1. [Biocompatibility of HA/TCP biphasic ceramics with co-cultured human osteoblasts in vitro].
- Author
-
Lu X, Li S, Zhang J, Zhang Z, Lu B, Bu H, Li Y, and Cheng J
- Subjects
- Cell Division, Cells, Cultured, Humans, Osteoblasts cytology, Biocompatible Materials, Calcium Phosphates, Ceramics, Durapatite, Osteoblasts physiology
- Abstract
The biocompatibility of HA/TCP ceramic was evaluated by investigation of attachment and growth of osteoblasts on biomaterial, as well as monitoring the effects of biomaterial on expression of functional phenotypes of co-cultured osteoblasts in vitro. When co-cultured with HA/TCP ceramics, osteoblasts firstly attached to the surface of HA/TCP disk, then attached to notches and grew into the micropores of biomaterial during further culture period. At last, the ceramics were almost packed with osteoblasts. Additionally, osteoblasts co-cultured with HA/TCP were similar to osteoblasts cultured under normal condition in osteoblastic phenotypes; the secreted lots of collagen type I, possess strong activity of Alkaline Phosphatase and mineralized extracellular matrix. The fact that osteoblasts could grow well on HA/TCP ceramics and the biomaterial did not affect their physiological function suggest that HA/TCP ceramic is biocompatible with human osteoblasts.
- Published
- 2001