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Basic and molecular cytogenetic techniques were carried out in 3 Neotropical region populations of catfishes, two of Trachelyopterus galeatus (one from the marshlands of Paraguay River basin and another from Lago Catalão, Amazon River basin) and one of Trachelyopterus porosus, a sympatric population to T. galeatus from the Amazon River basin. This study aimed to describe and understand the structure and evolution of Trachelyopterus B chromosomes, mainly through physical mapping of repetitive elements. A diploid number of 58 chromosomes was found for all individuals, as well as the presence of B chromosomes. For T. porosus this is the first report of a supernumerary. The sympatric species of T. galeatus and T. porosus from Amazon River had 1-3 B chromosomes and T. galeatus from Paraguay River had 1-2 B chromosomes, all of them showed intra- and interindividual numerical variation. Two females of T. porosus exhibited a new variant B chromosome (B2), previously not seen in Auchenipteridae, which might have originated from B1 chromosomes. All B chromosomes were entirely heterochromatic. In contrast to all complement A and B2 chromosomes, in which the telomeric sequences were found in the telomeric regions, B1 chromosomes of all populations were totally marked by (TTAGGG)n probes. (GATA)n sequence sites were found through all complement A chromosomes, but B1 and B2 chromosomes exhibited only a clustered block in one of the chromosome arms. The most frequent B chromosomes (B1) in all populations/species, including those previously studied in Auchenipteridae catfishes, share the following characteristics: totally heterochromatic, small, metacentric, with accumulation of repetitive (TTAGGG)n sequences, and a low number of (GATA)n copies, which might suggest a common ancient origin in Trachelyopterus species/populations., (© 2021 S. Karger AG, Basel.)

The karyotype and chromosomal characteristics of Trachydorasparaguayensis, a representative of the South American catfish family Doradidae, were analyzed by conventional (Giemsa staining, silver staining, C-banding) and molecular (FISH with rDNA and telomeric probes) cytogenetic techniques. The diploid chromosome number was 2n = 56, with 36 metacentric, 16 submetacentric, and 4 subtelocentric chromosomes in both sexes; however, a remarkable heteromorphism in pair 22 (submetacentric and metacentric elements) was detected in 6 individuals. Compared to other representatives of Doradidae which mostly have 58 chromosomes, the karyotype of T. paraguayensis suggests a reduction in 2n due to chromosomal fusion, as could be deduced from the presence of an interstitial telomere sequence in the submetacentric pair 19. Pale heterochromatic blocks were present in the terminal regions of some chromosomes, very similar to other species of Doradidae. The interstitial position of the NORs observed in the karyotype of T. paraguayensis differs from those reported for most Doradidae species, indicating that it is a derived character. FISH with 5S rDNA revealed 2 interstitial fluorescent signals in the submetacentric pair 22, and the polymorphism of these sites likely resulted from a pericentric inversion., (© 2016 S. Karger AG, Basel.)

The karyotype of the Ancistrini catfish Ancistrus taunayi was analyzed by conventional (Giemsa staining, AgNOR staining and C-banding) and molecular cytogenetic (5S and 18S rDNA-FISH) methods. The diploid chromosome number was 2n = 50 (22 metacentrics + 10 submetacentrics + 10 subtelocentrics + 8 acrocentrics) for both sexes. A single NOR-bearing acrocentric chromosome pair (No. 24) was detected after Ag-staining and 18S rDNA-FISH, while 5S rDNA was found only in the subtelocentric pair No. 21. Conspicuous GC-rich heterochromatin blocks corresponded to the NOR sites and were also observed in the distal regions of the acrocentric chromosome pairs Nos. 22 and 25. Chromosome pair No. 22 differed between males and females; in males, only a small interstitial block of GC-rich heterochromatin was present in both chromosomes, whereas in females, 2 blocks of GC-rich heterochromatin flanked a euchromatic region in one of the homologues, suggesting the occurrence of a ZZ/ZW sex chromosome system. Two mechanisms for the origin and evolution of this simple ZZ/ZW sex chromosome system in A. taunayi are proposed: (1) a paracentric inversion followed by amplification of the proximal heterochromatin and (2) amplification of the interstitial heterochromatin followed by a paracentric inversion. Although ZZ/ZW systems have already been described for other Ancistrus species, our results do not show the same pattern, suggesting an independent origin., (© 2015 S. Karger AG, Basel.)

Neotropical fishes have a low rate of chromosome differentiation between sexes. The present study characterizes the first meiotic analysis of sex chromosomes in the order Gymnotiformes. Gymnotus pantanal - females had 40 chromosomes (14m/sm, 26st/a) and males had 39 chromosomes (15m/sm, 24st/a), with a fundamental number of 54 - showed a multiple sexual determination chromosome system of the type X(1)X(1)X(2)X(2)/X(1)X(2)Y. The heterochromatin is restricted to centromeres of all chromosomes of the karyotype. The meiotic behavior of sex chromosomes involved in this system in males is from a trivalent totally pared in the pachytene stage, with a high degree of similarity. The cells of metaphase II exhibit 19 and 20 chromosomes, normal disjunction of sex chromosomes and the formation of balanced gametes with 18 + Y and 18 + X(1)X(2) chromosomes, respectively. The small amount of heterochromatin and repetitive DNA involved in this system and the high degree of chromosome similarity indicated a recent origin of the X(1)X(1)X(2)X(2)/X(1)X(2)Y system in G. pantanal and suggests the existence of a simple ancestral system with morphologically undifferentiated chromosomes., (Copyright © 2011 S. Karger AG, Basel.)

Physical mapping of 5S rDNA in 2 species of knifefishes, Gymnotuspantanal and G. paraguensis (Gymnotiformes), was performed using fluorescence in situ hybridization with a 5S rDNA probe. The 5S rDNA PCR product from the genomes of both species was also sequenced and aligned to determine non-transcribed spacer sequences (NTS). Both species under study had different patterns of 5S rDNA gene cluster distribution. While in the karyotype of G. pantanal two 5S rDNA-bearing pairs were observed, the karyotype of G. paraguensis possessed as many as 19 such pairs. Such multiplication of 5S rDNA gene clusters might be caused by the involvement of transposable elements because the NTS of G. paraguensis was 400 bp long with high identity (90%) with a mobile transposable element called Tc1-like transposon, described from the cyprinid fish Labeo rohita., (Copyright © 2011 S. Karger AG, Basel.)