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Herein, we presented an enantioselective intramolecular Diels-Alder (IMDA) reaction with vinyl branched vinylidene ortho -quinone methide (VQM). The control of site selectivity in the IMDA reaction led to both chiral bridged bicyclo[4.3.1] and [5.3.1] architectures with high isolated yields (up to 85%) and excellent enantioselectivities (up to 97% ee).

The asymmetric synthesis of thiophene-derived compounds, including catalytic asymmetric dearomatization of thiophene and atroposelective synthesis of benzothiophene derivatives, has rarely been reported. In this work, the asymmetric transformation of the thiophene motif is investigated. Through the rational design of substrates with a thiophene structure, by using the vinylidene ortho -quinone methide (VQM) intermediate as a versatile tool, axially chiral naphthyl-benzothiophene derivatives and thiophene-dearomatized chiral spiranes were obtained in high yields with excellent enantioselectivities., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Sulfur compounds (SO 2 , CS 2 , H 2 S and OCS) are common toxic pollutants in the atmospheric environment, and the absorption spectroscopy technique can indeed help to realize online monitoring of their concentrations. However, nonlinear effects that occur during absorption spectroscopy measurements have a serious impact on the measurement of the absorption cross-sections (ACSs) of sulfur compounds, leading to serious deviations in both the substance absorption properties and concentrations obtained based on ACS analysis. In this paper, the maximum effective ACSs of sulfur compounds in the linear region are obtained by considering the influence of nonlinear effects and eliminating interference factors such as oxygen and photolysis. In addition, the nonlinear effects are found to be greatly attenuated in spectra with broad band absorption characteristics by comparing the oscillatory absorption spectra before and after the differential treatment and by comparing the change in the oscillatory ACS with the broad band ACS. The experimental results show that the effective ACSs of SO 2 , CS 2 , H 2 S, and OCS with a resolution of 0.23 nm are 14.15 × 10 -18 cm 2 per molecule, 5.61 × 10 -16 cm 2 per molecule, 7.09 × 10 -18 cm 2 per molecule, and 3.20 × 10 -19 cm 2 per molecule, respectively. So far, it is the largest ACS obtained at room temperature and atmospheric pressure, which is of great significance for online measurement of sulfur compounds.

Polymeric membrane ion-selective electrodes (ISEs) have been widely used in environmental monitoring. However, in complicated marine environments, marine biofouling usually becomes a sticky problem for these electrodes. Herein, for the first time, a novel maintenance-free antifouling potentiometric marine sensor based on a self-polishing coating (SPC) is proposed. The SPC is synthesized by using the seeded emulsion polymerization method based on the triisopropylsilyl methacrylate monomer as the regulator of the self-renewal rate. This coating can be simply modified onto the electrode surface by drop-casting. The silyl acrylate side groups of the obtained SPC on the sensor surface can be hydrolyzed in the marine alkaline medium. The shear movement of seawater driven by sea waves, wind, gravity, or vibration removes the leftover (fouled) brittle polymer backbone and thus the fouling marine microorganisms. As a proof-of-concept experiment, a polymeric membrane Ca 2+ -ISE is chosen as a model. Compared to the unmodified electrode, the SPC-coated Ca 2+ -ISE exhibits remarkable improved antifouling properties in terms of superior anti-adhesive abilities towards marine microorganisms, such as bacterial cells and algae and excellent long-term stability even in the presence of high levels of marine microorganisms. Since no additional manual maintenance is required for maintaining the antifouling abilities of the sensor, the proposed self-polishing sensor may lay an important foundation for construction of unattended long-term potentiometric monitoring systems in real marine environments.

Postprandial hyperglycemia is an independent risk factor for cardiovascular diseases, and the impact of tea polyphenols (TP) and rutin, representative phenolic compounds with different water solubilities, on the postprandial glycemic response to cooked normal corn starch (CCS) was investigated. Comparatively, TP (DPPH 50 = 0.12 mmol L -1 ) are more potent than rutin (DPPH 50 = 0.50 mmol L -1 ) in scavenging the free radicals of DPPH, but both TP and rutin inhibited the activity of porcine pancreatic α-amylase (PPA), the major enzyme in starch digestion, with an IC 50 of 4.09 mmol L -1 and 2.71 mmol L -1 , respectively. However, an in vivo study showed that a significant reduction in postprandial blood glucose was only observed in the presence of rutin, and TP had no effect on the glycemic response to CCS. To find out the underlying mechanism, fluorescence spectroscopy and molecular docking were carried out and they showed that, compared to TP, rutin bound to the active site of PPA with higher affinity and a lower free energy (Δ G ) driven by hydrogen bonds and π-stacking, and rutin also greatly increased the viscosity of starch. Collectively, water-soluble TP have a higher antioxidant property and a lower potency to inhibit PPA compared to water-insoluble rutin, and the weaker interaction between TP and PPA, and starch as well might synergistically contribute to TP's ineffectiveness in lowering the postprandial glycemic response, and water solubility linking the molecular structures and functions of phenolic compounds might be the fundamental basis for the observed difference in their biological functions, and water solubility can also be used to enrich specific phenolic compounds for desired functions.

Carrier-based polymeric membrane potentiometric sensors are an ideal tool for detecting ionic species. However, in the fabrication of these sensors, the screening of carriers still relies on empirical trial- and error-based optimization, which requires tedious and time-consuming experimental verification. In this work, computer-aided screening of carriers is applied in the preparation of polymeric membrane potentiometric sensors. Molecular docking is used to study the host-guest interactions between receptors and targets. Binding energies are employed as the standard to screen the appropriate carrier. As a proof-of-concept experiment, the antibiotic ciprofloxacin is selected as the target model. A series of supramolecular macrocyclic receptors including cyclodextrins, cucurbiturils and calixarenes are chosen as potential receptors. The proposed sensor based on the receptor calix[4]arene screened by molecular docking shows a lower detection limit of 0.5 μmol L -1 for ciprofloxacin. It can be expected that the proposed computer-aided screening technique of carriers can provide a simple but highly efficient method for the fabrication of carrier-based electrochemical and optical sensors.

