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The identification and accurate quantification of sources or sinks of greenhouse gas (GHG) have become a key challenge for scientists and policymakers working on climate change. The creation of a hydropower reservoir, while damming a river for power generation, converts the terrestrial ecosystems into aquatic and subsequently aerobic and anaerobic decomposition of flooded terrestrial soil organic matter resulting in the emission of significant quantity of GHG to the atmosphere. Tropical/subtropical hydropower reservoirs are more significant sources of GHG compared to boreal or temperate one. This paper aims to estimate the emission factor (gCO2eq./kWh) and net GHG emission from Koteshwar hydropower reservoir in Uttarakhand, India. Further, estimated GHG are compared with those from global reservoirs located in the same eco-region so that its impact could be timely minimized/mitigated. Results have shown that emission factor and net GHG emission of Koteshwar reservoir are, respectively, estimated as 13.87 gCO2eq./kWh and 167.70 Gg C year−1 which are less than other global reservoirs located in the same eco-region. This information could be helpful for the hydropower industries to construct reservoirs in tropical eco-regions. [ABSTRACT FROM AUTHOR]