1. IgA Antibodies Against Gliadin and Tissue Transglutaminase in Dogs With Chronic Enteritis and Intestinal T-Cell Lymphoma.
- Author
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Matsumoto I, Uchida K, Nakashima K, Hiyoshi S, Chambers JK, Tsujimoto H, and Nakayama H
- Subjects
- Animals, Blotting, Western veterinary, Chronic Disease veterinary, Diagnosis, Differential, Dog Diseases diagnosis, Dog Diseases enzymology, Dog Diseases pathology, Dogs, Enteritis enzymology, Enteritis immunology, Enteritis pathology, Enzyme-Linked Immunosorbent Assay veterinary, Female, Immunoglobulin G immunology, Intestinal Neoplasms enzymology, Intestinal Neoplasms immunology, Intestinal Neoplasms pathology, Lymphoma, T-Cell diagnosis, Lymphoma, T-Cell enzymology, Lymphoma, T-Cell immunology, Male, Microscopy, Fluorescence veterinary, Polymerase Chain Reaction veterinary, Protein Glutamine gamma Glutamyltransferase 2, Dog Diseases immunology, Enteritis veterinary, GTP-Binding Proteins immunology, Gliadin immunology, Immunoglobulin A immunology, Intestinal Neoplasms veterinary, Lymphoma, T-Cell veterinary, Transglutaminases immunology
- Abstract
Molecular clonality analysis of T-cell receptor (TCR) genes for diagnosing T-cell lymphoma is widely used in veterinary medicine. However, differentiating chronic enteritis (CE) from intestinal lymphoma is challenging because of the incompatibility between histopathologic and clonality analysis results. On the basis of findings that canine intestinal T-cell lymphoma and celiac disease share some common features, we conducted serologic examinations in combination with histopathologic and T-cell receptor clonality analyses in 48 dogs diagnosed with either CE or intestinal lymphoma. Immunoglobulin A (IgA) and immunoglobulin G (IgG) antibodies against gliadin and tissue transglutaminase (tTG) were quantitatively measured using ELISA. The conditions were classified according to the histopathologic diagnosis, clonality analysis, and combined histopathologic/clonality analysis. Histopathologic analysis showed that dogs with intestinal lymphoma were likely to have high levels of serum IgA antibodies against gliadin and tTG, and serum IgG antibodies against tTG. No correlation between the diagnosed groups and control group was observed in the results of the clonality analysis and histopathologic/clonality analysis. It is interesting that dogs with intestinal lymphoma had a higher serum IgA titer against gliadin and tTG than did dogs with CE. These results suggest an association between repetitive inflammatory stimulation by gliadin peptides and subsequent intestinal lymphoma in dogs.
- Published
- 2018
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