7 results on '"Furness, David N"'
Search Results
2. Evidence for Opening of Hair-Cell Transducer Channels after Tip-Link Loss
- Author
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Meyer, Jens, primary, Furness, David N., additional, Zenner, Hans-Peter, additional, Hackney, Carole M., additional, and Gummer, Anthony W., additional
- Published
- 1998
- Full Text
- View/download PDF
3. The Mechanosensory Structure of the Hair Cell Requires Clarin-1, a Protein Encoded by Usher Syndrome III Causative Gene.
- Author
-
Ruishuang Geng, Melki, Sami, Chen, Daniel H.-C., Tian, Guilian, Furness, David N., Tomoko Oshima-Takago, Neef, Jakob, Moser, Tobias, Askew, Charles, Horwitz, Geoff, Holt, Jeffrey R., Yoshikazu Imanishi, and Alagramam, Kumar N.
- Subjects
HAIR cells ,USHER'S syndrome ,GENETIC mutation ,TETRASPANIN ,IMMUNOHISTOCHEMISTRY ,SYNAPSES ,LABORATORY mice - Abstract
Mutation in the clarin-1 gene (Clrn1) results in loss of hearing and vision in humans(Ushersyndrome III), but the role of clarin-1 in the sensory hair cells is unknown. Clarin-1 is predicted to be a four transmembrane domain protein similar to members of the tetraspanin family. Mice carrying null mutation in the clarin-1 gene (Clrn1
-/- )show loss of hair cell function and a possible defect in ribbon synapse. We investigated the role of clarin-1 using various in vitro and in vivo approaches. We show by immunohistochemistry and patch-clamp recordings of Ca2+ currents and membrane capacitance from inner hair cells that clarin-1 is not essential for formation or function of ribbon synapse. However, reduced cochlear microphonic potentials, FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] loading, and transduction currents pointed to diminished cochlear hair bundle function in Clrn1-/- mice. Electron microscopy of cochlear hair cells revealed loss of some tall stereocilia and gaps in the v-shaped bundle, although tip links and staircase arrangement of stereocilia were not primarily affected by Clrn1-/- mutation. Human clarin-1 protein expressed in transfected mouse cochlear hair cells localized to the bundle; however, the pathogenic variant p.N48K failed to localize to the bundle. The mouse model generated to study the in vivo consequence of p.N48K in clarin-1 (Clrn1N48K ) supports our in vitro and Clrn1-/- mouse data and the conclusion that CLRN1 is an essential hair bundle protein. Furthermore, the ear phenotype in the Clrn1N48K mouse suggests that it is a valuable model for ear disease in CLRN1N48K , the most prevalent Usher syndrome III mutation in North America. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
4. The Density of EAAC1 (EAAT3) Glutamate Transporters Expressed by Neurons in the Mammalian CNS.
- Author
-
Holmseth, Silvia, Dehnes, Yvette, Huang, Yanhua H., Follin-Arbelet, Virginie V., Grutle, Nina J., Mylonakou, Maria N., Plachez, Celine, Yun Zhou, Furness, David N., Bergles, Dwight E., Lehre, Knut P., and Danbolt, Niels C.
