1. Autotaxin (NPP-2) in the brain: cell type-specific expression and regulation during development and after neurotrauma.
- Author
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Savaskan NE, Rocha L, Kotter MR, Baer A, Lubec G, van Meeteren LA, Kishi Y, Aoki J, Moolenaar WH, Nitsch R, and Bräuer AU
- Subjects
- Animals, COS Cells, Chlorocebus aethiops, DNA Primers, Female, Fluorescent Antibody Technique, Immunohistochemistry, In Situ Hybridization, Lysophospholipids biosynthesis, Male, Neuroglia metabolism, Rats, Rats, Sprague-Dawley, Reverse Transcriptase Polymerase Chain Reaction, Signal Transduction physiology, Brain metabolism, Brain Injuries metabolism, Gene Expression Regulation, Developmental, Gene Expression Regulation, Enzymologic, Phosphoric Diester Hydrolases metabolism, Pyrophosphatases metabolism
- Abstract
Autotaxin is a secreted cell motility-stimulating exo-phosphodiesterase with lysophospholipase D activity that generates bioactive lysophosphatidic acid. Lysophosphatidic acid has been implicated in various neural cell functions such as neurite remodeling, demyelination, survival and inhibition of axon growth. Here, we report on the in vivo expression of autotaxin in the brain during development and following neurotrauma. We found that autotaxin is expressed in the proliferating subventricular and choroid plexus epithelium during embryonic development. After birth, autotaxin is mainly found in white matter areas in the central nervous system. In the adult brain, autotaxin is solely expressed in leptomeningeal cells and oligodendrocyte precursor cells. Following neurotrauma, autotaxin is strongly up-regulated in reactive astrocytes adjacent to the lesion. The present study revealed the cellular distribution of autotaxin in the developing and lesioned brain and implies a function of autotaxin in oligodendrocyte precursor cells and brain injuries.
- Published
- 2007
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