1. Sulfur alleviates arsenic toxicity by reducing its accumulation and modulating proteome, amino acids and thiol metabolism in rice leaves.
- Author
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Dixit, Garima, Singh, Amit Pal, Kumar, Amit, Dwivedi, Sanjay, Deeba, Farah, Kumar, Smita, Suman, Shankar, Adhikari, Bijan, Shukla, Yogeshwar, Trivedi, Prabodh Kumar, Pandey, Vivek, and Tripathi, Rudra Deo
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SULFUR , *ARSENIC , *PROTEOMICS , *AMINO acids , *SULFHYDRYL group - Abstract
Arsenic (As) contamination of water is a global concern and rice consumption is the biggest dietary exposure to human posing carcinogenic risks, predominantly in Asia. Sulfur (S) is involved in di-sulfide linkage in many proteins and plays crucial role in As detoxification. Present study explores role of variable S supply on rice leaf proteome, its inclination towards amino acids (AA) profile and non protein thiols under arsenite exposure. Analysis of 282 detected proteins on 2-DE gel revealed 113 differentially expressed proteins, out of which 80 were identified by MALDI-TOF-TOF. The identified proteins were mostly involved in glycolysis, TCA cycle, AA biosynthesis, photosynthesis, protein metabolism, stress and energy metabolism. Among these, glycolytic enzymes play a major role in AA biosynthesis that leads to change in AAs profiling. Proteins of glycolytic pathway, photosynthesis and energy metabolism were also validated by western blot analysis. Conclusively S supplementation reduced the As accumulation in shoot positively skewed thiol metabolism and glycolysis towards AA accumulation under AsIII stress. [ABSTRACT FROM AUTHOR]
- Published
- 2015
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