Tungsten coatings have unique properties such as high melting points and hardness and are widely used in the nuclear fusion and aviation fields. In experiments, compared to pure Na 2 WO 4 molten salt, electrolysis with Na 2 WO 4 -WO 3 molten salt results in a lower deposition voltage. Herein, an investigation combining experimental and computational approaches was conducted, involving molecular dynamics simulations with deep learning, high-temperature in situ Raman spectroscopy and activation strain model analysis. The results indicated that the molten salt system's behaviour, influenced by migration and polarization effects, led to increased formation of Na 2 W 2 O 7 in the Na 2 WO 4 -WO 3 molten salt, which has a lower decomposition voltage and subsequently accelerated the cathodic deposition of tungsten. We analyzed the mechanism of the effect of the electric field on the Na 2 W 2 O 7 structure based on the bond strength and electron density. This research provides crucial theoretical support for the effect of electric field on tungsten in molten salt and demonstrates the feasibility of using machine learning-based DPMD methods in simulating tungsten-containing molten salt systems.

Based on the excellent adsorption properties of carbon materials, a new magnetic nanodemulsifier was prepared in this study. First, carbon nanotubes were oxidized using a solvothermal method. Then, Fe 3 O 4 was combined with oxidized carbon nanotubes using a one-pot method, and then grafted onto fluorine-containing polyether to prepare a magnetic composite demulsifier (Fe 3 O 4 @C-F) with good demulsification properties. The surface morphology of the composite demulsifier was analyzed using scanning electron microscopy (SEM). The structure of the composite demulsifier was characterized using Fourier transform infrared (FTIR) spectroscopy and X-ray photoelectron spectrometry (XPS). The stability of the composite demulsifier was characterized using thermogravimetric analysis (TGA). Results showed that the oxidized carbon nanotubes and fluorinated polyether were successfully attached to Fe 3 O 4 . The experimental objective was to obtain a self-made crude oil emulsion. The demulsification test and recovery performance test were then performed, and the main factors affecting the demulsification performance of the demulsifier were investigated. Results showed that when the dosage was 800 mg L -1 , the temperature was 65 °C, the demulsification time was 90 min, and the pH value was 6. The demulsification effect of the Fe 3 O 4 @C-F magnetic composite demulsifier was the best, whereby the demulsification rate could reach 91.68%, and the oil-water interface was clear. Fe 3 O 4 @C-F had a magnetic response and could be recycled from the two-phase system six times under the action of an external magnetic field. Fe 3 O 4 @C-F is an efficient and environmentally friendly demulsifier that has important application value for enriching demulsification technology systems., Competing Interests: The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (This journal is © The Royal Society of Chemistry.)

Antibiotics in aquatic environments present a serious threat to the ecological environment and human health. Activation of carbon-catalyzed persulfate is a prospective approach for oxidizing antibiotics. There is a pressing need for inexpensive carbon catalysts of high quality. In this study, biochar (BC) modified by Fe, Mn and Fe@Mn was employed to activate peroxymonosulfate (PMS) to degrade carbamazepine (CBZ) in water. The surface of Fe@Mn BC had a dense, stalactite-like morphology comprising a square chassis that was elliptical. The catalyst Fe@Mn-BC possessed the optimal degradation effect (99%) on CBZ at 100 min. Electron paramagnetic resonance spectroscopy and the quenching spectrum suggested that ˙O 2 - and 1 O 2 contributed to CBZ degradation., Competing Interests: The authors declare no conflicts of interest., (This journal is © The Royal Society of Chemistry.)

We propose here a pulsed galvanostatic control of a solid-contact ion-selective electrode coupled with a thin-layer ion-exchanger free membrane, which allows chronopotentiometric trace-level ion detection with a high-interfering background in a rapid and reversible way.

Due to its intriguing features and numerous applications, graphene has garnered a lot of interest in recent years. However, it is still very difficult to create graphene-based room-temperature magnets without transition metals or rare earth elements since pristine graphene is inherently diamagnetic due to the delocalized π bonding network. Herein, room-temperature ferromagnetism with a saturation magnetization of 0.93 emu g -1 (300 K) is achieved in defect-rich-reduced graphene oxide (DR-rGO) nanoscrolls by creating a spatial coupling of defects. The experiments and DFT calculations verify that spatial coupling of defects could enhance Rudermann-Kittel-Kasuya-Yosida interactions to induce magnetism in graphene. It displays high-efficiency electromagnetic wave absorption performance with a minimal reflection loss of -62.1 dB and an effective absorption bandwidth of 7.8 GHz (3.0 mm) thanks to greatly improved magnetism. This breakthrough serves as a building block for the creation of room-temperature magnetic carbon materials and expands their applications in many pertinent domains.

Pre-stretching as a method for directing polymer crystallization offers a promising solution for addressing the limitations of solid polymer electrolytes in flexible batteries at ambient temperatures. In this study, we have investigated the ionic conductivity, mechanical behaviour, and microstructural and thermal properties of polyethylene oxide (PEO)-based polymer electrolytes with varying pre-strain levels. The results indicate that thermal stretching-induced pre-deformation can significantly increase the through-plane ionic conductivity, in-plane strength, stiffness of solid electrolytes, and cell-specific capacity. However, modulus and hardness decrease for pre-stretched films in the thickness direction. Notably, applying 50-80% pre-strain to the PEO matrix composites through thermal stretching may be preferred for improving the electrochemical cycling performance, as it can increase through-plane ionic conductivity by at least 1.6 times while maintaining compressive stiffness at 80% compared to their unstretched counterparts, while the in-plane strength and stiffness can be boosted by 120-140%. Besides, adding nanoceramics contributes to lithiated PEO exhibiting a higher enhancement coefficient than the pristine sample. This positive effect is because the pre-strain and nano-inorganic filler decrease crystallinity and increase the free volume size of pre-stretched PEO-based electrolytes.

Herein, a metal-free main-group catalysis system for thiosulfonylation of terminal alkynes with thiosulfonates has been established by using commercially available B(C 6 F 5 ) 3 as a catalyst. The protocol provides a highly regio- and stereoselective route for the synthesis of diverse ( E )-(β)-arylthiolvinyl sulfones under mild conditions with 100% atom-economy and exceptional functional group compatibility.

