1. T-Cell-Dependent Popliteal Lymph Node Reactions to Platinum Compounds in Mice
- Author
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Peter Kind, Hans-Christian Schuppe, Ernst Gleichmann, Dagmar Haas-Raida, Johanna Kulig, and Ute Bömer
- Subjects
endocrine system ,Time Factors ,Ratón ,Injections, Subcutaneous ,T-Lymphocytes ,medicine.medical_treatment ,T cell ,Immunology ,Dose-Response Relationship, Immunologic ,Mice, Nude ,Mice, Inbred Strains ,Drug Hypersensitivity ,Leukocyte Count ,Mice ,Subcutaneous injection ,Animals ,Immunology and Allergy ,Medicine ,Lymph node ,Sensitization ,Platinum ,Immunity, Cellular ,business.industry ,Immunogenicity ,Organ Size ,General Medicine ,T lymphocyte ,Disease Models, Animal ,medicine.anatomical_structure ,Lymph Nodes ,business ,Adjuvant - Abstract
The requirements for sensitization to complex salts of platinum were investigated in a mouse model by means of the popliteal lymph node (PLN) assay. A single subcutaneous injection of dissolved hexachloroplatinates without adjuvant induced a vigorous primary immune reaction in the draining PLN. Dose-dependent lymph node activation was determined by an increase in both PLN weight and cellularity. In C57BL/6 mice, peak reactions were obtained around day 6 after administration of 90–180 nmol Na2[PtCl6] or (NH4)2[PtCl6] per animal. Mice primed to [PtCl6]2–– mounted an enhanced response upon local restimulation with suboptimal doses of the same but not unrelated compounds, indicating a specific secondary response. T cells were required to elicit PLN reactions to [PtCl6]2––, because athymic nude mice completely failed to respond, in contrast to their +/nu littermates. Differences between various inbred strains of mice revealed that Pt-induced PLN responses are genetically controlled. Moreover, the immunogenicity of Pt salts in mice is not confined to hexachloroplatinates, but other compounds, such as the antineoplastic agent cis-dichlorodiamine platinum, are able to induce comparable PLN reactions.
- Published
- 1992
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