Search

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

急性肾损伤是多种原因引起的以突发性肾功能不全、含氮产物增加、水电解质和酸碱平衡紊乱等为特点 的一组临床综合征, 呈现高发病率、高病死率的特点。尽管医疗技术不断进步, 但多种方法在治疗急性肾损伤方面 仍有局限性。干细胞移植治疗在急性肾损伤治疗方面有很大前景。近年来, 学者们就胚胎干细胞、胚胎肾或成体肾 中的干/祖细胞、诱导性多能干细胞、人工肾祖细胞、间充质干细胞等多种干细胞治疗急性肾损伤进行了研究, 并取 得一定成果。然而, 多种干细胞的获取和应用仍存在较多难点和问题。肾脏干/祖细胞移植为急性肾损伤治疗提供 了新的方向, 但相关临床应用仍需要深入研究, 如胚胎或成人肾干/祖细胞的特性, 以及多种干细胞的体外扩增方法 和有效移植方法等。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the changes of intestinal flora abundances in the mild cognitive impairment （MCI）patients and the therapeutic effects of Chinese herbal formula Huanshaodan for invigorating spleen and tonifying kidney. Methods A total of 24 patients with MCI were selected and randomly divided into the observation group and control group, with 12 patients in each, and another 12 healthy volunteers of the same period were selected as the healthy control group. The 16S rRNA technique was used to analyze the differences in the abundance of intestinal flora between the MCI patients and healthy controls. In the observation group, Huanshaodan and Eight-section Brocade treatments for invigorating spleen and notifying kidney were applied, while only Eight-section Brocade treatment was applied in the control group, and the duration of treatment in both groups was 12 weeks. The Montreal Cognitive Assessment Scale（MOCA）, Mini Mental State Examination（MMSE）, and TCM syndrome scores were used to evaluate the efficacy and to observe the safety of treatment. Fresh feces were collected from patients with MCI and the abundances of gut microbiota were observed before and after treatment in both groups by sequencing, and the correlations between MOCA and MMSE scores and the abundances of gut microbiota were analyzed. Results Compared with the healthy controls, patients with MCI showed the enrichment of Eisenbergiella, Pasteurellaceae under the phylum of Proteobacteria, and the genus of Haemophilus, and a decrease in Clostridium_XlVa and Oribacterium. The MMSE and MOCA scores of the observation group after treatment were higher than those of the control group, the TCM syndrome score was lower than that of the control group, and the total effective rate of treatment was higher than that of the control group（all P<0. 05）. There were no adverse events during the treatment of MCI patients, and none of the indexes including blood routine, liver and kidney function, and electrocardiogram showed significant abnormalities. At the phylum level, the abundances of Firmicutes, Actinobacteria, Verrucomicrobia, and Proteobacteria decreased and those of Bacteroidetes increased after treatment in MCI patients of both groups（all P<0. 05）. At the genus and species level, the abundances of Clostridium_XlVa and Oribacterium increased and the abundance of Haemophilus decreased in the observation group after treatment（all P<0. 05）. There was no significant difference in the control group before and after treatment. The abundance of Bacteroidetes was positively correlated with MMSE score （r=0. 398, P<0. 05）, the abundance of Clostridium was positively correlated with MoCA and MMSE scores（r=0. 388 and 0. 422, respectively；both P<0. 05）, and the abundances of Haemophilus and Eisenbergiella were negatively correlated with MoCA score (r=0. 372 and - 0. 373, respectively；both P<0. 05）. Conclusions There are significant differences in intestinal flora abundance between MCI patients and healthy people, and the different flora is related to cognitive function. Chinese herbal formula Huanshaodan for invigorating spleen and tonifying kidney is safe and effective in improving cognitive function and decreasing the clinical syndrome of MCI, and its mechanism may be related to the regulation of the gut microbiota in patients. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective To observe the inhibitory effect of isoflavaspidic acid PB on biofilm of Trichophyton rubrum (T. rubrum) and its possible mechanism. Methods After the mature biofilm of T. rubrum was cultured, the minimal inhibitor)' concentrations of isoflavaspidic acid PB, amphotericin B (AMB) and terbinafine hydrochloride (TBF) against the biofilm were determined by the XTT method, then the concentration of drugs inhibiting 50% and 80% biofilm growth (sMIC50 and sMIC80) were calculated. The biofilm of T. rubrum were divided into groups of normal, isoflavaspidic acid PB, AMB and TBF, and the latter 3 groups were treated with isoflavaspidic acid PB. AMB and TBF in the concentration of 125. 00, 2.00, 125.00 μg/mL, respectively. The morphologic change of biofilm was detected by scanning electron microscope (SEM) after treatment of these drugs. The change in content of ergosterol in eytomembrane of biofilm of T. rubrum was determined by ultra-performance liquid chromatography (UPLC). The expression of ERG1 and MEP4 mRNA in biofilm was determined by real-time fluorescence quantitative PCR (qRT-PCR). Results The sMIC50 and sMIC80 of isoflavas-pidie acid PB against biofilm of T. rubrum were 31.25 and 62. 5-125 μg/mL, respectively. The SEM results indicated that, in the isoflavaspidic acid PB group, the growth of biofilm of T. rubrum was significantly restrained, the mycelium was flat and largely broken, and there was no eonidiophores, and a small amount of withered extracellular matrix. The content of ergosterol in the normal group and the group of isoflavaspidic acid PB was (3. 68 ±0. 05) and (2.36 ±0.01) μg/g, the expression of ERG1 mRNA was 1.36 ±0.03 and 1.00 ± 0. 06 , and the expression of MEP4 mRNA WAS 2. 86 ±0. 08 and 1.00 ±0.08. respectively, with significant difference (all P<0.01). Conclusion Isoflavaspidic acid PB has better inhibitory activity against biofilm of T. rubrum by reducing the biosynthesis of ergosterol and inhibiting the ergosterol synthesis-related gene ERG1 and toxic gene MEP4. [ABSTRACT FROM AUTHOR]