Covalent organic frameworks (COFs) for detecting biological macromolecules in water or biological environments are generally challenging. In this work, a composite material IEP-MnO 2 is obtained by combining manganese dioxide (MnO 2 ) nanocrystals and a fluorescent COF (IEP), which is synthesized by using 2,4,6-tris(4-aminophenyl)- s -triazine and 2,5-dimethoxyterephthalaldehyde. By the addition of biothiols, such as glutathione, cysteine or homocysteine with different sizes, the fluorescence emission spectra of IEP-MnO 2 changed ("turn-on" or "turn-off") via different mechanisms. The fluorescence emission of IEP-MnO 2 increased in the presence of GSH by the elimination of the FRET (Förster resonance energy transfer) effect between MnO 2 and IEP. Surprisingly, due to the formation of a hydrogen bond between Cys/Hcy and IEP, the fluorescence quenching for IEP-MnO 2 + Cys/Hcy may be explained via the photoelectron transfer (PET) process, which endows IEP-MnO 2 with specificity in distinguishing the detection of GSH and Cys/Hcy compared to other MnO 2 complex materials. Therefore, IEP-MnO 2 was used to detect GSH and Cys in human whole blood and serum, respectively. The limit of detection for GSH in whole blood and Cys in human serum was calculated to be 25.58 μM and 4.43 μM, which indicates that IEP-MnO 2 can be used to investigate some diseases related to GSH and Cys concentration. Moreover, the research expands the application of covalent organic frameworks in the fluorescence sensing field., Competing Interests: We declare that there is no conflict of interest., (This journal is © The Royal Society of Chemistry.)

We report the self-assembly of amphiphilic BDQ photosensitizers into lysosome-targeting nanophotosensitizer BDQ-NP for highly effective photodynamic therapy (PDT). Molecular dynamics simulation, live cell imaging, and subcellular colocalization studies showed that BDQ strongly incorporated into lysosome lipid bilayers to cause continuous lysosomal membrane permeabilization. Upon light irradiation, the BDQ-NP generated a high level of reactive oxygen species to disrupt lysosomal and mitochondrial functions, leading to exceptionally high cytotoxicity. The intravenously injected BDQ-NP accumulated in tumours to achieve excellent PDT efficacy on subcutaneous colorectal and orthotopic breast tumor models without causing systemic toxicity. BDQ-NP-mediated PDT also prevented metastasis of breast tumors to the lungs. This work shows that self-assembled nanoparticles from amphiphilic and organelle-specific photosensitizers provide an excellent strategy to enhance PDT., Competing Interests: The authors declare no competing interest., (This journal is © The Royal Society of Chemistry.)

The effect of tea polyphenols (TPL) on the gluten network structure in TPL-fortified bread and dough systems was investigated. In the bread system, the addition of tea polyphenols (TPL) dose-dependently (0, 0.5, 1.0, 1.5, and 2%) deteriorated the bread quality in terms of the loaf volume, hardness and sensory properties. In the gluten dough system (2% TPL), farinograph results showed that the stability and development time of the bread dough were decreased, resulting in a weak dough with a significantly increased elastic modulus ( G '). The disulfide bonds, key to the dough network formation, were decreased by 9.9 μmol g -1 (24.2%), and their stability was also reduced due to the reduction (15.0%) of the relative content of the gauche - gauche - gauche structure. The addition of tea polyphenols also increased the internal hydrogen bonds and hydrophobic forces along with the increased random coil and decreased α-helix secondary structure of gluten. The water distribution experiment showed that the bound water peak in the nuclear magnetic resonance (NMR) spectrum disappeared, but the adsorbed water and free water were increased by 17.3% and 0.4%, respectively. Collectively, the interaction between wheat gluten and tea polyphenols affects the water-gluten relationship and the protein structure, which leads to a disrupted network structure of the wheat dough and deteriorated bread quality. Strategies to prevent gluten-phenolic interaction in functional bread preparation warrant further investigation.

Extracellular vesicles (EVs) are membranous vesicles released by cells that carry a number of biologically important components such as lipids, proteins, and mRNAs. EVs can mediate cancer cell migration, invasion, angiogenesis, and cell survival, greatly contributing to cell-to-cell communication in the tumor microenvironment. Additionally, EVs have been found to have diagnostic and prognostic significance in various cancers. However, the direct isolation of pure EVs remains challenging, especially from tissue samples. Currently available EV isolation approaches, e.g. , ultracentrifugation, are time-consuming, instrumental dependent, and have a low recovery rate with limited purity. It is urgent to develop rapid and efficient methods for enriching tissue EVs for biological and clinical studies. Here, we developed a novel isolation approach for tissue EVs using an extraction kit combined with TiO 2 microspheres (kit-TiO 2 ). The EVs were first precipitated from the tissue fluid using a precipitation agent and then further enriched using microspheres based on the specific interaction between TiO 2 and the phosphate groups on the lipid bilayer of the EVs. Kit-TiO 2 approach led to improved purity and enrichment efficiency of the isolated EVs, as demonstrated by western blot and proteomic analysis, compared with previously reported methods. A total of 1966 protein groups were identified from the tissue EVs. We compared the proteomic profiles of the liver tissue EVs from healthy and hepatocellular carcinoma (HCC) bearing-mice. Twenty-five significantly upregulated and 75 downregulated protein groups were found in the HCC EVs. Among the differentially expressed proteins, Atic, Copa, Cont3, Me1, Anxa3, Fth1, Anxa5, Phb1, Acaa2, ATPD, and Glud1 were reported to be highly relevant to HCC. This novel isolation strategy has provided a powerful tool for enriching EVs directly from tissues, and may be applied in biomarker discovery and drug screening of HCC., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Graphdiyne (GDY) is an sp and sp 2 co-hydrocarbon allotrope whose particular structure endows it with many fascinating properties, including abundant chemical bonds, high conjugation, natural pores, high carrier mobility, high conductivity and stability, etc . In this work, two-dimensional graphdiyne is prepared as an electrode substrate material coupling with an exonuclease III-assisted amplification strategy to construct a superior-performance self-powered biosensor based on enzymatic biofuel cells for highly sensitive detection of the tumour marker miRNA-21. Glucose oxidase (GOD) is first immobilized on the GDY/AuNP composite to prepare a bioconjugate. GDY/AuNP modified carbon cloth is used as an enzyme biofuel cell electrode, which is then modified with bilirubin oxidase as a biocathode. The bioconjugate binds to GOD through specific binding to the bioanode. When miRNA-21 is present, specific recognition by exonuclease III in the system results in cleavage of the capture probe, and miRNA-21 is recovered and involved in the cycle. The target miRNA-21 then causes corresponding changes in the open-circuit voltage of the self-powered system. Based on this, a sensitive detection method was constructed, within the scope from 0.1 fM to 0.1 nM with a shallow detection limit of 55.2 aM (S/N = 3). The new approach triumphantly has been used to detect miRNA-21 in serum, which provides a compelling new way for early diagnosis of related cancers.

