WOS: 000361556500025 Poly(vinylferrocenium) perchlorate-poly(3,3'-diaminobenzidine) (PVF+ClO4--PDAB) composite film was synthesized electrochemically for the first time on Pt disc electrode in a dichloromethane solution containing poly(vinylferrocene) (PVF) polymer, 3,3'-diaminobenzidine (DAB) monomer and tetrabutylammonium perchlorate as supporting electrolyte. This modified electrode was characterized using cyclic voltammetry, Fourier transform infrared spectroscopy (FTIR), UV-Vis spectrophotometry, atomic absorption spectroscopy (AAS), scanning electron microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) methods and it was used for amperometric determination of hydroquinone (HQ) at potential ranges of 0.40 and 0.60 V. the best analytical results for HQ was obtained at 0.45 V in NaHSO4/Na2SO4 solution (pH 2.0) and limit of detection, limit of quantification and linear response range were found as 7.469 x 10(-5), 2.489 x 10(-4) and 2.489 x 10(-4)-65.0 mmol L-1 (R-2 = 0.996), respectively. These results were compared to poly(3,3'-diaminobenzidine) (PDAB) coated and uncoated Pt electrodes. the best analytical results were obtained with composite film because of behavior as electron transfer mediator of PVF. Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [111T445]; Recep Tayyip Erdogan University Scientific Research Projects UnitRecep Tayyip Erdogan University [2013.102.02.3] Support to this work by the Scientific and Technological Research Council of Turkey (TUBITAK) (Project No. 111T445) and Recep Tayyip Erdogan University Scientific Research Projects Unit (Project No. 2013.102.02.3) is greatly appreciated. We thank Associate Professor Dr Selcuk Demir (Recep Tayyip Erdogan University, Faculty of Sciences & Arts, Department of Chemistry, Rize, Turkey) for improving the language of this manuscript.