1. Cell membrane-mimicking coating for blood-contacting polyurethanes
- Author
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Tomasz Ciach, Maciej Trzaskowski, and Beata A. Butruk-Raszeja
- Subjects
medicine.medical_specialty ,Materials science ,Surface Properties ,Polyurethanes ,Biomedical Engineering ,engineering.material ,Fibrinogen ,Hemolysis ,Dip-coating ,Biomaterials ,chemistry.chemical_compound ,Coated Materials, Biocompatible ,Coating ,Phosphatidylcholine ,Materials Testing ,medicine ,Humans ,Platelet ,Heart prosthesis ,Cell Membrane ,Blood Physiological Phenomena ,Platelet Activation ,medicine.disease ,Surgery ,chemistry ,Wettability ,engineering ,Surface modification ,Adsorption ,Biomedical engineering ,medicine.drug - Abstract
The aim of the present work was to develop simple modification technique for polyurethanes (PUs) intended for use in blood-contacting implants (vascular grafts, heart prosthesis, ventricular assist devices). PU surface was modified with soybean-derived phosphatidylcholine (PC) via one-step dip coating technique. In order to evaluate blood compatibility of the obtained materials, samples were contacted with human blood under static and arterial flow-simulated conditions. The PC-modified surfaces were thoroughly characterized and tested for fibrinogen resistance, the ability to resist platelet adhesion and activation, hemolysis percentage and plasma recalcification time. Results demonstrated significant, more than three-fold reduction in the amount of fibrinogen adsorbed to PC-modified materials as compared to non-modified PU. Analysis of the samples’ surface after incubation with blood showed high reduction in platelet adhesion. The results were confirmed by analysis of blood samples collected after shear-stress tests – the percentage of free (non-aggregated) platelets remaining in blood samples contacted with PC-coated materials exceeded 70%. The same parameter measured for non-modified PU was significantly lower and equaled 28%.
- Published
- 2014
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