1. Monodispersed molecularly imprinted polymer for creatinine by modified precipitation polymerization.
- Author
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Haginaka J, Miura C, Funaya N, and Matsunaga H
- Subjects
- Acetates chemistry, Acetonitriles chemistry, Buffers, Chemistry Techniques, Analytical methods, Creatine chemistry, Humans, Hydantoins chemistry, Kinetics, Microscopy, Electron, Scanning methods, Models, Chemical, Pyrrolidinones chemistry, Succinimides chemistry, Time Factors, Creatinine chemistry, Molecular Imprinting methods, Polymers chemistry
- Abstract
A monodispersed molecularly imprinted polymer (MIP) for creatinine was prepared by modified precipitation polymerization. The retention and molecular-recognition properties of the prepared MIP were evaluated by the hydrophilic interaction chromatography mode using a mixture of ammonium acetate buffer and acetonitrile as a mobile phase in liquid chromatography. The MIP had a specific recognition ability for creatinine, while other structurally related compounds, such as hydantoin, 1-methylhydantoin, 2-pyrrolidone, N-hydroxysuccinimide and creatine, could not be recognized on the MIP. In addition to shape recognition, hydrophilic interactions could work for the recognition of creatinine on the MIP.
- Published
- 2012
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