1. Adrenomedullin inhibits the endothelin production induced by urotensin II in rat vascular smooth muscle cells
- Author
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Jun Yang, Yong-Zheng Pang, Chaoshu Tang, Yong-Fen Qi, Yan-Rong Shi, Zhaokang Zhang, and Dingfang Bu
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MAPK/ERK pathway ,medicine.medical_specialty ,Messenger RNA ,Multidisciplinary ,Vascular smooth muscle ,Kinase ,Biology ,Adrenomedullin ,chemistry.chemical_compound ,Endocrinology ,chemistry ,Internal medicine ,cardiovascular system ,medicine ,Extracellular ,Endothelin receptor ,Urotensin-II - Abstract
The aim of this study was to observe the effects of adrenomedullin (ADM) on endothelin (ET) production induced by urotensin II (U II) in rat vascular smooth muscle cells (VSMCs). Cultured VSMCs which were incubated with U II (10−8 mol/L) and with various concentrations of ADM were used to measure the VSMCs 3H-TdR incorporation, the activity of extracellular signal-regulated kinase (ERK), the amount of ET mRNA and ET production in VSMCs. In this work we found that incubation with U II (10−8 mol/L) increased obviously the amount of ET mRNA in VSMCs and ET production in medium, however, co-incubation with ADM (10−10– 10−8 mol/L) and U II(10−8 mol/L) reduced the amount of ET mRNA by 15%, 24% and 45% (P 0.05), 32% (P 0.05), 32% (P < 0.05) and 36% (P < 0.05), respectively, in a concentration-dependent manner. The results show that in cultured VSMCs ADM inhibits ET mRNA expression, ET production and proliferation stimulated by U II, and that inhibitory effect of ADM on U II bioaction could be mediated through inhibiting MAPK pathway.
- Published
- 2002
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