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Lung cancer is a major cause of death worldwide, and non‑small cell lung cancer (NSCLC) is the most common type of lung cancer. The aim of this study was to investigate whether miR‑96 mediated the invasion and metastasis of NSCLC by targeting glypican‑3 (GPC3). Reverse transcription‑quantitative PCR (RT‑qPCR) was employed to detect the level of miR‑96 and GPC3 mRNA. We applied western blot analysis to measure the protein expression level of GPC3 gene. The luciferase reporter assay was employed to confirm that GPC3 was a target gene of miR‑96. The Transwell assay was used to detect migration and invasion. The results revealed that miR‑96 was upregulated in NSCLC tissues and lung cancer cells (A549 and H460) compared with corresponding paracancerous tissues and normal epidermic MRC‑5 cells. Overexpression of miR‑96 promoted invasion and migration in A549 cells. GPC3 was a direct target of miR‑96 and regulated by miR‑96. GPC3 could reverse partial fuction of miR‑96 on proliferation. In conclusion, miR‑96 was able to promote the migration and invasion of lung cancer cells by targeting GPC3 gene. The newly identified miR‑96/GPC3 axis may provide a therapeutic method for the treatment of NSCLC. [ABSTRACT FROM AUTHOR]