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This experiment is combined with the project of National Natural Science Foundation of innovation team in College of Tianjin City, to update the biotechnology and related professional’s comprehensive experimental project as the breakthrough point, to the primer of CRISPR locus in the genome of Arthrospira sp. Taking TJSD091 as an example, the paper elaborates how to determine the optimal annealing temperature in PCR amplification reaction by the combination of Touchdown PCR and gradient PCR. The reform stimulates students’ inquiry psychology and study interests, guides students to take the initiative to learn. The advantages of combining scientific research with experimental teaching are analyzed, it is believed that this teaching mode can train students’ scientific thinking, improve students' ability to solve practical problems, and can provide a new way for the improvement of the practical teaching system for the undergraduates in applied colleges. [ABSTRACT FROM AUTHOR]