Post-translational modifications (PTMs) play important roles in modulating the biological functions of proteins. Stoichiometry, which quantifies the modification percentage, is a critical factor for any given PTM. In this work, we developed a chemoproteomic strategy called "STO-MS" to systematically quantify the PTM stoichiometry in complex biological samples. This strategy employs a resolvable mass tag to differentiate proteoforms with different numbers of modifications and utilizes liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) techniques to measure PTM stoichiometry at the proteomic level. As a proof-of-concept, we successfully determined the stoichiometry of 197 proteins modified by 4-hydroxynonenal (HNE), a well-characterized lipid-derived electrophile and biomarker for oxidative stress. Our work expands the toolbox for quantification of PTM stoichiometry and sheds light on understanding the biological significance of PTMs in oxidative stress., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

With the increasing demand for rare earth elements (REEs) due to their wide application in high technology, their recovery and separation from waste sources has gradually come onto the agenda. Herein, a new kind of MIL-101-NH 2 (M1N) MOF functionalized with diethanol anhydride (DGA) incorporated into a polyvinylidene fluoride (PVDF) membrane (DGA-M1N@PVDF) has been fabricated for the sorption of REEs from a simulated acid leaching solution of waste phosphor, which contains a large amount of REEs. FTIR, TGA, XRD, fluorescence spectra and XPS analysis were used to characterize the synthesized composite membrane. Batch tests were employed to determine the optimal sorption conditions for Y and Eu adsorbed on DGA-M1N@PVDF adsorbent, such as pH (1-5), content of M1N MOFs (0-40 wt%), contact time (10-180 min) and ion concentration (0-20 mg L -1 ). Maximum adsorption capacities for Y and Eu on DGA-M1N@PVDF reached 991.7 μg g -1 and 98.76 μg g -1 for trace REE solution, respectively. Moreover, a pseudo-second-order kinetic model accurately described the sorption process, and the plotted isothermal data indicated that the Langmuir model was more suitable than the Freundlich model for Y and Eu sorption with monolayer and chemical adsorption. Meanwhile, FTIR and XPS analyses revealed that the Y and Eu adsorption on the DGA-M1N@PVDF composite membrane was mainly caused by the N and O atoms of the -CONH or -COOH groups coordinated with metal ions. Furthermore, after five cycles, the recovery efficiency by DGA-M1N@PVDF for REEs remains above 82% and the XRD patterns were consistent with the original sample, which implied that the DGA-M1N@PVDF membrane has preferable stability, recyclability and good efficiency in REE separation from waste phosphor solutions., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

We design and synthesize a novel 1,8-naphthalimide-based fluorescent probe MNP that features the dual capabilities of tracking lysosomes in living HeLa cells and sensitively detecting Fe 3+ ions in aqueous solution. The MNP is obtained by modifying the morpholine group with a lysosomal targeting function and the piperazine group with an Fe 3+ ion recognition function on the 1,8-naphthalimide matrix. In the presence of Fe 3+ ions, the MNP acts as a recognition ligand to coordinate with the central Fe 3+ ion, and the protonated [MNPH] + is eventually generated, in which significant fluorescence enhancements are observed due to the intramolecular photo-induced electron transfer (PET) process being blocked. The limit of detection of Fe 3+ ions is as low as 65.2 nM. A cell imaging experiment shows that the MNP has low cytotoxicity and excellent lysosomal targeting ability. Therefore, the MNP offers a promising tool for lysosomal tracking and relevant life process research., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Polyubiquitination signal deliver diverse cellular signal, which have been recognized as a sophisticated ubiquitin code. The perception and transduction of ubiquitination signal depend on the specificity and sensitivity of the ubiquitin-binding domain. Accurate and sensitive detection of polyubiquitination signal is crucial for revealing the dynamic cellular ubiquitin-regulated events. Western blotting (WB) and immunohistochemistry (IHC) are the most widely used biochemical strategies to detect ubiquitination signal on substrates under diverse physiological and pathological conditions. However, anti-ubiquitin antibodies fail to reflect polyubiquitination signal unbiasedly because of their strong preference for K63-linked ubiquitin chains. Herein, we demonstrated that our previously developed tandem hybrid ubiquitin-binding domain (ThUBD) chemically labeled with a reporter group such as horseradish peroxidase (ThUBD-HRP) could significantly improve the robustness and sensitivity of polyubiquitination signal detection. This advanced method was named TUF-WB Plus (TUF-WB + ). The TUF-WB + method significantly increases the sensitivity and accuracy of ubiquitin detection and requires a shorter experimental operation time. Furthermore, it enables the ThUBD-HRP probe to function as a powerful tool for spatial in situ polyubiquitination detection in cells by immunohistochemistry. Our newly developed ThUBD-HRP probe and TUF-WB + method provide a robust and powerful tool for ubiquitination signal detection with hypersensitivity in an unbiased manner.

Postoperative infection and poor osteogenesis will cause the failure of dental implant surgery. Thus, the antibacterial and osteogenic activities are the core requirements for the surface modification of dental implants. Inspired by the strong chelating ability of naturally occurring phytic acid (PA), an in situ deposition method on the surface of titanium implants was developed based on the metal-phosphate coordination networks. Biologically relevant metal cations ( i.e. ferric ions and divalent copper ions) were selected as metal constituents for the construction of organic-inorganic coordination network films. The stability of PA-metal coordination bonds is rationally explained by the chemical nature of transition metal elements. This PA-metal coordination complex coating exhibited an excellent antibacterial activity against Porphyromonas gingivalis , reducing the bacterial implant colonization by > 3.92 log 10 . The abundant phosphate groups greatly increased the surface hydrophilicity, promoted the early adhesion of proteins, improved the proliferation of bone marrow mesenchymal stem cells, and finally achieved an enhanced osteogenic activity. In addition, the phosphate groups of PA also facilitated the deposition of hydroxyapatite by providing reaction sites to chelate with calcium ions. These findings evaluate the anti-bacterial and osteogenic potentials of PA-metal coordination complexes, and clarify the feasibility for surface modification of dental implants.

