1. Detection of Pestivirus A (bovine viral diarrhea virus 1) in free-living wild boars in Brazil
- Author
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Tatiana C. G. D. de Souza, Raquel Arruda Leme, Amauri Alcindo Alfieri, Alice Fernandes Alfieri, Alais Maria Dall Agnol, and Gisele da Silva Porto
- Subjects
medicine.medical_specialty ,Genotype ,Swine ,animal diseases ,viruses ,Sus scrofa ,Animals, Wild ,Beef cattle ,Antibodies, Viral ,Microbiology ,complex mixtures ,Virus ,03 medical and health sciences ,Medical microbiology ,Wild boar ,biology.animal ,Media Technology ,medicine ,Animals ,Pathogen ,Lung ,Phylogeny ,030304 developmental biology ,Swine Diseases ,0303 health sciences ,biology ,030306 microbiology ,Diarrhea Virus 1, Bovine Viral ,Pestivirus ,Pestivirus Infections ,virus diseases ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Virology ,Veterinary Microbiology - Short Communication ,Herd ,biology.protein ,RNA, Viral ,Antibody ,5' Untranslated Regions ,Brazil - Abstract
Bovine viral diarrhea virus (BVDV) is a major pathogen in cattle herds. Considering the epidemiological importance of pestiviruses and the process of wild boar invasion in Brazil, this study aimed to investigate the presence of BVDV in free-living boars. Forty-nine free-living wild boars were collected by exotic wildlife controller agents in 2017 and 2018. The presence of BVDV antibodies was evaluated in 42 serum samples using the virus neutralization test, and the detection of BVDV RNA was performed from the 5′UTR genomic region by RT-PCR assay in 49 lung tissue samples followed by sequencing of amplicons. BVDV neutralizing antibodies in serum were not identified in any of the evaluated samples. However, 3/49 (6.12%) lung samples were positive for BVDV RNA and classified one as BVDV-1a and two as 1d subgenotype. This report identified BVDV RNA in free-living wild boars and these results should be considered in BVDV control programs, especially in extensive beef cattle rearing systems.
- Published
- 2021