1. Sonic Hedgehog Signaling Mediates Resveratrol to Increase Proliferation of Neural Stem Cells After Oxygen-Glucose Deprivation/Reoxygenation Injury in Vitro
- Author
-
Li Wang, Changbo Shen, Qin Yang, Fanren Tang, Wei Cheng, Xiaosong Song, Pingping Yu, and Jixiang Chen
- Subjects
Male ,Physiology ,Proliferation ,Hippocampal formation ,Resveratrol ,lcsh:Physiology ,Receptors, G-Protein-Coupled ,Nestin ,Rats, Sprague-Dawley ,chemistry.chemical_compound ,Neural Stem Cells ,Stilbenes ,lcsh:QD415-436 ,Sonic hedgehog ,lcsh:QP1-981 ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,Anti-Inflammatory Agents, Non-Steroidal ,Neurogenesis ,Veratrum Alkaloids ,Smoothened Receptor ,Hedgehog signaling pathway ,Neural stem cell ,Cell biology ,Patched-1 Receptor ,Female ,Signal Transduction ,Patched Receptors ,medicine.medical_specialty ,Cyclopamine ,Cell Survival ,Kruppel-Like Transcription Factors ,Receptors, Cell Surface ,Zinc Finger Protein GLI1 ,lcsh:Biochemistry ,Internal medicine ,medicine ,Animals ,Hedgehog Proteins ,Cell Proliferation ,Sonic hedgehog signaling ,Rats ,Oxygen ,Glucose ,Endocrinology ,nervous system ,chemistry ,biology.protein ,Smoothened ,Reservatrol - Abstract
Background/Aims: There is interest in drugs and rehabilitation methods to enhance neurogenesis and improve neurological function after brain injury or degeneration. Resveratrol may enhance hippocampal neurogenesis and improve hippocampal atrophy in chronic fatigue mice and prenatally stressed rats. However, its effect and mechanism of neurogenesis after stroke is less well understood. Sonic hedgehog (Shh) signaling is crucial for neurogenesis in the embryonic and adult brain, but relatively little is known about the role of Shh signaling in resveratrol-enhanced neurogenesis after stroke. Methods: Neural stem cells (NSCs) before oxygen-glucose deprivation/reoxygenation (OGD/R) in vitro were pretreated with resveratrol with or without cyclopamine. Survival and proliferation of NSCs was assessed by the CCK8 assay and BrdU immunocytochemical staining. The expressions and activity of signaling proteins and mRNAs were detected by immunocytochemistry, Western blotting, and RT-PCR analysis. Results: Resveratrol significantly increased NSCs survival and proliferation in a concentration-dependent manner after OGD/R injury in vitro. At the same time, the expression of Patched-1, Smoothened (Smo), and Gli-1 proteins and mRNAs was upregulated, and Gli-1 entered the nucleus, which was inhibited by cyclopamine, a Smo inhibitor. Conclusion: Shh signaling mediates resveratrol to increase NSCs proliferation after OGD/R injury in vitro.
- Published
- 2015