1. Effects of peptides selected from artificial peptide libraries to p-glycoprotein activity
- Author
-
Sanem Arikan and Aydin Demiray
- Subjects
lcsh:Medicine ,Stimulation ,Peptide ,Recombinant peptides ,law.invention ,multidrug resistance ,law ,Medicine ,Doxorubicin ,Viability assay ,chemistry.chemical_classification ,lcsh:R5-920 ,business.industry ,Drug discovery ,lcsh:R ,Pp-glycoprotein ,Multiple drug resistance ,chemistry ,Biochemistry ,Biyoloji ,Alerji ,Anatomi ve Morfoloji ,Androloji ,Anestezi ,Odyoloji ve Konuşma-Dil Patolojisi ,Biyokimya ve Moleküler Biyoloji ,Biyofizik ,Biyoteknoloji ve Uygulamalı Mikrobiyoloji ,Kalp ve Kalp Damar Sistemi ,Hücre Biyolojisi ,Klinik Nöroloji ,Yoğun Bakım, Tıp ,Dermatoloji ,Acil Tıp ,Endokrinoloji ve Metabolizma ,Gastroenteroloji ve Hepatoloji ,Geriatri ve Gerontoloji ,Sağlık Bilimleri ve Hizmetleri ,Hematoloji ,İmmünoloji ,Enfeksiyon Hastalıkları ,Tamamlayıcı ve Entegre Tıp ,Tıbbi Etik ,Tıbbi İnformatik ,Tıbbi Laboratuar Teknolojisi ,Genel ve Dahili Tıp ,Adli Tıp ,Tıbbi Araştırmalar Deneysel ,Mikrobiyoloji ,Nörolojik Bilimler ,Kadın Hastalıkları ve Doğum ,Onkoloji ,Göz Hastalıkları ,Ortopedi ,Kulak, Burun, Boğaz ,Patoloji ,Pediatri ,Periferik Damar Hastalıkları ,Fizyoloji ,Temel Sağlık Hizmetleri ,Psikiyatri ,Halk ve Çevre Sağlığı ,Radyoloji, Nükleer Tıp, Tıbbi Görüntüleme ,Rehabilitasyon ,Solunum Sistemi ,Romatoloji ,Spor Bilimleri ,Cerrahi ,Transplantasyon ,Tropik Tıp ,Üroloji ve Nefroloji ,Recombinant DNA ,lcsh:Medicine (General) ,business ,membrane biophysics ,medicine.drug ,K562 cells - Abstract
Peptides selected from artificial peptide libraries have special interest in cell targeting, drug discovery, molecular diagnosis and multidrug reversal. Peptides selected from artificial peptide libraries against doxorubicin resistant K562 cells have the capacity in the reversal of the doxorubicin resistance. Our aim is to determine the characteristics of these peptides on recombinant human P-glycoprotein membrane fractions to be able to understand their interactions. Peptides were selected against doxorubicin resistant K562 cells. The effects of synthesized peptides on these cells viability was done by XTT viability assay. The interactions of peptides with P-glycoprotein were done by Pgp-GloTM Assay System by measuring the ATPase activity of P-glycoprotein. According to our results; four selected peptides effected on doxorubicin resistant K562 cells viability and stimulated the P-glycoprotein activity in the presence of doxorubicin at different levels. If the P-glycoprotein stimulation occurs on living doxorubicin resistant K562 cells, cell viability will not be affected due to multidrug resistance effect of P-glycoprotein. Peptides selected from artificial peptide libraries are useful tools in the reversal of multidrug resistance and for understanding the membrane structure-function relationships. [Med-Science 2018; 7(1.000): 158-161]
- Published
- 2018