1. Staphylococcus petrasii subsp. pragensis subsp. nov., occurring in human clinical material
- Author
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Tereza Gelbíčová, Ivana Mašlanˇová, Peter Vandamme, Jitka Černohlávková, Annelies De Bel, Ivana Varbanovová, Ivo Sedláček, Fedoua Echahidi, Margo Cnockaert, Petr Petráš, Roman Pantu˚ček, Pavel Švec, Supporting clinical sciences, Microbiology and Infection Control, and Clinical Biology
- Subjects
DNA, Bacterial ,Sequence analysis ,Staphylococcus ,Molecular Sequence Data ,Biology ,Subspecies ,medicine.disease_cause ,phylogeny ,Microbiology ,Ribotyping ,RNA, Ribosomal, 16S ,medicine ,Journal Article ,Humans ,Ecology, Evolution, Behavior and Systematics ,Czech Republic ,Base Composition ,DNA–DNA hybridization ,Research Support, Non-U.S. Gov't ,Fatty Acids ,Nucleic Acid Hybridization ,General Medicine ,Chaperonin 60 ,Sequence Analysis, DNA ,Ribosomal RNA ,rpoB ,16S ribosomal RNA ,Genes, Bacterial ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Oxidoreductases - Abstract
Seven coagulase-negative, oxidase-negative and novobiocin-susceptible staphylococci assigned tentatively as Staphylococcus petrasii were investigated in this study in order to elucidate their taxonomic position. All strains were initially shown to form a genetically homogeneous group separated from remaining species of the genus Staphylococcus by using a repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Phylogenetic analysis based on 16S rRNA gene, hsp60, rpoB, dnaJ, gap and tuf sequences showed that the group is closely related to Staphylococcus petrasii but separated from the three hitherto known subspecies, S. petrasii subsp. petrasii, S. petrasii subsp. croceilyticus and S. petrasii subsp. jettensis. Further investigation using automated ribotyping, MALDI-TOF mass spectrometry, fatty acid methyl ester analysis, DNA–DNA hybridization and extensive biotyping confirmed that the analysed group represents a novel subspecies within S. petrasii, for which the name Staphylococcus petrasii subsp. pragensis subsp. nov. is proposed. The type strain is NRL/St 12/356T ( = CCM 8529T = LMG 28327T).
- Published
- 2015
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