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Lemon (Citrus limon) cultivars (Desi and Chinese) were subjected to gamma radiations for mutation induction. The current investigation was performed with the objective of exploring the effect of gamma irradiation on morphological characteristics of lemon and for development of lemon mutants for the sake of crop improvement. Seeds and bud wood of two lemon cultivars were exposed to various gamma ray concentrations (i.e 20,50,80,110 and 140 Gy). The irradiated buds were then budded on a rootstock (rough lemon) at standard growth stage of the root stock and seeds were sown in sterilized growing media in order to get disease free plants with maximum germination. The experimentation was carried out according to Randomized Complete Block Design (RCBD). The recorded observations were arranged and analyzed using statistical software Statistix 8.1 for employing statistical techniques. The research findings of the current investigation showed that there was significant variation in morphological variables among the irradiated and control plants of both cultivars. With the increase in radiation intensity, there was marked reduction in growth variables of both lemon varieties. [ABSTRACT FROM AUTHOR]