As deep space exploration moves farther and farther away, thermal control coating of the in-orbit spacecraft will suffer a serious vacuum-ultraviolet radiation environment, which seriously threatens the reliability of the spacecraft in orbit. Therefore, it is important to improve the vacuum-ultraviolet resistance performance of the thermal control coating. In this work, the inorganic Al 2 O 3 -ZnO-Y 2 O 3 thermal control coating was in situ fabricated on a 6061 aluminum alloy surface by PEO technology, and its vacuum-ultraviolet resistance performance was investigated. The results show that the Al 2 O 3 -ZnO-Y 2 O 3 thermal control coating has a good resistance performance to vacuum-ultraviolet radiation, which is mainly because the large extinction coefficients of the ZnO and Y 2 O 3 materials in the ultraviolet band are conducive to improving the ultraviolet resistance performance. Furthermore, the life prediction model of the Al 2 O 3 -ZnO-Y 2 O 3 thermal control coating shows that its Δ α s value first slightly increases and then tends to be stable with the increase of ultraviolet irradiation time from 0 ESH to 25 000 ESH, and the maximum variation of Δ α s is about 0.0536. This work provides a material basis and technical support for the thermal control system of spacecraft with long life and high reliability., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Topological defects such as dislocations in crystalline materials usually have major impacts on materials' mechanical, chemical and physical properties. Detailed knowledge of dislocation core structures is essential to understand their impacts on materials' properties. However, compared with imaging of core structures of edge dislocations, direct imaging of a screw dislocation core is challenging from the traditional edge-on direction because the atomic displacements are parallel to the screw dislocation line. Here, a screw dislocation with a Burgers vector 1/2[110] in orthorhombic CsPbBr 3 nanocrystals is directly imaged at the atomic scale with the incident electron beam perpendicular to the dislocation line using aberration-corrected scanning transmission electron microscopy (STEM). The dislocation core is characterized by helical atomic planes along the dislocation line. Quantitative assessments of the change rate of the screw displacements reveal the dislocation line locate at a plane containing Cs and Br atoms. This study reveals the atomic structure of screw dislocation cores in CsPbBr 3 and provides useful information for the understanding of structure-property relations of halide perovskites.

Micrometer-resolution 3D printing of functional oxides is of growing importance for the fabrication of micro-electromechanical systems (MEMSs) with customized 3D geometries. Compared to conventional microfabrication methods, additive manufacturing presents new opportunities for the low-cost, energy-saving, high-precision, and rapid manufacturing of electronics with complex 3D architectures. Despite these promises, methods for printable oxide inks are often hampered by challenges in achieving the printing resolution required by today's MEMS electronics and integration capabilities with various other electronic components. Here, a novel, facile ink design strategy is presented to overcome these challenges. Specifically, we first prepare a high-solid loading (∼78 wt%) colloidal suspension that contains polyethyleneimine (PEI)-coated stannic dioxide (SnO 2 ) nanoparticles, followed by PEI desorption that is induced by nitric acid (HNO 3 ) titration to optimize the rheological properties of the printable inks. Our achieved ∼3-5 μm printing resolution is at least an order of magnitude higher than those of other printed oxide studies employing nanoparticle ink-based printing methods demonstrated previously. Finally, various SnO 2 structures were directly printed on a MEMS-based microelectrode for acetylene detection application. The gas sensitivity measurements reveal that the device performance is strongly dependent on the printed SnO 2 structures. Specifically, the 3D structured SnO 2 gas sensor exhibits the highest response of ∼ 29.9 to 100 ppm acetylene with the fastest total response time of ∼ 65.8 s. This work presents a general ink formulation and printing strategy for functional oxides, which further provides a pathway for the additive manufacturing of oxide-based MEMSs.

A PGP-1-specific one/two-photon fluorogenic probe (BH1), capable of high sensitivity, super selectivity, and visual imaging of endogenous PGP-1 activity from live mammalian cells and serum/skin tissues from patients by using one/two-photon fluorescence microscopy (O/TPFM).

β-Keto acids, ideal surrogates of inactive ketones, play an important role in organic synthesis. The asymmetric decarboxylative reaction using β-keto acids is the one which is being studied the most. Herein we present a comprehensive review on this research topic, which is generally classified according to different catalytic systems and chiral induction modes. Additionally, some extended utilities of these methodologies for synthesizing bioactive compounds were also summarized. This review will facilitate the synthetic community to understand the role of β-keto acids in asymmetric reactions, providing many new opportunities for further exploration in this field.

6,6'-Dibromided tert -butyloxycarbonyl isoindigo (Br-TBOCII) has intense fluorescence in the solid state via excitation with aggregation-induced emission (AIE), contrary to the classic heavy-atom effect. The unique AIE mechanism is attributed to the Br-Br bonding joint restricting intramolecular motion. Furthermore, the water-soluble nanoparticles Br-TBOCII/Pluronic® 127, possess robust photostability, low toxicity and good cell imaging performance.

Staining of biological cells with heavy metals can increase their visibility in mass spectrometry. In this study, the potential of ruthenium red (RR) as a staining agent for single-cell analysis by inductively coupled plasma time-of-flight mass spectrometry (SC-ICP-TOF-MS) is explored using two different yeast strains and one algal species. Time-of-flight mass spectrometry allows the simultaneous detection of Ru and multiple intrinsic elements in single cells. Ru has a better correlation with Mg than with P in Saccharomyces cerevisiae ( S. cerevisiae ) cells. For the three tested strains, the staining efficiency of RR exceeded 96%; the staining strengths were 30-32 ag μm -2 for the yeast cells and 59 ag μm -2 for the algal cells. By deriving the cell volume of single cells from their Ru mass, the concentration of Mg and P in individual cells of S. cerevisiae can be calculated. Elemental concentrations of Mg and P were highly variable in the cell individuals, with their 25-75 percentile values of 0.10-0.19 and 0.76-2.07 fg μm -3 , respectively. RR staining has several advantages: it is fast, does not affect cell viability and is highly efficient. Provided that the shape of the individual cells of a culture is similar, Ru staining allows the elemental content to be directly correlated with the cell volume to accurately calculate the intracellular concentration of target elements in single cells. Therefore, RR can be a promising cell staining agent for future application in SC-ICP-TOF-MS research.

