1. Second harmonic generation imaging of the pig lamina cribrosa using a scanning laser ophthalmoscope-based microscope.
- Author
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Agopov M, Lomb L, La Schiazza O, and Bille JF
- Subjects
- Animals, Image Processing, Computer-Assisted, Intraocular Pressure, Microscopy, Confocal instrumentation, Microscopy, Confocal methods, Models, Animal, Ophthalmoscopes, Optic Disk physiology, Ophthalmoscopy methods, Optic Disk anatomy & histology, Sus scrofa anatomy & histology
- Abstract
We describe a novel scanning laser ophthalmoscope (SLO)-based on a video-rate second harmonic generation imaging microscope. A titanium:sapphire femtosecond laser was coupled to a modified SLO. The laser beam was scanned over the sample, and the light produced by second harmonic generation (SHG) was collected for imaging at video-speed. The device was used for imaging the lamina cribrosa (LC) of enucleated pig eyes. A resolution comparable to that of commercial multiphoton microscopes was reached. The SHG images were used for determining the average pore size of the LC determined from the images; the pressure dependence of the pore size was studied by the artificial increasing of the hydrostatic pressure in the eye. A pressure increase of 44.3 mmHg enlarged the average pore size of 62 analyzed pores by a statistically significant amount. The relative pore growth was measured at four different pressure levels in 25 pores. The pressure was increased in 15 mmHg steps. A general tendency for monothonic growth was observed, although single pores grew by no means linearly.
- Published
- 2009
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