1. Long non‑coding RNA CCAT2 promotes epithelial‑mesenchymal transition involving Wnt/β‑catenin pathway in epithelial ovarian carcinoma cells.
- Author
-
Wang, Beidi, Liu, Meimei, Zhuang, Rujin, Jiang, Jing, Gao, Jiayin, Wang, Hao, ChEN, He, Zhang, ZongfENg, Kuang, Ye, and Li, Peiling
- Subjects
CANCER genetics ,OVARIAN cancer ,NON-coding RNA ,OVARIAN cancer treatment ,EPITHELIAL cells ,CATENINS ,WNT signal transduction ,CANCER cells - Abstract
Long non‑coding RNA colon cancer‑associated transcript 2 (CCAT2) is dysregulated in a variety of types of human cancer. However, the role of CCAT2 in epithelial ovarian carcinoma (EOC) remains largely unknown. The aim of this study is to investigate the effect of CCAT2 on epithelial‑mesenchymal transition (EMT) and related molecular mechanisms in epithelial ovarian cancer cells. In the current paper, we found that CCAT2 was significantly upregulated in EOC SKOV3, A2780 and HO8910 cell lines compared with the normal ovarian epithelial HUM‑CELL‑0088 cell line. Functional assays demonstrated that the knockdown of CCAT2 inhibited migration and invasion of EOC cells in vitro. Moreover, our results showed that silencing CCAT2 inhibited EMT by the upregulation of epithelial cadherin and downregulation of neural cadherin, zinc finger protein SNAI and Twist‑related protein 1 in SKOV3 and A2780 cell lines. But, that was reversed by the treatment with lithium chloride (LiCl), by which the canonical Wnt/β‑catenin pathway could be activated. In addition, we further investigated the role of CCAT2 in the modulation of Wnt/β‑catenin signaling pathway. Our results revealed that knockdown of CCAT2 inhibited the expression of β‑catenin and the activity of T‑cell factor/lymphoid enhancer factor, acting as a key transcription factor of Wnt signaling pathway. Collectively, these results indicate that CCAT2 may promote EMT, at least partly through Wnt/β‑catenin signaling pathway in EOC cells. Thus, CCAT2 might play a critical role in EOC progression and serve as a valuable target for the treatment of ovarian cancer. [ABSTRACT FROM AUTHOR]
- Published
- 2018
- Full Text
- View/download PDF