To overcome the urgency of zirconium and hafnium separation, a novel mesoporous silica sorbent (PS-G1.0-MSNs) modified with phosphorous-functionalized G1.0 PAMAM dendrimers was prepared. The adsorption and separation behaviors of PS-G1.0-MSNs adsorbent on Zr(iv) and Hf(iv) were perfromed as a function of acidity, contact time, temperature, and ion concentrations by batch sorption methods. The maximum adsorption capacities for Zr(iv) and Hf(iv) were 25.7 mg g -1 and 5.36 mg g -1 under optimal experimental conditions, respectively, and the separation factor β Hf/Zr = 2.0 > 1 demonstrated that the prepared sorbent had preferential selectivity for Hf(iv) in rich Zr(iv) solution. Moreover, kinetic data indicated that the sorption process on Zr(iv) and Hf(iv) achieved equilibrium within 120 min, and followed the pseudo-first-order model with a rate-determining step. The adsorption amount increased as temperature raised from 283 K to 303 K and the isothermal data plotted with the Langmuir model was better than the Freundlich model with monolayer behavior. Thermodynamic data analysis indicated that the sorption process was spontaneous and endothermic. Furthermore, XPS analysis revealed that the metal ion adsorption was mainly induced by the chemical coordination of Zr(iv) and Hf(iv) ions with N, O, P atoms of amide and phosphate groups. The present work provides good guidelines on the design of high efficient sorbent for the separation of Hf(iv) from Zr(iv) solutions., Competing Interests: All of the authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (This journal is © The Royal Society of Chemistry.)

An organocatalytic approach to installing various alcohols into the carbonyl of α,β-unsaturated ketones mediated by VQM intermediates was achieved, followed by dearomatization to provide the stereo-defined cyclic ethers via a cascade process. Along with the transformations, this strategy affords efficient access to the underexplored chiral cyclic ether chemospace.

The effects of the urban morphological characteristics on the spatial variation of near-surface PM 2.5 air quality were examined. Unlike previous studies, we performed the analyses in real urban environments using continuous observations covering the whole scale of urban densities typically found in cities. We included data from 31 measurement stations divided into 8 different wind sectors with individually defined morphological characteristics leading to highly varying urban characteristics. The urban morphological characteristics explained up to 73% of the variance in normalized PM 2.5 concentrations in street canyons, indicating that the spatial variation of the near-surface PM 2.5 air quality was mostly defined by the characteristics studied. The fraction of urban trees nearby the stations was found to be the most important urban morphological characteristic in explaining the PM 2.5 air quality, followed by the height-normalized roughness length as the second important parameter. An increase in the fraction of trees within 50 m of the stations from 25 percentile to 75 percentile ( i.e. by the interquartile range, IQR) increased the normalized PM 2.5 concentration by up to 24% in the street canyons. In open areas, an increase in the trees by the IQR actually decreased the normalized PM 2.5 by 6% during the pre-COVID period. An increase in the height-normalized roughness length by the IQR increased the normalized PM 2.5 by 9% in the street canyons. The results obtained in this study can help urban planners to identify the key urban characteristics affecting the near-surface PM 2.5 air quality and also help researchers to evaluate how representative the existing measurement stations are compared to other parts of the cities., Competing Interests: There are no conflicts of interest to declare., (This journal is © The Royal Society of Chemistry.)

Pyroglutamate aminopeptidase-1 (PGP-1) is an important enzyme that plays an indispensable role in the process of inflammation. Up to now, few reports have been reported on the detection of PGP-1 activity in vivo and in vitro, and there are no reports on ratiometric detection. Here, the first red-emitting ratiometric fluorescent sensor (DP-1) for the specific detection of PGP-1 both in vivo and in vitro was designed and synthesized by using DCD-NH2 as the luminescent parent and pyroglutamate as a recognition group. After interacting with PGP-1, the amide bond is hydrolyzed by the enzyme and the color of the solution changes from yellow (λabs = 420 nm) to red (λabs = 520 nm), accompanied by obvious fluorescence emission wavelength change (from ∼564 nm to ∼616 nm). The probe has high specificity and sensitivity towards PGP-1 in about 10 min, and the DL is as low as 0.25 ng mL-1. Interestingly, under the stimulation of Freund's incomplete adjuvant and lipopolysaccharide, the imaging of DP-1 in HepG2 and RAW264 cells shows that the expression of PGP-1 is associated with inflammation. What's more, for the first, the imaging of a mouse tumor model confirms that the enzyme is closely related to the occurrence of some inflammation and tumor diseases. These results indicate that DP-1 can be used as an effective tool for real-time monitoring of PGP-1 levels both in vivo and in vitro and the study of inflammatory tumor pathology.

During weaning transition, mammalian newborns suffer severe enteric infections and thus induced gut microbiota dysbiosis, which in turn aggravates enteric disorder. The synthetic dipeptide glycyl-glutamine (GlyGln) has been used as a diet supplement to improve the weaning transition of newborns. However, the effect of dietary GlyGln supplementation on the gut microbiota of piglets with enteric infection remains unclear. Here, weaned piglets received a basal diet or a basal diet supplemented with 0.25% GlyGln for 3 weeks. Five piglets in each group received an intraperitoneal injection of lipopolysaccharide (LPS) (100 μg per kg BW) (LPS and GlyGln + LPS groups) and meanwhile five piglets in a control group received an intraperitoneal injection of saline (Ctrl group). The results showed that dietary GlyGln supplementation improved the LPS induced inflammation response and damage to the ileum morphology by increasing interleukin 10, tight junction proteins, villus height, and the ratio villus height/crypt depth, but decreasing the crypt depth. For the oxidative status, dietary GlyGln supplementation increased the ileal superoxide dismutase and meanwhile reduced the malondialdehyde and nitric oxide synthase activity (NOS) (total NOS and inducible NOS), compared with that in the LPS group. LPS challenge reduced the diversity of gut microbiota and enriched the facultative anaerobic Escherichia coli. The GlyGln restored alpha diversity and the structure of the gut microbiota by enriching obligate anaerobes and short-chain fatty acid (SCFA)-producing bacteria, including Clostridium, Lachnospira, Phascolarctobacterium, Roseburia, Lachnospiraceae, and Synergistetes. GlyGln enriched the gut microbiota function of carbohydrate metabolism and elevated the ileal SCFA concentrations of propionic acid and butyric acid that had been decreased by the LPS challenge. The beneficial effects of dietary GlyGln supplementation are closely associated with its enriched bacteria and SCFAs. Taken together, dietary GlyGln supplementation improved the gut microbiota dysbiosis induced by LPS challenge and enriched obligate anaerobes and SCFA-producing bacteria, which contributed to the amelioration of intestinal integrity, inflammatory responses, and oxidative status.

