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The focus of this paper is on the reconstruction of the mid-Holocene climatic changes based on the pollen records contained in a 6.2 m thick section in the southern part of the Tengger Desert, northwestern China. To understand the modern analogues of the mid-Holocene changes, modern pollen spectra across different vegetation zones from the Qilian Mountains to the lowland Tengger Desert were studied first. Four pollen combinations were identified, corresponding to four different modern ecological environments: (1) a Picea-Artemisia combination in a spruce forest and its vicinity; (2) an Artemisia-Chenopodiaceae-Gramineae-Salix combination in a mixture belt of montane grassland and poplar woodland; (3) a Chenopodiaceae-Artemisia-Nitraria combination in a desert steppe belt; and (4) an Elaeagnus-Artemisia-Chenopodiaceae-Nitraria combination in a desert riparian forest. Our study also shows that a relatively low percentage of Picea pollen (30–10%) seems to be associated with water transportation (alluvial deposits) and a very low percentage of Picea pollen (10–5%) appears to be related to long-distance air transportation. With references to the modern relationships between pollen assemblages and associated bioclimates, the pollen sequence covering the period between 8500 and 3000 cal. BP was used to infer the climatic change history. The results show that a warmer and drier climate dominated between 8500 and 7950 cal. BP. Subsequent decreases in Artemisia and warm desert forbs/shrubs taxa and contemporaneous fluctuating increases in montane coniferous and aquatics elements from 7950 to 7400 cal. BP reflect a fluctuating increase in humidity and/or a fluctuating decrease in temperature. The following period from 7400 to 5650 cal. BP was generally warm with the Holocene Climate Optimum occurring between 7400 and 6500 cal. BP. A remarkably cool spell followed between 6500 and 6300 cal. BP that was then succeeded by a warmer and more humid period between 6300 and 5650 cal. BP. The period between 5650 and 4450 cal. BP was characterized by drastic oscillations in climatic conditions with three humid and probably cold spells intervened by three relatively dry and warm spells. The period between 4450 and 3500 cal. BP was cold and humid, followed by a wet spell as indicated by a very high percentage of Typha pollen. [ABSTRACT FROM AUTHOR]