1. Effect of berberine on LPS-induced expression of NF-κB/MAPK signalling pathway and related inflammatory cytokines in porcine intestinal epithelial cells
- Author
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Huang Jing, Xu Yuwei, Jin Meilan, Xiong Wen, Wang Zi-li, Li Xueying, Zhang Zhu, Li Chunlin, and Zeng Rongrong
- Subjects
Lipopolysaccharides ,lcsh:Immunologic diseases. Allergy ,0301 basic medicine ,Berberine ,Swine ,Immunology ,Anti-Inflammatory Agents ,Pharmacology ,Microbiology ,NF-κB ,Proinflammatory cytokine ,03 medical and health sciences ,chemistry.chemical_compound ,MAPKs ,0302 clinical medicine ,Phellodendron ,Escherichia coli ,Animals ,Phellodendron chinense ,Intestinal Mucosa ,Medicinal plants ,Molecular Biology ,Cells, Cultured ,Escherichia coli Infections ,Mitogen-Activated Protein Kinase Kinases ,biology ,Alkaloid ,MAPK signalling ,NF-kappa B ,Original Articles ,Cell Biology ,Coptis chinensis ,biology.organism_classification ,030104 developmental biology ,Infectious Diseases ,Gene Expression Regulation ,chemistry ,030220 oncology & carcinogenesis ,porcine intestinal epithelial cells inflammatory factors ,Cytokines ,Inflammation Mediators ,lcsh:RC581-607 ,Coptis ,Signal Transduction - Abstract
Berberine is an alkaloid extracted from medicinal plants such as Coptis chinensis and Phellodendron chinense. It possesses anti-inflammatory, anti-tumour and anti-oxidation properties, and regulates Glc and lipid metabolism. This study explored the mechanisms of the protective effects of berberine on barrier function and inflammatory damage in porcine intestinal epithelial cells (IPEC-J2) induced by LPS. We first evaluated the effects of berberine and LPS on cell viability. IPEC-J2 cells were treated with 5 μg/ml LPS for 1 h to establish an inflammatory model, and 75, 150 and 250 μg/ml berberine were used in further experiments. The expression of IL-1β, IL-6 and TNF-α was measured by RT-PCR. The key proteins of the NF-κB/MAPK signalling pathway (IκBα, p-IκBα, p65, p-p65, c-Jun N-terminal kinase (JNK), p-JNK, p38, p-p38, ERK1/2 and p-ERK1/2) were detected by Western blot. Upon exposure to LPS, IL-1β, IL-6 and TNF-α mRNA levels and p-IκBα p-p65 protein levels were significantly enhanced. Pre-treatment with berberine reduced the expression of inflammatory factors and was positively correlated with its concentration, and dose dependently inhibited the expression of IκBα, p-IκBα, p-p65, p-p38 and JNK. These results demonstrated that pre-treating intestinal epithelial cells with berberine was useful in preventing and treating diarrhoea induced by Escherichia coli in weaned pigs.
- Published
- 2020