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Objective To observe the improvement effect of intragastric administration of salidroside (SAL) suspension on acute radiation-induced lung injury (RILI) in mice and to investigate its possible mechanism. Methods Fortyeight C57BL/6J male mice were randomly divided into the low-dose group, medium-dose group, high-dose group, dexamethasone (DXM) group, model group, and control group, with 8 mice in each group. Except for the control group, mice in the other five groups were irradiated with 6 MV-X ray from a medical linear accelerator. On the second day after irradiation, the mice in the low-dose group, medium-dose group and high-dose group were given 15, 30 and 60 mg/kg SAL suspension (once a day, for 7 days), and the mice in the DXM group were given 10 mg/kg DXM (once a day, for 7 days), mice in the normal control group and the model group were both given the same volume of normal saline (once a day, for 7 days). On the second day after the end of intragastric administration, peripheral venous blood of mice in each group was taken, and the lung tissues were taken after the mice were killed. The morphological changes of the lung tissues in each group were observed after HE staining. Serum inflammatory factors [interleukin -1β (IL-1β), interleukin -6 (IL-6) and tumor necrosis factor-α (TNF-α)] were detected by ELISA. The mRNA levels of IL-1β, IL-6 and TNF-α in the lung tissues of mice were detected by real-time fluorescence quantitative PCR. Western blotting was used to detect nuclear factorerythroid 2-related factor 2 (Nrf2), heme oxygenase-1 (HO-1), glutathione peroxidase 4 (GPX4) and transforming growth factor β (TGF-β) in the lung tissues of mice. Results The lung tissue injury of mice in low-, medium- and high-dose groups was improved to different degrees with the most significant improvement in the high-dose group. Compared with the high-dose group, the improvement of lung tissue injury in the mice of the DXM group was more obvious. Compared with the control group, the pulmonary alveolar septa in the model group were diffusely thickened and edematous, the alveolar cavity was obviously reduced or even disappeared, and the normal structure was obviously destroyed. Compared with the control group, the serum levels of IL-1β, IL-6 and TNF-α increased in the model group (all P<0. 05). Compared with the model group, the serum levels of IL-1β, IL-6 and TNF-α decreased in the medium- and high-dose groups and DXM group (all P<0. 05). Compared with the model group, the relative mRNA expression levels of IL-1β, IL-6 and TNF-α in the lung tissues of mice were lower in the medium- and high-dose groups and DXM groups (all P<0. 05). Compared with the control group, the expression levels of Nrf2, HO-1 and GPX4 in the lung tissues of the model group increased, while the expression of TGF-β decreased (all P<0. 05). Compared with the model group, the expression levels of Nrf2, HO-1 and GPX4 in the lung tissues increased, while the expression of TGF-β decreased in the medium- and high-dose groups and DXM group (all P<0. 05). Conclusions Intragastric administration of SAL suspension can improve acute RILI in mice (60 mg/kg SAL suspension has the best effect). The mechanism of SAL suspension in improving acute RILI in mice may be that SAL promotes the expression of Nrf2 and HO-1 protein in the lung tissue and reduces oxidative stress and inflammatory reaction in mice. [ABSTRACT FROM AUTHOR]

关节假体周围感染是人工关节置换术后最严重的并发症之一,常导致患者关节剧烈疼痛,人工关节失效,最终不得不进行翻修手术。糖尿病、营养状态差、病态肥胖、贫血、吸烟史、类风湿性关节炎、肝肾相关疾病等为人工关节置换术后关节假体周围感染的危险因素,称为机体不可改变因素。术前关节腔穿刺、无症状性菌尿、鼻腔定植菌等三个潜在危险因素可在术前进行处理,故称为机体可改变因素;上述三个潜在危险因素可能与关节假体周围感染有关,需纳入术前考虑范畴。 [ABSTRACT FROM AUTHOR]