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Gold nanoparticles (Au NPs) are widely used today in a broad range of applications like electronics, sensors, catalysis and especially in biomedicine. However, the increase in their use has raised concerns about the possible interactions in vivo and the unexpected responses inside humans and other living organisms. Analytical tools that are able to detect their presence in biological samples and also provide information about the presence of dissolved metal ions inside biological samples are, therefore, urgently needed for a proper understanding of the behaviour of these materials. In this work, an HPLC-ICP-MS technique for the determination of Au NPs was adapted to the analysis of tissues from Wistar rats after intraperitoneal injection of 10 nm Au NPs. This technique allows the detection of Au NPs and low-molecular Au species, permitting the detection and monitoring of potential degradation processes. Alkaline and enzymatic digestions were tested to solubilize the particles and to verify their stability during the extraction process. Enzymatic digestion with proteinase K over 14 h was found to be suitable for the extraction of the Au species with recovery rates of about 91%. Finally, the method was applied to the analysis of liver and spleen samples. Significant abundances of low-molecular Au species were detected (around 30%). This observation suggested that a degradation process played a pivotal role during the transport and accumulation of Au NPs that were intraperitoneally injected into Wistar rats.

We have designed and synthesized two novel vanadium coordination compounds using 1H-benzimidazole-2-carboxylic acid and 5-aminopyridine-2-carboxylic acid. These two materials have a mononuclear structure with crystallization water molecules in the network. Both compounds exhibit intense photoluminescence emission at room temperature in the solid state and show in vivo antidiabetic activity together with low in vitro cell toxicities. Luminescence theoretical studies have been performed.