1. Methylome and transcriptome signature of bronchoalveolar cells from multiple sclerosis patients in relation to smoking
- Author
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Johan Grunewald, Boel Brynedal, Mikael V. Ringh, Michael Hagemann-Jensen, Jan Wahlström, Susanna Kullberg, Lara Kular, Tomas J. Ekström, Johan Öckinger, Maria Needhamsen, and Maja Jagodic
- Subjects
Multiple Sclerosis ,Bioinformatics ,Transcriptome ,03 medical and health sciences ,Epigenome ,0302 clinical medicine ,Cigarette smoking ,Medicine ,Humans ,Epigenetics ,030304 developmental biology ,0303 health sciences ,Lung ,DNA methylation ,epigenetics ,business.industry ,Multiple sclerosis ,Smoking ,immunopathogenesis ,medicine.disease ,3. Good health ,bronchoalveolar cells ,medicine.anatomical_structure ,Neurology ,Female ,Neurology (clinical) ,sense organs ,business ,Original Research Papers ,030217 neurology & neurosurgery - Abstract
Background: Despite compelling evidence that cigarette smoking impacts the risk of developing multiple sclerosis (MS), little is known about smoking-associated changes in the primary exposed lung cells of patients. Objectives: We aimed to examine molecular changes occurring in bronchoalveolar lavage (BAL) cells from MS patients in relation to smoking and in comparison to healthy controls (HCs). Methods: We profiled DNA methylation in BAL cells from female MS ( n = 17) and HC ( n = 22) individuals, using Illumina Infinium EPIC and performed RNA-sequencing in non-smokers. Results: The most prominent changes were found in relation to smoking, with 1376 CpG sites (adjusted P Conclusions: Our study provides insights into the impact of smoking on lung inflammation and immunopathogenesis of MS.
- Published
- 2020