Itaconate is an immunoregulatory and anti-bacterial metabolite, and plays important roles in host-pathogen interactions. Chemoproteomic strategies have been used to explore the anti-inflammatory effects of itaconate on activated macrophages and it has been found that many key proteins in immune pathways were modified; however, how itaconate modulates pathogens was not fully understood. Here, we have designed and synthesized a series of itaconate-based bioorthogonal probes, which enable quantitative and site-specific profiling of itaconated proteins and sites in Salmonella . Among many proteins related to energy metabolism, we identified a key enzyme involved in the glyoxylate cycle, isocitrate lyase (ICL), as the most prominent target. Covalent modification of the active-site cysteine in ICL by itaconate abolishes the enzyme activity and suppresses bacterial growth. Our chemoproteomic study has uncovered the wide array of itaconation targets in Salmonella and provided a comprehensive resource for understanding the anti-bacterial function of this intriguing metabolite., Competing Interests: The authors declare no competing interests., (This journal is © The Royal Society of Chemistry.)

RNA-protein interactions play important roles in almost every step of the lifetime of RNAs, such as RNA splicing, transporting, localization, translation and degradation. Post-translational modifications, such as O-GlcNAcylation and phosphorylation, and their "cross-talk" (OPCT) are essential to the activity and function regulation of RNA-binding proteins (RBPs). However, due to the extremely low abundance of O-GlcNAcylation and the lack of RBP-targeted enrichment strategies, large-scale simultaneous profiling of O-GlcNAcylation and phosphorylation on RBPs is still a challenging task. In the present study, we developed a tandem enrichment strategy combining metabolic labeling-based RNA tagging for selective purification of RBPs and HILIC-based enrichment for simultaneous O-GlcNAcylation and phosphorylation profiling. Benefiting from the sequence-independent RNA tagging by ethynyluridine (EU) labeling, 1115 RBPs binding to different types of RNAs were successfully enriched and identified by quantitative mass spectrometry (MS) analysis. Further HILIC enrichment on the tryptic-digested RBPs and MS analysis led to the first large-scale identification of O-GlcNAcylation and phosphorylation in the RNA-binding proteome, with 461 O-GlcNAc peptides corresponding to 300 RBPs and 671 phosphopeptides corresponding to 389 RBPs. Interestingly, ∼25% RBPs modified by two PTMs were found to be related to multiple metabolism pathways. This strategy has the advantage of high compatibility with MS and provides peptide-level evidence for the identification of O-GlcNAcylated RBPs. We expect it will support simultaneous mapping of O-GlcNAcylation and phosphorylation on RBPs and facilitate further elucidation of the crucial roles of OPCT in the function regulation of RBPs.

Conductive hydrogels have attracted widespread attention in wearable electronic devices and human motion detection. However, designing self-healing hydrogels with high conductivity and excellent mechanical properties remains a challenge. In this work, polyvinyl alcohol/carbon nanotubes/graphene (PVA/CNTs/graphene) with an island-bridge hydrogel structure and self-healing properties was designed by merging PVA/CNTs hydrogel and PVA/graphene hydrogel, in which the PVA/graphene hydrogel acts as an "island" and PVA/CNTs hydrogel acts as a "bridge" to bridge the entire conductive network. Hydrogen-bonding between the borate ion and the -OH group of PVA allows the conductive hydrogel to heal without any external stimulation. The PVA/CNTs/graphene hydrogel can be used for both stretchable strain and pressure sensors. The obtained PVA/CNTs/graphene composite hydrogel exhibits excellent electrical conductivity, extreme high elastic strain (up to 900%) and strong mechanical pressure (up to 10 kPa). The strain sensor based on the PVA/CNTs/graphene hydrogel exhibits excellent tensile strain sensitivity (a gauge factor of 152.6 in the strain region of 316-600%) and wide detection working range (1-600%) with high durability and repeatability. The sensor also remains highly sensitive when being used as a pressure sensor (-0.127 kPa-1 at 0-5 kPa). Additionally, the PVA/CNTs/graphene hydrogel-based sensor can detect human motions after multiple cuts and self-healing with excellent stability and repeatability. The PVA/CNTs/graphene hydrogel provides a new idea in the development of wearable electronics, demonstrating the potential of the next generation of wearable electronics.

Optical probes that specifically and sensitively change the optical properties upon contact with targets have become irreplaceable tools in fundamental biology and medicine. Semiconducting polymer dots (Pdots) have emerged as popular optical nanoplatforms because of their excellent characteristics, such as tunable luminescence, high brightness, superior stability and biocompatibility, for biological applications. In particular, facile surface and intra-particle modifications enable Pdots to detect various biological parameters, such as reactive oxygen species (ROS), typical metal ions, pH values, temperature and a variety of biomolecules. In this review, we provide a brief overview of the preparation and bio-functionalization strategies of Pdots. This review focuses on the applications of Pdots as optical probes in biosensors and describes the challenges in this field.

A signal amplification strategy based on the horseradish peroxidase catalyzed polymerization of dopamine on a polymeric ion-selective membrane surface is proposed for the sensitive chronopotentiometric detection of an aptamer-target binding event.

Epilepsy is the fourth most common neurological disorder, and aberrantly elevated sulfur dioxide derivatives (SO 3 2- /HSO 3 - ) are thought to underlie the hippocampal neuronal apoptosis in epilepsy. We have designed and synthesized a mitochondria-targeted polydopamine nanoprobe for visualizing endogenous SO 3 2- /HSO 3 - by the nucleophilic addition reaction. The nanoprobe was used for imaging SO 2 derivatives both in the mitochondria of cultured cells and zebrafish, and successfully applied in the hippocampus of a rat model of epilepsy. The PDAD nanoprobe could be of great value for the elucidation of mechanisms of abnormal SO 3 2- /HSO 3 - involved in diseases such as epilepsy.

