Mango (Mangifera indica L.) is an important economical crop in South China. In 2014, a stem-end rot disease was observed on mangoes 10 days after harvest from Sanya city（18.2° N and 106.5° E）, Ledong county（18.5° N and 109.1° E） and Lingshui county（18.5° N and 110.0° E）, Hainan province, China. Initial symptoms were dark brown small spots, quickly darkened and expanded into large spots with irregular margin and internal rotten flesh. Signs of the disease could be observed as fungal mycelium and pycnidia developing on the fruit surface. Twelve diseased fruits collected from 4 places weresurface disinfested using 75% ethanol, segments were dissected from the border between symptomatic and visually healthy peel and placed on potato dextrose agar (PDA) at 25 °C. Four representative isolates ACCC 39317, ACCC 39318, ACCC 39320, and ACCC 39321 were purified using the single spore method and submitted to the Agricultural Culture Collection of China（ACCC）. The initial color of the colonies was translucent white, e...