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In the present work seeks to address the role of nanoencapsulated platform of Coenzyme Q10 (coQNPs) or selenium (SeNPs) in hyperlipidemia and alleviation of simvastatin-induced myopathy and adverse reactions. Physicochemical characters and zeta potential studies for solid nanoparticles (SLNPs), coQNPs and SeNPs were performed. Male Wistar albino rats were treated for 112 days with either (i) nano-vehicle only (ii) high fat diet (HFD) (iii) HFD + SV (20 mg/kg/day, P.O.) alone or (iv) with coQNPs (10mg/Kg/day, P.O.) or (v) / and (vi) SeNPs (0.1 mg/Kg/day, P.O.) for last 30 days. CoQNPs and SeNPs were shown in a uniform spherical shape with EE% 91.20 ±2.14, 94.89 ±1.54, respectively. HFD resulted in severe changes in all tested parameters that affirmed with histopathological results. Treatment with SV and CoQNPs/ or SeNPs exhibited a prominent restoring of all measured patterns, but the triple combination of SV, CoQNPs, and SeNPs exhibited an intense hepatic and muscular injury. SV and SeNPs treatment showed a pronounced improvement like control itself. These results represent that CoQNPs or SeNPs platforms showed advanced alleviation of hyperlipidemia, insulin resistance, with a direct limitation for its adverse effects such as myopathy and hepatotoxicity.

Osteoarthritis (OA) is a complex disease characterized by structural, functional, and metabolic deteriorations of the whole joint and periarticular tissues. In the current study, we aimed to investigate the possible effects of tempol on knee OA induced by the chemical chondrotoxic monosodium iodoacetate (MIA) which closely mimics both the pain and structural changes associated with human OA. Rats were administrated oral tempol (100 mg/kg) one week post-MIA injection (3 mg/ 50 μL saline) at the right knee joints for 21 consecutive days. Tempol improved motor performance and debilitated the MIA-related radiological and histological alterations. Besides, it subsided the knee joint swelling. Tempol decreased the cartilage degradation-related biomarkers as matrix metalloproteinase-13, cartilage oligomeric matrix protein, and fibulin-3. The superoxide dismutase mimetic effect of tempol was accompanied by decreased NADPH oxidase 4 (NOX4), inflammatory mediators, nuclear factor kappa-B (NF-κB), over-released insulin-like growth factor-1 (IGF-1) and transforming growth factor-β1 (TGF-β1). Tempol decreased the expression of chemotactic cytokine ligand 2 (CCL2), dickkopf‑related protein-1 (DKK-1), and protein kinase C (PKC). On the molecular level, tempol reduced the phosphorylated protein levels of p38 mitogen-activated protein kinase (MAPK), phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), and small mother against decapentaplegic 3 homologs (SMAD3). These findings suggest the promising role of tempol in ameliorating MIA-induced knee OA in rats via collateral suppression of the catabolic signaling cascades including TGF-β1/SMAD3/NOX4, NOX4/p38MAPK/NF-κB, and PI3K/Akt/NF-κB and therefore modulation of oxidative stress, catabolic inflammatory cascades, chondrocyte metabolic homeostasis, autophagy, and cell survival.