- Subjects
GLUTAMATE transporters ,NEURONS ,AMINO acids ,EXCITATORY amino acids ,GENE expression ,HIPPOCAMPUS (Brain) ,METHYL aspartate receptors - Abstract
The extracellular levels of excitatory amino acids are kept low by the action of the glutamate transporters. Glutamate/aspartate transporter (GLAST) and glutamate transporter-1 (GLT-1) are the most abundant subtypes and are essential for the functioning of the mammalian CNS, but the contribution of the EAAC1 subtype in the clearance of synaptic glutamate has remained controversial, because the density of this transporter in different tissues has not been determined. We used purified EAAC1 protein as a standard during immunoblotting to measure the concentration of EAAC1 in different CNS regions. The highest EAAC1 levels were found in the young adult rat hippocampus. Here, the concentration of EAAC1 was∼0.013 mg/g tissue (∼130 moleculesµm
-3 ), 100 times lower than that of GLT-1. Unlike GLT-1 expression, which increases in parallel with circuit formation, only minor changes in the concentration of EAAC1 were observed from E18 to adulthood. In hippocampal slices, photolysis of MNI-D-aspartate (4-methoxy-7-nitroindolinyl-D-aspartate) failed to elicit EAAC1-mediated transporter currents in CA1 pyramidal neurons, and D-aspartate uptake was not detected electron microscopically in spines. Using EAAC1 knock-out mice as negative controls to establish antibody specificity, we show that these relatively small amounts of EAAC1 protein are widely distributed in somata and dendrites of all hippocampal neurons. These findings raise new questions about how so few transporters can influence the activation of NMDA receptors at excitatory synapses. [ABSTRACT FROM AUTHOR]- Published
- 2012
- Full Text
- View/download PDF
5. Rapid, Learning-Induced Inhibitory Synaptogenesis in Murine Barrel Field.
- Author
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Jasinska, Malgorzata, Siucinska, Ewa, Cybulska-Klosowicz, Anita, Pyza, Elzbieta, Furness, David N., Kossut, Malgorzata, and Glazewski, Stanislaw
- Subjects
SYNAPSES ,NEURAL transmission ,TRANSMISSION electron microscopy ,GABA ,CONDITIONED response - Abstract
The structure of neurons changes during development and in response to injury or alteration in sensory experience. Changes occur in the number, shape, and dimensions of dendritic spines together with their synapses. However, precise data on these changes in response to learning are sparse. Here, we show using quantitative transmission electron microscopy that a simple form of learning involving mystacial vibrissae results in ~70% increase in the density of inhibitory synapses on spines of neurons located in layer IV barrels that represent the stimulated vibrissae. The spines contain one asymmetrical (excitatory) and one symmetrical (inhibitory) synapse (double-synapse spines), and their density increases threefold as a result of learning with no apparent change in the density of asymmetrical synapses. This effect seems to be specific for learning because pseudoconditioning (in which the conditioned and unconditioned stimuli are delivered at random) does not lead to the enhancement of symmetrical synapses but instead results in an upregulation of asymmetrical synapses on spines. Symmetrical synapses of cells located in barrels receiving the conditioned stimulus also show a greater concentration of GABA in their presynaptic terminals. These results indicate that the immediate effect of classical conditioning in the "conditioned" barrels is rapid, pronounced, and inhibitory. [ABSTRACT FROM AUTHOR]
- Published
- 2010
- Full Text
- View/download PDF
6. The Dimensions and Composition of Stereociliary Rootlets in Mammalian Cochlear Hair Cells: Comparison between High- and Low-Frequency Cells and Evidence for a Connection to the Lateral Membrane.
- Author
-
Furness, David N., Mahendrasingam, Shanthini, Ohashi, Mitsuru, Fettiplace, Robert, and Hackney, Carole M.
- Subjects
- *
HAIR cells , *CORTI'S organ , *MECHANORECEPTORS , *TROPOMYOSINS , *NEUROSCIENCES - Abstract
The sensory bundle of vertebrate cochlear hair cells consists of actin-containing stereocilia that are thought to bend at their ankle during mechanical stimulation. Stereocilia have dense rootlets that extend through the ankle region to anchor them into the cuticular plate. Because this region may be important in bundle stiffness and durability during prolonged stimulation at high frequencies, we investigated the structure and dimensions of rootlets relative to the stereocilia in apical (low-frequency) and basal (high-frequency) regions of rodent cochleae using light and electron microscopy. Their composition was investigated using postembedding immunogold labeling of tropomyosin, spectrin, β-actin, γ-actin, espin, and prestin. The rootlets have a thick central core that widens at the ankle, and are embedded in a filamentous meshwork in the cuticular plate. Within a particular frequency region, rootlet length correlates with stereociliary height but between regions it changes disproportionately; apical stereocilia are, thus, approximately twice the height of basal stereocilia in equivalent rows, but rootlet lengths increase much less. Some rootlets contact the tight junctions that underlie the ends of the bundle. Rootlets contain spectrin, tropomyosin, and β- and γ-actin, but espin was not detected; spectrin is also evident near the apical and junctional membranes, whereas prestin is confined to the basolateral membrane below the junctions. These data suggest that rootlets strengthen the ankle region to provide durability and may contact with the lateral wall either to give additional anchoring of the stereocilia or to provide a route for interactions between the bundle and the lateral wall. [ABSTRACT FROM AUTHOR]
- Published
- 2008
- Full Text
- View/download PDF
7. The mechanosensory structure of the hair cell requires clarin-1, a protein encoded by Usher syndrome III causative gene.
