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Furazolidone, a banned nitrofuran antibiotic, is still illegally used in aquaculture and livestock husbandry. A commercial Enzyme-linked Immunosorbent Assay (ELISA) from Europroxima, The Netherlands is described, and validated for the screening of 3-amino-2-oxazolidinone (AOZ), metabolite of furazolidone antibiotic in the muscle of shrimp. Research works was conducted at Veterinary Drug Residue Analysis Laboratory, Institute of Food and Radiation Biology, Atomic Energy Research Establishment, Savar, Dhaka-1349, between July 2018 and December 2019. Validation was done in accordance with the European Commission (EC) decision 2002/657/EC in terms of specificity, detection capability CCβ, selectivity, ruggedness, and stability. Homogenized sample was subjected to acid hydrolysis for releasing of tissue bound metabolite AOZ, and derivatized with o-nitrobenzaldehyde followed by ethyl acetate/n-hexane extraction and detection by ELISA reader. The detection capability CCβ of the method was lower or at 0.3 µg/kg accepting 0% false compliant result. Cut-off factor Fm and the positivity threshold value T was 0.716 and 0.982, respectively. Mean OD of blank sample B was1.225. The assay was valid because T was greater than Fm and smaller than B. The kit was stable even after ten months of storage at recommended temperature and showed good sensitivity. From the practical point of view, the kit studied has good reliability, and could be used in the routine application for the control of illegal use of the drug.

Mustard oil is popular edible oil in Bangladesh. It contains about more than fifty percent erucic acid which is toxic to human beings. This study evaluated the determination of biochemical properties (acid value, iodine value, saponification value and unsaponifiable matter %) of mustard oil, virgin coconut and sesame oil and toxicity determination of these oils through blood indices targeting of these oil by mixing with normal diet as a dietary supplementation. This study found acid value of mustard, sesame and virgin coconut oil was 0.92, 0.312, 0.52 respectively. Iodine value of mustard, sesame and virgin coconut oil were 110.2, 105.2, and 11 respectively. Saponification value of wild mustard, sesame and virgin coconut oil was 171.1, 187.2 and 240 respectively and unsaponifiable matter % of mustard, sesame and virgin coconut oil was 1.19, 0.31 and 0.92 respectively. Also in vivo experiment we divided wistar male rats were 4 groups such as normal diet 12 g/day/rat, mustard oil (MO) 0.6 g/day/rat, virgin coconut oil (VCO) 0.6g/day/rat and sesame oil (SO) 0.6 g/day/rat. In this study the vegetable oil causes the effect on average body weight gain, Food Efficiency Ratio (FER), and lipid profiles [Total cholesterol (Tch), HDL- Cholesterol LDL - Cholesterol, Triglyceride (TG)] and serum enzymes [SGOT (AST), SGPT (ALT)] of different groups of rat also been estimated. The study found that mustard oil significantly (p