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The article reviews empirical studies on trends in attitudes toward lesbians and gay men and compares data from a 1985 survey of 297 students living in a college residence hall with data collected in 2001 when 152 students living in the same residence hall responded to a similar questionnaire. College students in 2001 expressed more accepting attitudes toward lesbians, reported more contact with lesbians, and assessed their parents' attitudes as more positive when compared with the 1985 sample. Consistent trends in the importance of these variables to the development of attitudes toward lesbians were shown, with gender role attitudes, parental attitudes, and exposure to education and media about gays and lesbians remaining the most important predictors. This suggests that strategies that discourage gender role stereotypes and provide educational and media experiences could increase acceptance of sexual minorities. Although gender was significant in 1985, there were no effects of gender in the 2001 sample. Continued study of trends in attitudes toward all sexual minorities and attention to the relative importance of gender and gender roles are recommended.