1. A universal flow cytometry assay for screening carbohydrate-active enzymes using glycan microspheres
- Author
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Taichi E. Takasuka, Brian G. Fox, Lai F. Bergeman, Kai Deng, Anup K. Singh, Paul D. Adams, Chung-Yan Koh, and Aarthi Chandrasekaran
- Subjects
Glycan ,Glycoside Hydrolases ,Biochemical detection ,Catalysis ,Flow cytometry ,Microsphere ,Polysaccharides ,Materials Chemistry ,medicine ,Animals ,Fluorescent Dyes ,chemistry.chemical_classification ,Bioconjugation ,medicine.diagnostic_test ,biology ,Metals and Alloys ,Glycosyltransferases ,General Chemistry ,Flow Cytometry ,Fluorescence ,Microspheres ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,carbohydrates (lipids) ,Enzyme ,chemistry ,Biochemistry ,Ceramics and Composites ,biology.protein ,Carbohydrate active enzymes - Abstract
We describe a simple, multiplexed assay that integrates glycan synthesis, bioconjugation to microspheres, fluorescent chemical/biochemical detection and multiparameter flow cytometric analysis to screen activities of different families of carbohydrate-active enzymes.
- Published
- 2013
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