Objective To construct biofilm models of Trichophyton rubrum (T. rubrum) and Microsporum canis ( M. canis) and to observe their sensibility to terbinafine (TERB). Methods Standard isolates of T. rubrum and M. canis were inoculated in 96-well plates, at 35 ^ for 6 h or 3 h，respectively，and then we constructed the biofilm models of T. rubrum and M. canis in vitro. The T. rubrum and M. canis biofim morphology at the incubation period of 24，48，72，96 h was observed by inverted microscope. The growing states of T. rubrum and M. canis biofilms were determined by using the XTT assay. Extracellular matrix (ECM) in the mature biofilms of T. rubrum and M. canis were calculated by safranin staining (expressed by OD492). The characteristic of T. rubrum mature biofilm was analyzed by the scanning electron microscope (SEM) . Measuring and comparing the minimal inhibitory concentration ( MIC) as well as the SMIC50，SMICg〇(50% and 80% inhibitory concentration for biofilm) of T. rubrum and M. canis to TERB. Results Yeast cells and its extended product-hypha started to interweave intensively at the time of 48 h. Moreover，two isolates formed lots of ECM which gathered with the hypha at 72 h， and multilayer membranous structure was observed at this time. The mature time of T. rubrum and M. canis biofilms was 96 h and 72 h，respectively，and the OD492 of ECM for two isolates were 1.925 ± 0. 111 and 1. 173 ±0.157，respectively. The hypha of T. rubrum mature biofilm connected with each other, forming the intensive biofilm net. ECM appeared clearly at the periphery of hypha. The MIC of TERB against the planktonic cells of T. rubrum and M. canis were 0. 125 and 1 jxg/mL, respectively, whereas the SMIC50 and SMICg〇 of TERB against two strains biofilm were >256 pg/mL. Conclusion The biofilm models of T. rubrum and M. canis in vitro are constructed successfully. The mature biofilms of T. rubrum and M. canis have low sensitivity to TERB. [ABSTRACT FROM AUTHOR]

Objective To investigate the effect of Rab5 on large-conductance Ca2+ -activated K+ channels (BKca channels) expressed in HEK293 cells. Methods HEK293 cells in the logarithmic phase were randomly divided to four groups: the control group, Rab5WT group, Rab5CA group and Rab5DN group. HEK293 cells were transfected with Flag-hSlo-GFP plasmid in each group and with pcDNA3.1 for control group, Rab5WT for Rab5WT group, Rab5CA for Rab5CA group and Rab5DN for Rab5DN group, respectively. Total 4 μg plasmid with 1: 1 to each plasmid was transfected into 3.5 mm dish. Transfection rate was observed under fluorescence microscopy and the effect of Rab5 on BKca channels was detected by patch clamp technique, Western blotting and flow cytometry when the trasfection rate was above 70%. Results Compared with the control group, Rab5 WT and Rab5 CA significantly increased BKca macroscopic currents, while BKca currents were decreased in the Rab5DN group. Rab5 WT and Rab5 CA increased the expression of membranous BKca, protein in HEK293 cells, while Rab5DN decreased the expression ( all P < 0. 05). Rab5 WT and Rab5 CA increased the Flag+GFP+ ratio (all P<0.05), while Rab5DN decreased the ratio (P<0.05). Conclusion Rab5 significantly in- creases BKca (currents and channel expression on cell surface and may promote the transport process of BKca currents to cell membrane in HEK293 cells. [ABSTRACT FROM AUTHOR]