- Author
-
Geng R, Melki S, Chen DH, Tian G, Furness DN, Oshima-Takago T, Neef J, Moser T, Askew C, Horwitz G, Holt JR, Imanishi Y, and Alagramam KN
- Subjects
- Acoustic Stimulation, Age Factors, Alcohol Oxidoreductases metabolism, Animals, Animals, Newborn, Asparagine genetics, Barium pharmacology, Biophysical Phenomena genetics, Cadherins genetics, Cell Line, Transformed, DNA-Binding Proteins metabolism, Disease Models, Animal, Evoked Potentials, Auditory, Brain Stem genetics, Green Fluorescent Proteins genetics, Green Fluorescent Proteins metabolism, Hair Cells, Auditory ultrastructure, Humans, Lysine genetics, Membrane Potentials drug effects, Membrane Potentials genetics, Membrane Proteins deficiency, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microscopy, Electron, Scanning methods, Mutation genetics, Nerve Fibers pathology, Nerve Fibers ultrastructure, Organ Culture Techniques, Patch-Clamp Techniques, Physical Stimulation methods, Psychoacoustics, Pyridinium Compounds metabolism, Quaternary Ammonium Compounds metabolism, Receptors, AMPA metabolism, Synapses pathology, Synapses ultrastructure, Transfection, Usher Syndromes pathology, Usher Syndromes physiopathology, Cochlea cytology, Cochlea growth & development, Hair Cells, Auditory physiology, Mechanoreceptors physiology, Membrane Proteins genetics, Usher Syndromes genetics
- Abstract
Mutation in the clarin-1 gene (Clrn1) results in loss of hearing and vision in humans (Usher syndrome III), but the role of clarin-1 in the sensory hair cells is unknown. Clarin-1 is predicted to be a four transmembrane domain protein similar to members of the tetraspanin family. Mice carrying null mutation in the clarin-1 gene (Clrn1(-/-)) show loss of hair cell function and a possible defect in ribbon synapse. We investigated the role of clarin-1 using various in vitro and in vivo approaches. We show by immunohistochemistry and patch-clamp recordings of Ca(2+) currents and membrane capacitance from inner hair cells that clarin-1 is not essential for formation or function of ribbon synapse. However, reduced cochlear microphonic potentials, FM1-43 [N-(3-triethylammoniumpropyl)-4-(4-(dibutylamino)styryl) pyridinium dibromide] loading, and transduction currents pointed to diminished cochlear hair bundle function in Clrn1(-/-) mice. Electron microscopy of cochlear hair cells revealed loss of some tall stereocilia and gaps in the v-shaped bundle, although tip links and staircase arrangement of stereocilia were not primarily affected by Clrn1(-/-) mutation. Human clarin-1 protein expressed in transfected mouse cochlear hair cells localized to the bundle; however, the pathogenic variant p.N48K failed to localize to the bundle. The mouse model generated to study the in vivo consequence of p.N48K in clarin-1 (Clrn1(N48K)) supports our in vitro and Clrn1(-/-) mouse data and the conclusion that CLRN1 is an essential hair bundle protein. Furthermore, the ear phenotype in the Clrn1(N48K) mouse suggests that it is a valuable model for ear disease in CLRN1(N48K), the most prevalent Usher syndrome III mutation in North America.
- Published
- 2012
- Full Text
- View/download PDF
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