We report here a general strategy to read out potentiometric signals via a photoelectrochemical method. The photocurrent at a working electrode coated with a ZnSe/r-GO composite can be modulated by a polymeric membrane ion-selective electrode that works as a reference electrode.

In order to improve active tumor targeting, tumor cell uptake efficiency and circulation time of doxorubicin (DOX) in vivo, we constructed a cleavable PEGylated hyaluronic acid nano-drug delivery system (HA-mPEG2k-DOX) based on a tumor microenvironment pH-responsive imine bond. In this study, HA-mPEG2k-DOX can self-assemble into stable nanoparticles (HA-mPEG2k-DOX NPs) with a particle size of 50 nm. And the NPs can efficiently target CD44 positive CT26 cells and the pH-responsive cleavable PEG shell can be detached under weakly acidic environments and effectively promote the cellular uptake of HA-DOX NPs. Compared with DOX·HCl, the HA-mPEG2k-DOX NPs can significantly increase the DOX circulation time by 12.5 times, efficiently target the tumor tissues of CT26 tumor-bearing mice and remain for 72 hours. Therefore, the antitumor results in vivo indicated that the HA-mPEG2k-DOX NPs have the best anti-tumor effect while reducing the toxicity of the DOX. Overall, the cleavable PEGylated HA-mPEG2k-DOX NPs responding to pH-sensitive imine bonds, while actively targeting CD44-positive tumor cells, improve the dilemma of cellular uptake and delivery by the PEGylated nano delivery system.

A pore-sealing method for preparation of high-permeance alumina microfiltration (MF) membranes free of any intermediate layers is presented. It involves sequential coating of a polyvinyl butyral (PVB) layer and an alumina membrane precursor on the surface of the macroporous alumina support. An alumina MF membrane with no intermediate layers can be obtained on the support after pyrolysis of the PVB interlayer. The interlayer-free membrane prepared by this method has an average pore diameter of 0.26 μm and a water permeance of 1468 ± 81 L m -2 h -1 bar -1 which is prominently higher than that of the ceramic membranes prepared with other techniques. The conspicuous increase of water permeance is speculated mainly due to the filtration resistance decrease of the interlayer-free ceramic membrane., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

An HCBP1 peptide-ruthenium conjugate (Ru-β-Ala-FQHPSFI) as a potential candidate for targeted therapy of hepatoma was synthesized. Ru-β-Ala-FQHPSFI shows drastically enhanced cytotoxicity and high selectivity for hepatoma cells versus noncancer liver cells. Raman imaging shows that this peptide-based drug can be taken up well by the hepatoma cells compared with the bare ruthenium complex (Ru) and the opposite sequence peptide-ruthenium conjugate (Ru-β-Ala-IFSPHQF). This study presents a new strategy for the construction of tumor-targeting metal-based anticancer therapeutics.

Herein, as a proof of concept, we developed the first enzymatic VIE fluorogenic probe for protein tyrosine phosphatase 1B (PTP1B). The detection and imaging of PTP1B using VIE in living cells were both realized. Particularly importantly, the designed probe herein provides a guideline and platform for the development of new VIE-based enzymatic probes.

Two phase photocatalysts can be intergrown with each other, resulting in superior photocatalytic properties. Herein, methylamine lead bromide (MAPbBr 3 ) wrapped/entrapped protonated graphitic carbon nitride (p-g-C 3 N 4 ) intergrowth microcrystals were fabricated by mixing a pervoskite precursor with p-g-C 3 N 4 colloidal sol. A highly stable isopropanol (IPA) solvent based photocatalytic system for dye degradation was demonstrated. The composite with an optimal p-g-C 3 N 4 mass percentage of 3.3 wt% (denoted as MAPbBr 3 /p-g-C 3 N 4 -1.0 mg) exhibited the highest photocatalytic degradation of malachite green (99.8%) within 10 min under visible light, which was 5.3-fold and 16-fold greater than that exhibited by its constituents separately. The strong chemical interaction and fundamental photophysical processes in MAPbBr 3 /p-g-C 3 N 4 were systematically evaluated by spectroscopic and electrochemical techniques, confirming the effective separation of photogenerated electron-hole pairs and faster interfacial charge transfer behavior. Furthermore, active superoxide radicals (O 2 ˙ - ) played a vital role in the catalytic reaction, because of the significant photoinduced electron transfer rate ( k et ) in the inverted type-I core/shell MAPbBr 3 /p-g-C 3 N 4 band configuration structure. In addition, MAPbBr 3 /p-g-C 3 N 4 has good cycling stability for 10 cycles and versatility for other cationic (RhB) and anionic (MO) dye pollutants, indicating the great potential for solar energy conversion into chemical energy., Competing Interests: There are no conflicts to declare., (This journal is © The Royal Society of Chemistry.)

Polysaccharides are one of the major bioactive components in chrysanthemum teas. In order to understand well the chemical structures and bioactivities of polysaccharides from different chrysanthemum teas (JHPs) collected in China, the physicochemical characteristics, antioxidant activity, and antiglycation activity of polysaccharides extracted from different chrysanthemum teas, including Coreopsis tinctoria , Chrysanthemum indicum , C. morifolium 'Huangju', C. morifolium 'Gongju', and C. morifolium 'Hangbaiju', were investigated. The results showed that the contents of total uronic acids and total phenolics in JHPs ranged from (28.4 ± 0.3)% to (36.2 ± 0.2)%, and from 9.4 ± 0.7 to 70.2 ± 1.7 mg GAE per g, respectively. The molecular weights of fraction 1 and fraction 2 in JHPs ranged from 4.29 × 10 5 to 5.88 × 10 5 Da, and from 4.11 × 10 4 to 5.24 × 10 4 Da, respectively. The dominant constituent monosaccharides of JHPs were galacturonic acid, arabinose, and galactose. Furthermore, JHPs, especially polysaccharides extracted from C. tinctoria , exerted remarkable ABTS, DPPH, nitric oxide, and hydroxyl radical scavenging activities, as well as strong antiglycation activities. The results are helpful for better understanding of the chemical structures and bioactivities of JHPs, and JHPs may have good potential applications in the functional-food industry., Competing Interests: The authors declare that there are no conflicts of interest., (This journal is © The Royal Society of Chemistry.)