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Various energy exist everywhere around us, it is expected to simultaneously harvest them from multiple sources to power micro/nano electronic system and even personal electronic products. In this work, we proposed a hybrid energy harvesting system (HEHS) for potential in vivo applications. The HEHS consisted of a triboelectric nanogenerator (TENG) and a glucose fuel cell (GFC) for simultaneously harvesting biomechanical energy and biochemical energy in simulated body fluid. These two energy-harvesting units can work individually as a single power source or work simultaneously as an integrated system. This design strengthened the flexibility of harvesting multiple energy and enhanced corresponding electric output. Compared with any individual device, the integrated HEHS outputs a superimposed current and has a faster charging rate. Using the harvested energy, HEHS can power a calculator or a green light emitting diode (LED) pattern. Considering the widely-existed biomechanical energy and glucose molecules in body, the developed HEHS can be a promising candidate for building in-vivo self-powered healthcare monitoring system.