1. Controlled Adipose-derived Stromal Cells Differentiation into Adipose and Endothelial Cells in a 3D Structure Established by Cell-assembly Technique.
- Author
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Mingen Xu, Yongnian Yan, Haixia Liu, Rui Yao, and Xiaohong Wang
- Subjects
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ADIPOSE tissues , *TISSUE engineering , *VASCULAR endothelial growth factors , *CELL membrane formation , *CELL differentiation - Abstract
One of the major obstacles in engineering thick and complextissues while vascularizing tissues in vitro is to maintain cell viability duringtissue growth and structural organization. Adipose-derived stromal (ADS) cellswere used to establish a multicellular system through a cell-assembly technique.Attempts were made to control ADS cells differentiation into different targetedcell types according to their positions within an orderly 3D structure. Oil red Ostaining confirmed that the ADS cells in the structure differentiated intoadipocytes with a spherical shape while immunostaining tests confirmed thatthe endothelial growth factor induced ADS cells on the walls of channelsdifferentiated into mature endothelial cells and then organized into tubularstructures throughout the engineered 3D structure. The endothelin-1 and nitricoxide release rules of the endothelial cells were coincidental with those in vivo. This study provides a new approach to engineer orderly endothelialvessel networks in vitro and has potential applications in adipose-tissueengineering. [ABSTRACT FROM AUTHOR]
- Published
- 2009
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