patients. [ABSTRACT FROM AUTHOR]

The cardiovascular disease is one of the main causes of the humans death, and atherosclerosis, of which the inflammation reaction being an important pathological mechanism, is the pathological basis of it. Dyslipidemia, an important cause of inflammatory reaction, is an independent risk factor for atherosclerosis, occupying the whole progress. DNA methylation is one important way of epigenetics without changing the sequences of nucleotide and can regulate the genes expressions. Studies had shown, that the occurrence and development of dyslipidemia have close relationship with DNA methylation. The relationship between dyslipidemia and DNA methylation in the atherosclerosis fromthe dyslipidemia-related genes was reviewed in this article. [ABSTRACT FROM AUTHOR]

Objective: Through the observation of allicin preconditioning, observing the expression on cerebral ischemia-reperfusion in ICAM-1 area of hippocampus in rats, to study the mechanism of cerebral protection of allicin. Methods: 30 male Wistar rats, were randomly divided into 5 groups: sham operation group, ischemia reperfusion group, ischemia reperfusion+garlicin 10, 20, 30 mg/kg group. By four vessel occlusion methods for the preparation of rats with global cerebral ischemia reperfusion model in 24 h after reperfusion, removing the hippocampus, thionine staining to observe morphological changes of hippocampal tissue, Immunohistochemical staining was measured hippocampal CA1 area of ICAM-1 immunoreactivity and integral optical density of positive cells. Results: By giving complete cerebral ischemia 8min reperfusion in rats 24 h, morphology of hippocampal CA1 area organization changed significantly, neuron density decreased significantly; the expression of ICAM-1 increased significantly. Intravenous administration of allicin can make ischemia and morphology of hippocampal tissue perfusion changes significantly improved, the number of survival neurons increased, the expression of ICAM-1 was significantly less. Conclusion: Allicin can reduce ICAM-1 by inhibiting the expression and play a role in brain protection after cerebral ischemia - reperfusion inflammation loss. [ABSTRACT FROM AUTHOR]