目的探讨普通性支原体肺炎进展为难治性支原体肺炎的危险因素。方法研究对象为6 401例支原体肺炎患者,经大环内酯类抗菌药物治疗1周及以上,治愈4 753例(普通组),进展为难治性支原体肺炎1 648例(难治组)。比较两组一般资料、临床资料、实验室和影像学检查资料、治疗相关资料,将两组上述有统计学差异指标进行多因素Logistic回归分析。结果两组年龄≥3岁、特应性体质、全身皮疹、发热时间≥10天和住院时间≥14天、大环内酯类抗菌药物应用延迟、发病10天内使用激素、肺部实变影≥2/3肺叶、肺不张或胸膜炎比例以及血清CRP、LDH、PCT水平比较P均<0.05。多因素Logistic回归分析显示,年龄≥3岁、发热时间≥10天、血清CRP水平升高、肺部实变影≥2/3肺叶、肺不张或胸膜炎和大环内酯类抗菌药物应用延迟的95%CI分别为1.001~1.305、1.082~1.459、1.008~1.030、1.067~1.768、1.171~2.562、1.024~1.295,P<0.05或<0.01;特应性体质、全身皮疹、住院时间≥14天、发病10天内使用激素以及血清LDH、PCT水平升高的95%CI分别为0.997~1.289、0.983~1.732、0.998~1.255、1.027~1.304、0.999~1.010、0.819~1.395,P均>0.05。结论年龄≥3岁、发热时间≥10天、血清CRP水平升高、肺部实变影≥2/3肺叶、肺不张或胸膜炎、大环内酯类抗菌药物应用延迟是普通性支原体肺炎进展为难治性支原体肺炎的危险因素。 [ABSTRACT FROM AUTHOR]

Objective To evaluate the diagnostic efficiency of SPECT/CT in diagnosing indeterminate solitary spinal metastasis and assessing the effective radiation dosage. Methods A total of 67 patients with history of primary cancer who had solitary spinal lesions that could not be definitively diagnosed using 99mTc-MDP bone whole body scan were enrolled and SPECT/CT was performed.The pathological results or follow-up (≥6 months) results of X-ray, CT, MRI or SPECT/CT were the standards of final diagnosis .The sensitivity, specificity, accuracy, positive predictive value and negative predictive value of SPECT, CT and SPECT/CT imaging were calculated respectively, and the diagnostic efficiency of SPECT/CT imaging was assessed by using receiver operating characteristic (ROC) analysis in diagnosing indeterminate solitary spinal metastasis.The injected radionuclide activity in patients was calculated for the SPECT /CT inspection, and the dose-length product (DLP) of spiral CT examination in the same machine was also assessed .During the SPECT/CT examination, the effective radiation dosage of radionuclide received in patients with the same machine CT was estimated ( hereinafter referred as the effective dose). Results The sensitivity, specificity, accuracy, negative predictive value and positive predictive value for SPECT/CT imaging were 92.7%, 88.5%, 92.7%, 88.5% and 91.0% respectively, higher than that of SPECT and CT alone. The area under the ROC curve showed that SPECT/CT imaging had the highest diagnostic efficiency in diagnosing indeterminate solitary spinal metastasis (AUC=0.950, P<0.01), and the following was CT and SPECT. The effective doses were 4.01 ±0.35 mSv and 2.49 ±0.97 mSv when patients underwent SPECT/CT examination. Conclusions SPECT/CT imaging was more efficiency as compared with separate sets of SPECT and CT images .The effective radiation dose due to CT increases. [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

难治性腹水是终末期肝硬化的临床表现,治疗难度较大。非选择性β受体阻滞剂(NSBB)因其有明确的降低门静脉压力梯度作用,广泛应用于肝硬化的治疗,疗效确切。对于合并难治性腹水的肝硬化患者是否应用NSBB,学界尚未达成共识。部分学者认为NSBB可加重此类患者的血流动力学紊乱,增加病死率。也有学者发现NSBB治疗晚期肝硬化伴难治性腹水安全且有效,在更多可靠证据出现之前,尚不能将肝硬化伴难治性腹水作为NSBB的禁忌证。NSBB的具体治疗指征、相关禁忌证、治疗时间窗、具体应用剂量等问题仍需进一步探索。 [ABSTRACT FROM AUTHOR]

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Shandong Medical Journal is the property of Shandong Medical Health Newspapers and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

目的：观察加味黄芪桂枝五物汤治疗老年2型糖尿病周围神经病变（DPN ）的效果，探讨其作用机制。方法将90例老年DPN患者随机分为治疗组和对照组各45例，对照组给予糖尿病基础治疗和甲钴胺片口服，治疗组在对照组基础上服用加味黄芪桂枝五物汤，连续治疗8周。比较两组治疗前后的中医证候评分，多伦多临床评分系统（TCSS）评分，血清同型半胱氨酸（Hcy）水平，患侧肢体正中神经和腓总神经的运动神经传导速度（MCV）、感觉神经传导速度（SCV）。结果治疗8周后，治疗组和对照组的中医证候总有效率分别为93．33％和73．33％，两组比较差异有统计学意义（P＜0．05）；治疗组TCSS评分及血清Hcy水平均下降（P均＜0．05），正中神经、腓总神经MCV及SCV均提高（P均＜0．05）。治疗过程中未见明显不良反应。结论加味黄芪桂枝五物汤能改善DPN患者的临床症状，提高神经传导速度，降低血清Hcy水平，治疗DPN安全、有效。 [ABSTRACT FROM AUTHOR]