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16 results on '"Shin, Jae Min"'

15. Asian Sand Dust Upregulates IL-6 and IL-8 via ROS, JNK, ERK, and CREB Signaling in Human Nasal Fibroblasts.

Author

Yang HW, Park JH, Shin JM, Lee HM, and Park IH

Subjects
Cells, Cultured, Cyclic AMP Response Element-Binding Protein metabolism, Extracellular Signal-Regulated MAP Kinases metabolism, Fibroblasts drug effects, Gene Expression Regulation, Humans, Interleukin-6 genetics, Interleukin-8 genetics, MAP Kinase Kinase 4 metabolism, Reactive Oxygen Species metabolism, Signal Transduction, Up-Regulation, Aerosols therapeutic use, Fibroblasts physiology, Interleukin-6 metabolism, Interleukin-8 metabolism, Nose pathology
Published
2020
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16. Effect of doxycycline on epithelial-mesenchymal transition via the p38/Smad pathway in respiratory epithelial cells.

Author

Shin JM, Kang JH, Lee SA, Park IH, and Lee HM

Subjects
A549 Cells, Cadherins metabolism, Cell Movement drug effects, Gene Expression Regulation, Humans, MAP Kinase Signaling System, Primary Cell Culture, Respiratory Mucosa pathology, Smad2 Protein metabolism, Smad3 Protein metabolism, Transforming Growth Factor beta metabolism, Vimentin metabolism, Antineoplastic Agents pharmacology, Doxycycline pharmacology, Epithelial-Mesenchymal Transition drug effects, Respiratory Mucosa drug effects
Abstract

Purpose: Doxycycline has antibacterial and anti-inflammatory effects, and it also suppresses collagen biosynthesis. This study aimed to confirm the effects and mechanism of doxycycline on transforming growth factor (TGF) beta 1 induced epithelial-mesenchymal transition and cell migration in A549 and primary nasal epithelial cells., Methods: A 3-(4,5-dimethylthiazol-2yl)-2,5-diphenyl-tetrazolium bromide assay and phalloidin-fluorescein isothiocyanate staining were used to evaluate cytotoxicity and cellular morphologic changes. Western blot and immunofluorescence staining were used to determine the expression levels of E-cadherin, vimentin, alpha-smooth muscle actin, fibronectin, phosphorylated Smad2/3, and mitogen-activated protein kinases. Scratch and transwell migration assays were used to assess cellular migration ability., Results: Doxycycline (0-10 μg/mL) had no significant cytotoxic effects in A549 and primary nasal epithelial cells. Increased expression of mesenchymal markers, including vimentin, alpha-smooth muscle actin, and fibronectin in TGF beta 1 induced A549 cells were downregulated by doxycycline treatment. In contrast, E-cadherin expression was upregulated in TGF beta 1 induced A549 cells. An in vitro cell migration assay showed that doxycycline also inhibited the ability of TGF beta 1 induced migration. Doxycycline treatment suppressed the activation of Smad2/3 and p38, whereas its inhibitory effects were similar to each element-specific inhibitor in A549 and primary nasal epithelial cells., Conclusion: Doxycycline inhibited TGF beta 1 induced epithelial-to-mesenchymal transition and migration by targeting Smad2/3 and p38 signal pathways in respiratory epithelial cells.

Published
2017
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