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The attachment of Agrobacterium cells to wheat roots is an active process and it was possible to inhibit A. radiobacter attachment to wheat roots by different substances. The chvB Tn5 A. tumefaciens and Calcofluor-dark Tn5 A. radiobacter mutants poorly attached to wheat roots. The ability of A. rhizogenes LMG 150 to attach to the roots of various rice cultivars was different. The competitiveness deficient mutant of R. meliloti strain SHM-105 had the ability to attach to rice roots 6 times less as compared with the wild strain. Presumably, agrobacterial rhicadhesin do not participate directly in the attachment process to the monocotyledonous wheat root surface. The flagella of A. radiobacter are involved in attachment to the monocotyledonous cell surface, since non-motile, Fla minus T5 mutants of A. radiobacter 5D-1, have low attachment ability to wheat roots.