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Tubb4b (tubulin beta-4b chain) is essential for spermatogonia cell growth and development as a microtubule network protein, and its regulatory mechanism remains to be studied. To deeply understand the role of Tubb4b in spermatogonia cell proliferation and the cell cycle, we studied the role of spermatogonia in the control and TUBB4B-KO groups in different cell cycle phases by flow cytometry. We detected the crucial protein CDK2 in each stage by qRT–PCR and Western blotting. The results showed that under the induction of serum starvation, thymidine, and nocodazole under different conditions, mouse spermatogonia remained in the G0-G1 phase, S phase, and G2-M phase, respectively, and the change in the spermatogonia cycle in the Tubb4b-KO group was slower than that in the control group. In the study of cycle synchronization, it was found that the mRNA level of Cdk2 in the G2-M phase increased significantly (P Tubb4b-KO group, the mRNA level of Cdk2 increased significantly in the G0-G1 phase (P

To explore the role of jasmonic acid in seed germination of rice under Cd stress, the growth indices of JJ818 and NJ9108 under the bud stage were measured for identifying study the molecular mechanism of variety difference in cadmium tolerance. The tolerant variety JJ818 and the sensitive variety NJ9108 were treated under 150 µmol·L− 1 Cd concentration, methyl jasmonate (MeJA), and Ibuprofen (IBU) alone or in combination. The germination rate, root length, bud length, soluble protein content, soluble sugar content, and sugar components, α-amylase activity, and its related gene expression were measured. The results showed that the root length of the two rice materials were significantly inhibited during germination under the Cd treatment, however, the germination rate and shoot length had little affected. MeJA decreased the germination rate, root length and shoot length, and the contents of soluble sugar and sugar components and α-amylase activity as well. However, IBU treatment showed the opposite trend. Cd treatment also decreased the expression of the αAmy and RL1, and the accompanying changes of up-regulated the gene expression of JIOsPR10, OsLOX1, and OsLOX4 related to synthesizing jasmonic acid(JA). The results showed that JA negatively regulates the cadmium of rice and the genes related to the synthesis and response of JA were related to the variety difference in cadmium tolerance at the bud stage. Compared with the NJ9108, JJ818 was insensitive to jasmonic acid.