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To design titanium (Ti)-based biomaterials with controlled drug-releasing bioactive property, TiO 2 nanotubes with a diameter of approximately 110 nm was fabricated by electrochemical anodization. TiO 2 nanotubes were then loaded with naringin by direct dropping and coated with chitosan layers. The surface morphologies, chemical compositions and wettability of different substrates were characterized by field emission scanning electron microscopy, atomic force microscope, X-ray photoelectron spectroscopy and contact angle measurement, respectively. The in vitro release behavior of naringin was evaluated by UV-visible-spectrophotometer. The biological properties of osteoblasts on different substrates were investigated in vitro. Our results indicate that the chitosan-coated naringin-loaded TiO 2 nanotubes enhanced osteoblast spreading, proliferation, alkaline phosphatase activity and late-stage osteoblast mineralization. This study provides a platform to help enhance osteointegration between the bone and implant surface in clinical applications.

Cardiovascular disease has become a great threat to the health of mankind; current titanium (Ti) stents fail due to late stent thrombosis caused by the lack of re-endothelialization of the Ti stent. The objective of this study was to design a novel cardiovascular Ti implant with improved surface biocompatibility. TiO 2 nanotubes with a diameter of 110 nm were anodized at a constant voltage of 30 V, and fibronectin was immobilized onto the TiO 2 nanotubes using polydopamine. The element composition, morphology, and wettability of the different substrate surfaces were characterized by x-ray photoelectron spectroscopy (XPS), field-emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM), and contact angle measurements, respectively, confirming the successful immobilization of fibronectin. In vitro experiments including immunofluorescence staining, Cell Counting Kit-8 (CCK-8), and nitric oxide (NO) and prostacyclin (PGI2) release demonstrate that fibronectin modified TiO 2 nanotubes supported cell adhesion, proliferation, and normal cellular functions of human umbilical vein endothelial cells (HUVECs). These methodologies can be applied for future fabrication of cardiovascular stents.