1. Genome-wide association analysis of lead accumulation in maize.
- Author
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Zhao, Xiongwei, Liu, Yajuan, Wu, Wenmei, Li, Yuhua, Luo, Longxin, Lan, Yuzhou, Cao, Yanhua, Zhang, Zhiming, Gao, Shibin, Yuan, Guangsheng, Liu, Li, Shen, Yaou, Pan, Guangtang, and Lin, Haijian
- Subjects
LEAD ,CORN ,SINGLE nucleotide polymorphisms ,REGRESSION analysis ,GENE expression ,LEAD poisoning - Abstract
Large phenotypic variations in the lead (Pb) concentration were observed in grains and leaves of maize plants. A further understanding of inheritance of Pb accumulation may facilitate improvement of low-Pb-accumulating cultivars in maize. A genome-wide association study was conducted in a population of 269 maize accessions with 43,737 single-nucleotide polymorphisms (SNPs). The Pb concentrations in leaves and kernels of 269 accessions were collected in pot-culture and field experiments in years of 2015 and 2016. Significant differences in Pb accumulation were found among individuals under different environments. Using the structure and kinship model, a total of 21 SNPs significantly associated with the Pb accumulation were identified with
P < 2.28 × 10−5 and FDR < 0.05 in the pot-culture and field experiments across 2 years. Three SNPs on chromosome 4 had significant associations simultaneously with the Pb concentrations of kernels and leaves and were co-localized with the previously detected quantitative trait loci. Through ridge regression best linear unbiased prediction Pb accumulation in the association population, the prediction accuracies by cross validation were 0.18-0.59 and 0.17-0.64, depending on thek -fold and the size of the training population. The results are helpful for genetic improvement and genomic prediction of Pb accumulation in maize. [ABSTRACT FROM AUTHOR]- Published
- 2018
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