1. Diagnosis of human herpesvirus 6B primary infection by polymerase chain reaction in young children with exanthematic disease.
- Author
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Magalhães Ide M, Martins RV, Vianna RO, Oliveira SA, and Cavalcanti SM
- Subjects
- Antibody Affinity, Child, Diagnosis, Differential, Exanthema Subitum virology, Fluorescent Antibody Technique, Indirect, Herpesvirus 6, Human immunology, Humans, Polymerase Chain Reaction methods, Saliva virology, Sensitivity and Specificity, Antibodies, Viral blood, DNA, Viral analysis, Exanthema Subitum diagnosis, Herpesvirus 6, Human genetics
- Abstract
Introduction: Exanthem subitum is a classical rash disease of early childhood caused by human herpesvirus 6B (HHV-6B). However, the rash is frequently misdiagnosed as that of either measles or rubella., Methods: In this study, a nested multiplex polymerase chain reaction (PCR) was used to diagnose HHV-6B primary infection, differentiate it from infections caused by HHV-6A and compare it to antibody avidity tests. The samples were separated into case group and control group according to the results of the indirect immunofluorescence assay (IFA) technique., Results: From the saliva samples analyzed, HHV-6A DNA was detected in 3.2% of the case group and in 2.6% of the control group. Regarding HHV-6B, PCR detected viral DNA in 4.8% of the case group and in 1.3% of the control group. Among the serum samples studied, a frequency of 1.7% was determined for HHV-6A in the case group and 1.2% in the control group. PCR did not detect HHV-6B DNA in serum samples. The sensitivity and specificity of the PCR technique ranged from 0% to 4.8% and 97.5% to 100%, respectively, compared to IFA., Conclusions: The PCR technique was not suitable for diagnosing primary infection by HHV-6B in children with exanthematic disease and should not substitute the IFA.
- Published
- 2011
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