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Objective To investigate the effects of miR-183-Sp on the proliferation and migration of colorectal cancer (CRC) cells. Methods The miRDB database was used to predict and screen out the upstream target miRNA (miR-183- 5p) of PFN2; the expression levels of miR-183-Sp and PFN2 mRNA in CRC tissues and cell lines were detected by qRTPCR, and we analyzed the correlation between miR-183-Sp and PFN2. Luciferase report gene technology was used to detect the specific binding of miR-183-Sp to PFN2 3' untranslated region (3'UTR); after transfection of CRC cells, the expression of PFN2 was detected by real-time PCR (qRT-PCR) and Western blotting; the proliferation and migration abilities of CRC cells after transfection of miR-183-Sp mimics were detected by CCK-8 and Scratch test. Results Compared with the normal colorectal epithelial cell line and paracancerous tissues, the expression of PFN2 was down-regulated, and miR-183- Sp was up-regulated in CRC cells and cancer tissues (all P < 0. 05); the expression levels of them were negatively correlated (r = -0. 398, P = 0. 042); miR-183-Sp could specifically bind to PFN2 3'UTR. After transfection of miR-183-Sp mimics, the expression of PFN2 (mRN A and protein) in CRC cell lines was inhibited, and the proliferation and migration ab山ties increased (all P <0. 05). Conclusion MiR-183-Sp can promote the proliferation and migration of CRC cells probably by down-regulating PFN2 expression. [ABSTRACT FROM AUTHOR]

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