1. Identification of <italic>EPSTI1</italic> as a new potential biomarker for SLE based on GEO database.
- Author
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Yang, Yiying, Zhang, Huali, Xiao, Xiaoyu, and Guo, Muyao
- Abstract
Objective: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with highly heterogeneous. The aim of this study is to find the key genes in peripheral blood mononuclear cells (PBMCs) of SLE patients and to provide a new direction for the diagnosis and treatment of lupus.GSE121239, GSE50772, GSE81622, and GSE144390 mRNA expression profiles were obtained from the website of Gene Expression Omnibus (GEO), and differential expressed genes (DEGs) analysis was performed by
R . Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to elucidate signaling pathways for the DEGs. Real-time qPCR (RT-qPCR) was used to verify the key geneEPSTI1 in PBMCs of SLE patients. Finally, the correlation analysis and ROC curve analysis ofEPSTI1 for SLE were performed.A total of 12 upregulated DEGs were identified, includingMMP8, MX1, IFI44, EPSTI1, OAS1, OAS3, HERC5, IFIT1, RSAD2, USP18, IFI44L, andIFI27 . GO and KEGG pathway enrichment analysis showed that those DEGs were mainly concentrated in the response to virus and IFN signaling pathways. Real-time qPCR (RT-qPCR) revealed thatEPSTI1 was increased in PBMCs of SLE.EPSTI1 was positively correlated with SLEDAI score in SLE patients. Besides,EPSTI1 was positively correlated with T cell activation- or differentiation-associated genes (BCL6 andRORC ). Furthermore, ROC analyses provedEPSTI1 may have diagnostic value for SLE.Together,EPSTI1 was found to be a potential biomarker for SLE, closely related to T cell immune imbalance.Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Methods: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with highly heterogeneous. The aim of this study is to find the key genes in peripheral blood mononuclear cells (PBMCs) of SLE patients and to provide a new direction for the diagnosis and treatment of lupus.GSE121239, GSE50772, GSE81622, and GSE144390 mRNA expression profiles were obtained from the website of Gene Expression Omnibus (GEO), and differential expressed genes (DEGs) analysis was performed byR . Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to elucidate signaling pathways for the DEGs. Real-time qPCR (RT-qPCR) was used to verify the key geneEPSTI1 in PBMCs of SLE patients. Finally, the correlation analysis and ROC curve analysis ofEPSTI1 for SLE were performed.A total of 12 upregulated DEGs were identified, includingMMP8, MX1, IFI44, EPSTI1, OAS1, OAS3, HERC5, IFIT1, RSAD2, USP18, IFI44L, andIFI27 . GO and KEGG pathway enrichment analysis showed that those DEGs were mainly concentrated in the response to virus and IFN signaling pathways. Real-time qPCR (RT-qPCR) revealed thatEPSTI1 was increased in PBMCs of SLE.EPSTI1 was positively correlated with SLEDAI score in SLE patients. Besides,EPSTI1 was positively correlated with T cell activation- or differentiation-associated genes (BCL6 andRORC ). Furthermore, ROC analyses provedEPSTI1 may have diagnostic value for SLE.Together,EPSTI1 was found to be a potential biomarker for SLE, closely related to T cell immune imbalance.Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Results: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with highly heterogeneous. The aim of this study is to find the key genes in peripheral blood mononuclear cells (PBMCs) of SLE patients and to provide a new direction for the diagnosis and treatment of lupus.GSE121239, GSE50772, GSE81622, and GSE144390 mRNA expression profiles were obtained from the website of Gene Expression Omnibus (GEO), and differential expressed genes (DEGs) analysis was performed byR . Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to elucidate signaling pathways for the DEGs. Real-time qPCR (RT-qPCR) was used to verify the key geneEPSTI1 in PBMCs of SLE patients. Finally, the correlation analysis and ROC curve analysis ofEPSTI1 for SLE were performed.A total of 12 upregulated DEGs were identified, includingMMP8, MX1, IFI44, EPSTI1, OAS1, OAS3, HERC5, IFIT1, RSAD2, USP18, IFI44L, andIFI27 . GO and KEGG pathway enrichment analysis showed that those DEGs were mainly concentrated in the response to virus and IFN signaling pathways. Real-time qPCR (RT-qPCR) revealed thatEPSTI1 was increased in PBMCs of SLE.EPSTI1 was positively correlated with SLEDAI score in SLE patients. Besides,EPSTI1 was positively correlated with T cell activation- or differentiation-associated genes (BCL6 andRORC ). Furthermore, ROC analyses provedEPSTI1 may have diagnostic value for SLE.Together,EPSTI1 was found to be a potential biomarker for SLE, closely related to T cell immune imbalance.Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Conclusion: Systemic lupus erythematosus (SLE) is a chronic autoimmune disease with highly heterogeneous. The aim of this study is to find the key genes in peripheral blood mononuclear cells (PBMCs) of SLE patients and to provide a new direction for the diagnosis and treatment of lupus.GSE121239, GSE50772, GSE81622, and GSE144390 mRNA expression profiles were obtained from the website of Gene Expression Omnibus (GEO), and differential expressed genes (DEGs) analysis was performed byR . Then, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed to elucidate signaling pathways for the DEGs. Real-time qPCR (RT-qPCR) was used to verify the key geneEPSTI1 in PBMCs of SLE patients. Finally, the correlation analysis and ROC curve analysis ofEPSTI1 for SLE were performed.A total of 12 upregulated DEGs were identified, includingMMP8, MX1, IFI44, EPSTI1, OAS1, OAS3, HERC5, IFIT1, RSAD2, USP18, IFI44L, andIFI27 . GO and KEGG pathway enrichment analysis showed that those DEGs were mainly concentrated in the response to virus and IFN signaling pathways. Real-time qPCR (RT-qPCR) revealed thatEPSTI1 was increased in PBMCs of SLE.EPSTI1 was positively correlated with SLEDAI score in SLE patients. Besides,EPSTI1 was positively correlated with T cell activation- or differentiation-associated genes (BCL6 andRORC ). Furthermore, ROC analyses provedEPSTI1 may have diagnostic value for SLE.Together,EPSTI1 was found to be a potential biomarker for SLE, closely related to T cell immune imbalance.Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE .Key Points •EPSTI1 expression was significantly increased in PBMCs of SLE patients. • EPSTI1 was positively correlated with disease activity and T cell activation- or differentiation-associated genes in SLE patients .• EPSTI1 might have a good diagnostic value for SLE . [ABSTRACT FROM AUTHOR]- Published
- 2024
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