1. Development of effective biotransformation process for benzohydroxamic acid production using Bacillus smithii IIIMB2907.
- Author
-
Sharma, Hitesh, Singh, Rahul Vikram, Ganjoo, Ananta, Kumar, Amit, Singh, Ravail, and Babu, Vikash
- Subjects
- *
BIOCONVERSION , *THERMOPHILIC bacteria , *HOT springs , *BACILLUS (Bacteria) , *AMIDASES , *SOIL sampling , *ACYLTRANSFERASES - Abstract
The present study entails the usefulness of thermophilic amidase-producing bacterium in the biotransformation of benzamide to benzohydroxamic acid (BHA). A bacterium Bacillus smithii IIIMB2907 was isolated from a soil sample collected from hot springs of Manikaran, Himachal Pradesh, India. The whole cells of the bacterium displayed versatile substrate specificity by exhibiting significant activity with a diverse range of amides. In addition, amidase from thermophilic bacterium was induced by adding Ɛ-caprolactam in the mineral base media. The optimum temperature and pH of acyltransferase activity of amidase enzyme were found to be 50 °C and 7.0, respectively. Interestingly, half-life (t1/2) of this enzyme was 17.37 h at 50 °C. Bench-scale production and purification of BHA was carried out at optimized conditions which resulted in the recovery of 64% BHA with a purity of 96%. Owing to this, the reported process in the present study can be considered of immense industrial significance for the production of BHA. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF