1. Flow-Injection Lucigenin-Cu(III) Complex Chemiluminescence Determination of Cysteine and Glutathione in Pharmaceutical Formulations
- Author
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Ahmed, Zahoor, Yaqoob, Mohammad, Asghar, Muhammad, Ali, Samar, Munawar, Nusrat, Achakzai, Abdul Kabir Khan, and Nabi, Abdul
- Subjects
Cystine ,Thiols ,Cysteine ,Chemistry - Abstract
A flow injection method for the determination of cysteine (CySH) and glutathione (GSH) amino acids is reported based on lucigenin-diperiodatocuprate(III) complex chemiluminescence (CL) reaction in basic medium. Under optimum conditions, linear calibration curves were obtained in the range of 0.001-30 (R.sup.2 = 0.9998, n = 6) and 0.005-30 mg/L (R.sup.2 = 0.9989, n = 6) with detection limits (S/N = 3) of 2.5 x 10.sup.-4 and 8 x 10.sup.-4 mg/L for CySH and GSH, respectively. The relative standard deviation was 4.1% (n = 11) with injection throughput of 160 per hour. We have applied the developed method to determine CySH and GSH in pharmaceutical samples and compared the results with a spectrophotometric method. Statistical tests such as Student's t- and F-tests were applied for both results with no significant difference observed at a 95% confidence level. The CL reaction of lucigenin-copper(III) complex is also presented., Author(s): Zahoor Ahmed [sup.1], Mohammad Yaqoob [sup.1], Muhammad Asghar [sup.1], Samar Ali [sup.1], Nusrat Munawar [sup.2], Abdul Kabir Khan Achakzai [sup.3], Abdul Nabi [sup.1] Author Affiliations: (1) grid.413062.2, Department of [...]
- Published
- 2021
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