1. FLOURY ENDOSPERM15 encodes a glyoxalase I involved in compound granule formation and starch synthesis in rice endosperm.
- Author
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You, Xiaoman, Zhang, Wenwei, Hu, Jinlong, Jing, Ruonan, Cai, Yue, Feng, Zhiming, Kong, Fei, Zhang, Jie, Yan, Haigang, Chen, Weiwei, Chen, Xingang, Ma, Jing, Tang, Xiaojie, Wang, Peng, Zhu, Shanshan, Liu, Linglong, Jiang, Ling, and Wan, Jianmin
- Subjects
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GLYOXALASE , *RICE , *ENDOSPERM , *AMYLOPLASTS , *STARCH synthesis , *PYRUVALDEHYDE - Abstract
Key message: FLO15encodes a plastidic glyoxalase I protein, OsGLYI7, which affects compound starch granule formation and starch synthesis in rice endosperm.Abstract: Starch synthesis in rice (Oryza sativa) endosperm is a sophisticated process, and its underlying molecular machinery still remains to be elucidated. Here, we identified and characterized two allelic rice floury endosperm 15 (flo15) mutants, both with a white-core endosperm. The flo15 grains were characterized by defects in compound starch granule development, along with decreased starch content. Map-based cloning of the flo15 mutants identified mutations in OsGLYI7, which encodes a glyoxalase I (GLYI) involved in methylglyoxal (MG) detoxification. The mutations of FLO15/OsGLYI7 resulted in increased MG content in flo15 developing endosperms. FLO15/OsGLYI7 localizes to the plastids, and the in vitro GLYI activity derived from flo15 was significantly decreased relative to the wild type. Moreover, the expression of starch synthesis-related genes was obviously altered in the flo15 mutants. These findings suggest that FLO15 plays an important role in compound starch granule formation and starch synthesis in rice endosperm. [ABSTRACT FROM AUTHOR]
- Published
- 2019
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