1. The role of toll-like receptors (TLR-2 and -4) and triggering receptor expressed on myeloid cells 1 (TREM-1) in human term and preterm labor.
- Author
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Youssef RE, Ledingham MA, Bollapragada SS, O'Gorman N, Jordan F, Young A, and Norman JE
- Subjects
- Adult, Biopsy, Contraceptive Agents, Female pharmacology, Drug Interactions, Female, Gene Expression immunology, Humans, Immunohistochemistry, Lipopolysaccharides pharmacology, Medroxyprogesterone Acetate pharmacology, Myeloid Cells drug effects, Myeloid Cells physiology, Myometrium cytology, Obstetric Labor, Premature pathology, Pregnancy, Tissue Culture Techniques, Toll-Like Receptor 2 immunology, Toll-Like Receptor 2 metabolism, Toll-Like Receptor 4 immunology, Toll-Like Receptor 4 metabolism, Young Adult, Myometrium physiology, Obstetric Labor, Premature immunology, Obstetric Labor, Premature physiopathology, Toll-Like Receptor 2 genetics, Toll-Like Receptor 4 genetics
- Abstract
Objective: To define the role of Toll-like receptors (TLR-2, TLR-4) and triggering receptor expressed on myeloid cells-1 (TREM-1) in pregnant human myometrium., Study Design: Expression of TLR-2 and -4 mRNA and protein and TREM-1 mRNA was quantified in human myometrial samples. Subsequently, we investigated the effect of medroxyprogesterone acetate (MPA) as a functional inhibitor of the TLR agonist lipopolysaccharide (LPS)., Results: Messenger RNA expression of TLR-2 and -4 was significantly higher in myometrium at term compared with preterm (P = .009 and .016, respectively). Toll-like receptor-2 protein expression was significantly higher during labor (P = .002) compared with nonlaboring samples. Triggering receptor expressed on myeloid cells-1 mRNA expression was increased in both myometrium and cervix after labor at term (P < .05). Medroxyprogesterone acetate significantly suppressed LPS-induced production of interleukin 1b (IL-1b), IL-6, and IL-8 in vitro (P < .05)., Conclusion: Toll-like receptors 2and 4 and TREM-1 are expressed in human myometrium and may play a role in the mechanism of labor at term, and their functional effects are inhibited by MPA.
- Published
- 2009
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