1. A spot test for determination of residual TBA levels in18F-radiotracers for human use using Dragendorff reagent
- Author
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Andrew V. Mossine, Sean S. Tanzey, Alexandra R. Sowa, Jovany Torres, Melanie S. Sanford, Peter Scott, and Allen F. Brooks
- Subjects
Chromatography ,medicine.diagnostic_test ,010405 organic chemistry ,Chemistry ,General Chemical Engineering ,General Engineering ,010402 general chemistry ,Tetrabutylammonium fluoride ,Residual ,01 natural sciences ,High-performance liquid chromatography ,0104 chemical sciences ,Analytical Chemistry ,Human use ,Positron emission tomography ,Reagent ,medicine ,Patient dose - Abstract
When utilizing [18F]tetrabutylammonium fluoride ([18F]TBAF) in the synthesis of 18F-labeled radiotracers for clinical positron emission tomography (PET) imaging, it is necessary to confirm that residual TBA levels in formulated doses do not exceed established specifications (≤2.6 mg per patient dose). Historically this has been accomplished using HPLC, but this is time consuming for short-lived PET radiotracers and limited by the need for expensive equipment. This motivated us to introduce a TLC spot test for determining residual TBA, and we have developed a new method which employs the Dragendorff reagent. Herein we report details of the TLC method and use it to quantify residual TBA in different formulations of 6-[18F]fluoro-DOPA.
- Published
- 2020
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